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Citations for Akoya Biosciences :
1 - 50 of 260 citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... For multiplexed IHC staining the Opal 6-plex Detection Kit (Akoya Biosciences) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplexed staining was performed using the Opal 6-plex detection kit (Akoya Biosciences NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences, Marlborough, MA, USA) were employed for the staining process ...
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bioRxiv - Cancer Biology 2023Quote: ... which was performed using an Opal 6-Plex Detection Kit (Akoya Biosciences, Menlo Park, CA) with the following Abs ...
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bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Multispectral fluorescence staining was performed using the Opal 6-Plex Detection kit (Akoya Biosciences, Marlborough, MA) using formalin-fixed ...
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bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Cancer Biology 2024Quote: ... the 6-plex TSA with Opal dyes (Akoya) 6-plex and the 8-plex Signal Star (CST) ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 650 (1:500, Akoya FP1496001KT) reagent incubation for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Immunology 2023Quote: ... followed by probing with an appropriate OPAL fluorophore-conjugated secondary Ab (Akoya Biosciences, Supplementary Table 6). This was followed by a further HIER to remove the primary Ab before staining with the next primary/secondary Ab combination ...
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bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
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bioRxiv - Developmental Biology 2023Quote: ... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...
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bioRxiv - Cancer Biology 2023Quote: ... 2x for 2 min and incubated in CODEX staining buffer (Akoya Biosciences, 232106) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... CDKN1A (channel 2; ACD 311401-C2) conjugated to Opal Polaris 780 (Akoya FP1501001KT), and IL1B (channel 4 ...
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bioRxiv - Neuroscience 2023Quote: ... assay # 2: VIP expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), CCK expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with spectral DAPI (4,6-diamidino-2-phenylindole) solution (Akoya Biosciences). Finally ...
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bioRxiv - Pathology 2022Quote: ... using CODEX conjugation kit (Cat#: 7000009, Akoya) following CODEX conjugation manual.
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bioRxiv - Neuroscience 2021Quote: ... 570 and 690 reagent kits (Akoya Biosciences). Cells were stained with DAPI (ACD ...
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bioRxiv - Developmental Biology 2023Quote: ... using TSA Plus amplification kits (Akoya Biosciences) and mouse anti-β-galactosidase (Promega #Z378 ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... TSA Plus Cyanine 5 Kit (Akoya Biosciences, NEL745001KT) was used ...
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bioRxiv - Developmental Biology 2021Quote: ... using the TSA Plus fluorescein detection kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... or TSA Plus Fluorescein kit (Akoya Biosciences NEL741001KT) were used to amplify signal for immunohistochemistry or immunofluorescence ...
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bioRxiv - Cancer Biology 2022Quote: ... using OPAL detection kits (ref NEL871001KT, AKOYA bioscience). Primary antibodies specific to Survivin (1/400 ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...