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Citations for Akoya Biosciences :
101 - 150 of 162 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Fluorescent labeling was conducted using Opal 480 (GFAP) and Opal 570 (CD44) dyes (1:150; Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide Signal Amplification (TSA) solutions were prepared as follows: 1/500 for TSA-Fluorescein (Akoya Biosciences, NEL741001KT), 1/2000 for TSA-CY3 (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... All probes were detected using a 1:750 dilution of TSA plus fluorescein 488 (Akoya Biosciences NEL741001KT). Slides were then dried overnight ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained with probes against CCL2 (channel 1; ACD 423811) conjugated to Opal 690 (Akoya FP1497001KT), CDKN1A (channel 2 ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Neuroscience 2024Quote: ... FITC detection was performed using TSA Plus fluorescein diluted 1:50 in amplification diluent (NEL741001KT, Akoya Biosciences). Samples were washed several times in TNT ...
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bioRxiv - Cancer Biology 2023Quote: ... The slide was then transferred to the flow cell carrier and imaged on a PhenoCycler-Fusion (Akoya Biosciences, MA) using the following exposure settings ...
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bioRxiv - Cancer Biology 2023Quote: A T cell panel comprising five markers for five channels was generated for imaging with a Vectra (Akoya Biosciences) as previously described41 for validation purposes ...
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bioRxiv - Developmental Biology 2021Quote: ... Slices were covered by 80 μL of Opal 650 at 1/1000 (Opal 650 reagent pack, AKOYA Biosciences) during 30 min at 40°C and washed at RT with RNAscope wash buffer two times ...
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bioRxiv - Neuroscience 2022Quote: ... Hybridized slides were incubated with amplification reagents and Opal 570 and 690 dyes (Akoya Biosciences, 1:1500 dilution) followed by sequential incubation with tyrosine hydroxylase (Aves Labs ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated for 30 minutes with 1:200 Cy3-Tyramide in TSA amplification buffer (Akoya Biosciences®), at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the FITC-labeled probe was visualized using tyramide-FITC Plus (1/50 dilution, 15-minute incubation, Akoya Biosciences). Following washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL, Akoya Bioscience). Microscope images were taken using the Zeiss AxioImager M2 and analyzed using ZEN blue software (v2.3).
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Neuroscience 2023Quote: ... CasL/NEDD9 (ACD, 446311), and Sin/EFS (ACD, 806951) probes generated by Advanced Cell Diagnostics were uniquely amplified with Opal dyes (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Spectral unmixing and further tissue /cell segmentation as well as phenotyping were performed using the inForm Advanced Image Analysis software (inForm v2.4.10, Akoya Biosciences®). Multiplex data analyses ...
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bioRxiv - Neuroscience 2021Quote: In situ hybridization was performed with the RNAscope® Multiplex Fluorescent Assay v2 from Advanced Cell Diagnostics (ACD) combined with Tyramide Signal Amplification technology (TSA™) and dyes from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... based on canonical marker expression values and were confirmed via visual overlay of the cell populations on the immunofluorescent images utilizing the multiplex analysis viewer (MAV, Akoya Biosciences). Voronoi diagrams were created based on the spatial coordinates of the cell types and utilized to mathematically compute adjacent “cell-cell” contacts ...
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bioRxiv - Immunology 2023Quote: ... Slides were acquired on an automated slide scanning brightfield microscope (Vectra) and positive cells were quantified using inForm automated image analysis software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... A training set of 9 representative images was used to train analyses algorithms for tissue and cell segmentation and phenotyping using inForm software (Akoya Biosciences). Representative autofluorescence was measured on an unstained control slide and subtracted from study slides ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorophores applied to detect the signal from the probes were Opal 690 Reagent (1:750; Akoya Biosciences, #FP1497001KT) for C1 and Opal 570 Reagent (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Cell Biology 2021Quote: ... For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya). For unamplified targets appropriate Cy3 or FITC labeled secondary antibodies were used at 1:100 ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated in Opal 520 diluted in blocking buffer (for CK19 and CD8 primary) (Akoya Biosciences, 1:100) for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... ISH was completed by washing slides 3x for 15 minutes in PBS before incubation in Polaris 780 (1:125 in diluent buffer, Akoya) for 30 minutes at 40C ...
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bioRxiv - Neuroscience 2023Quote: ... Final detection was achieved with the Opal-570 and Opal-690 fluorophore reagent packs (Akoya BioSciences, Inc.,diluted 1:1000), and samples were conterstained with DAPI (ACD ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (1:1000) were assigned for each channel: Vgat expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), Npy expression was identified by Opal 570 (Akoya Bioscience ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... a working Opal dye solution was made fresh using a 1:500 ratio of Opal dye (Akoya Biosciences, Marlborough, MA, USA) to TSA buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... then covered by 80 μL of Opal 520 at 1/1500 (Opal 520 reagent pack, FP1487001KT, AKOYA Biosciences, Malborough, United States) during 30 min at 40°C and washed at RT with RNAscope wash buffer two times ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were further incubated with 1:200 TSA Plus FITC or Cy3 reagents for 3-5 minutes of signal development (NEL774001KT or NEL771B001KT, Akoya Bioscience). Sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunofluorescence was visualized using Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in a Plus Automation Amplification Diluent (Akoya biosciences). Serial multiplexing was performed by repeating the sequence of antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... 690 fluorophores according to the protocol using 1:750 dilution of each fluorophore (Cat #FP1488001, #FP1497001, #FP1501001, Akoya Biosciences, Marlborough, MA). Slides were then mounted using Prolong Gold anti-Fade mounting media with DAPI and imaged at 600X magnification using the Nikon Ti2-E inverted microscope system (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Neuroscience 2023Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was washed several more times in TNT and DIG detection was performed using TSA Plus Cyanine diluted 1:50 in amplification diluent (NEL744001KT, Akoya Biosciences). Fluorescently labeled samples were imaged using confocal microscopy.
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bioRxiv - Genomics 2024Quote: ... Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in 1x Plus Automation Amplification Diluent (Akoya biosciences) for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...