Labshake search
Citations for Akoya Biosciences :
1 - 50 of 79 citations for 7 1H Pteridinone 4 amino 2 6 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
-
bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
-
bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
-
bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the Opal 7-Color IHC kit (Akoya Biosciences). 4μm thick FFPE tissue sections were baked at 60°C for 1 hour prior to deparaffinization in xylene and rehydration in a series of graded ethanol ...
-
bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions (Table S9) ...
-
bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions using antibodies shown at Table S9 ...
-
bioRxiv - Cell Biology 2023Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies p-HDAC4Ser632 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies AQP1 ...
-
bioRxiv - Pathology 2022Quote: ... We adopted Opal PolarisTM 7-color Manual IHC Kit (NEL861001KT, Akoya). Primary antibodies including HERV (LS-C65284 ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... the 6-plex TSA with Opal dyes (Akoya) 6-plex and the 8-plex Signal Star (CST) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer ...
-
bioRxiv - Cancer Biology 2021Quote: Multiplex IHC was performed using an Opal 7-Color IHC Kit (Akoya Biosciences) on a Bond RX automated stainer (Leica Biosystems) ...
-
bioRxiv - Immunology 2022Quote: ... Staining was performed using the Opal™ 7-Color Automatic IHC Kit (Akoya) according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2021Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...
-
bioRxiv - Genomics 2024Quote: ... For multiplexed IHC staining the Opal 6-plex Detection Kit (Akoya Biosciences) was used ...
-
bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
-
bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
-
bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Multiplexed staining was performed using the Opal 6-plex detection kit (Akoya Biosciences NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
-
bioRxiv - Cell Biology 2023Quote: ... and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences, Marlborough, MA, USA) were employed for the staining process ...
-
bioRxiv - Cancer Biology 2023Quote: ... which was performed using an Opal 6-Plex Detection Kit (Akoya Biosciences, Menlo Park, CA) with the following Abs ...
-
bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
-
bioRxiv - Molecular Biology 2023Quote: Multispectral fluorescence staining was performed using the Opal 6-Plex Detection kit (Akoya Biosciences, Marlborough, MA) using formalin-fixed ...
-
bioRxiv - Immunology 2023Quote: ... followed by probing with an appropriate OPAL fluorophore-conjugated secondary Ab (Akoya Biosciences, Supplementary Table 6). This was followed by a further HIER to remove the primary Ab before staining with the next primary/secondary Ab combination ...
-
bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
-
bioRxiv - Cell Biology 2022Quote: ... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... the OPAL 4-Color Manual IHC Kit (Akoya Biosciences NEL810001KT) was used ...
-
bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
-
bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...
-
bioRxiv - Immunology 2021Quote: ... we stained slides using Opal 4-Color IHC Kits (AKOYA Biosciences) and observed using the Zeiss LSM 710 or LSM 880 confocal microscope and ZEN microscope software (Carl Zeiss) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The PerkinElmer Opal 4-Color Manual IHC Kit (PerkinElmer/Akoya, NEL810001KT) was used for the tyramide signal amplification according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were stained using Opal 4-Color IHC kit (Akoya, NEL810001KT) according to manufacturer’s instructions with the following primary antibodies ...
-
bioRxiv - Developmental Biology 2023Quote: ... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
-
bioRxiv - Cancer Biology 2020Quote: ... included in the Opal 4 color kit (Cat No. NEL810001KT, AKOYA Biosciences) to detect antibody staining ...
-
bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
-
bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
-
bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
-
bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
-
bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2x for 2 min and incubated in CODEX staining buffer (Akoya Biosciences, 232106) for 20 min ...