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Citations for Akoya Biosciences :
1 - 50 of 150 citations for 8H INDENO 1 2 D THIAZOL 2 AMINE HYDROBROMIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Developmental Biology 2023Quote: ... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 650 (1:500, Akoya FP1496001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...
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bioRxiv - Cancer Biology 2023Quote: ... 2x for 2 min and incubated in CODEX staining buffer (Akoya Biosciences, 232106) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... CDKN1A (channel 2; ACD 311401-C2) conjugated to Opal Polaris 780 (Akoya FP1501001KT), and IL1B (channel 4 ...
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bioRxiv - Neuroscience 2023Quote: ... assay # 2: VIP expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), CCK expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with spectral DAPI (4,6-diamidino-2-phenylindole) solution (Akoya Biosciences). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was then allowed to dry for 2 minutes and hydrated in hydration buffer (Akoya Biosciences) twice for 2 minutes ...
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bioRxiv - Immunology 2022Quote: ... several steps are carried out in order to amplify the IL-22BP signal: polymer enhancer (M023 from ImPath) then HRP-2 (M024 from ImPath) and finally Opal 650 (FP1496A from Akoya) following manufacturer’s instruction ...
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bioRxiv - Pathology 2023Quote: ... Spatial co-localization of CD34 and fibrinogen was carried out using the 2-color IHC images obtained with the Mantra 2.0 microscope (Akoya Biosciences) with spectral unmixing using Inform software ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked with 10% BSA + 0.05% Tween 20 and the antibodies listed in Supplementary Table 4 were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner using the OPAL reagents (Akoya Biosciences). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2021Quote: ... Opal™ 570 (Akoya; #FP1488001KT; 1:1500) was applied for channel 1 and Opal™ 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 690 dyes (1:1000, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... 1/2000 for TSA-CY3 (Akoya Biosciences, NEL744001KT) and 1/1000 for TSA-CY5 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and 690 fluorophores (Akoya Biosciences, diluted 1:200) respectively ...
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bioRxiv - Immunology 2022Quote: ... Opal 570 reagent 1:100 (NEL810001KT, Akoya Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Amplification and HRP-C1 and HRP-C2 signal development were performed as recommended by the manufacturer with the modification of the use of 0.5× RNAScope wash buffer instead of 1× RNAScope wash buffer and use of a 1:150 dilution of Opals (all from Akoya Bioscience) instead of 1:1500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Reporter stock solution was prepared containing 1:300 Hoechst stain and 1:12 dilution of assay reagent in 1x CODEX buffer (Akoya Bioscience). Fluorescent oligonucleotides (Akoya Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... The hybridization detection was carried out using Opal Fluorophore Reagent Packs Opal-520 (channel (C) 1)/570 (C2)/690 (C3) (1:1500) (Akoya Biosciences). Probes (Advanced Cell Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... channel 1 was established and the slices were incubated in a 1:750 solution of Opal 570 (Akoya Biosciences, catalog # FP1488001KT) diluted in TSA Buffer (ACD) ...