Labshake search
Citations for Akoya Biosciences :
1 - 50 of 207 citations for Biotin Z Antibody Internalization Kit rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... and Opal 650 and TSA-biotin (TSA Plus Biotin Kit) (Akoya Biosciences) and streptavidin-conjugated Atto 425 (Sigma ...
-
bioRxiv - Developmental Biology 2023Quote: ... A Tyramide Biotin Signal Amplification (TSA) kit (Akoya/Perkin Elmer) was used for PRDM16 signal amplification in combination with Streptavidin ...
-
bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
-
bioRxiv - Immunology 2021Quote: TSA Biotin (Akoya, NEL749A001KT) deposition ...
-
bioRxiv - Systems Biology 2023Quote: ... Proximal protein biotinylation occurs with treatment of H2O2 and tyramide-biotin (TSA Biotin System, Akoya Bioscience NEL700A001KT), resulting in tyramide-biotin radicalization and covalent deposition onto proximal proteins ...
-
bioRxiv - Neuroscience 2022Quote: ... amplification was achieved using TSA Biotin (Akoya Bioscience). For fluorescence double in situ hybridization ...
-
bioRxiv - Neuroscience 2023Quote: ... Signals were amplified by TSA-plus Biotin (AKOYA Bioscience ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
-
bioRxiv - Immunology 2021Quote: ... Reagents for TSA hapten deposition were Biotin (Akoya, NEL749A001KT) and DIG (Akoya ...
-
bioRxiv - Immunology 2023Quote: ... and C3 oligos with TSA-Biotin (Akoya Biosciences, NEL749A001KT) diluted 1:100 in TSA buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated in TSA-plus biotin (NEL749A001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
-
bioRxiv - Physiology 2023Quote: Immunohistochemical staining for insulin and glucagon was conducted using a Tyramide Signal Amplification (TSA) Plus Biotin kit according to the manufacturer’s protocol (NEL700A001KT, Akoya Biosciences, Marlborough, MA, USA), as previously described [15] ...
-
bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with anti-rabbit HRP-conjugated secondary antibody (Akoya Biosciences, NEL840001KT). Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2021Quote: ... we performed TSA reaction (TSA plus biotin, Akoya Biosciences, Marlborough, MA) and ABC reaction (Vector laboratories ...
-
bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
-
bioRxiv - Bioengineering 2022Quote: ... All sections were then incubated with an Opal 520 polymer anti-rabbit HRP antibody (Akoya Biosciences) and counterstained with DAPI ...
-
bioRxiv - Neuroscience 2020Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
-
bioRxiv - Developmental Biology 2022Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... was used to detect the Flu-labeled RNA probe by TSA-plus biotin (AKOYA Bioscience ...
-
bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... required the tyramide signal amplification (TSA) biotin system following the manufacturer’s protocol (AKOYA Biosciences #NEL 700A001KT). Slides were counterstained with hematoxylin (Ventana 760-2021 ...
-
bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
-
bioRxiv - Neuroscience 2024Quote: ... was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidin-AF488 (at a dilution of 1:250 ...
-
bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
-
bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Low amounts of antigens (CD32, ACE) were revealed by Tyramide signal amplification (TSA) biotin or fluorescence amplification systems (Akoya, Biosciences). An isotype-matched negative control was performed for each immunostaining ...
-
bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
-
bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
-
bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
-
bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
-
bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
-
bioRxiv - Cancer Biology 2023Quote: ... NRG1 and TGFa primary antibodies were detected with HRP-conjugated antibody (ARR1001KT, Akoya Biosciences) and TSA-based amplification with Opal dye 690 (FP1497001KT ...
-
bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
-
bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
-
bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated anti-flu antibody (AKOYA Bioscience NEF710001EA ...
-
bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: Antibody conjugation requires reagents from Akoya Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
-
bioRxiv - Developmental Biology 2024Quote: The high-parametric antibody panel consisted of both a selection of pre-conjugated antibodies (n=23, Akoya Biosciences) and antibodies available through other commercial sources that were conjugated in house (n=49 ...
-
bioRxiv - Cell Biology 2022Quote: ... a secondary HRP antibody (ARH1001EA, AKOYA Biosciences) followed by Opal 520 fluorophore (FP1487001KT ...
-
bioRxiv - Neuroscience 2022Quote: ... diluted in Antibody Diluent/Block (Akoya, ARD1001EA) for 30min at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...