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Citations for Akoya Biosciences :
101 - 150 of 239 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... The probes were for Mus musculus Fgf10 (channel 2, 446371) and Wnt5a (channel 2, 316791) and the fluorophores were Opal Polaris 520 and Opal 620 (Akoya Biosciences FP1487001KT and FP1495001KT) ...
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bioRxiv - Neuroscience 2023Quote: ... signals were amplified with TSA-plus Cyanine 5 (NEL745001KT, Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound probes were detected using TSA Plus fluorophores Cyanine 5 (Akoya Bioscience, NEL745001KT) and Cyanine 3 (Akoya Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Pathology 2021Quote: IHC tissues were imaged with a Mantra 2 Quantitative Pathology Workstation (Akoya Biosciences) with a 20x objective ...
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bioRxiv - Cancer Biology 2023Quote: ... 2x for 2 min and incubated in CODEX staining buffer (Akoya Biosciences, 232106) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... CDKN1A (channel 2; ACD 311401-C2) conjugated to Opal Polaris 780 (Akoya FP1501001KT), and IL1B (channel 4 ...
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bioRxiv - Neuroscience 2023Quote: ... assay # 2: VIP expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), CCK expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with spectral DAPI (4,6-diamidino-2-phenylindole) solution (Akoya Biosciences). Finally ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed before ISH signal was developed with the TSA Plus Cyanine 5 System (Akoya Biosciences # NEL745001KT). Slides were then stripped of horseradish peroxidase and blocked with normal goat serum before incubating with goat anti-rabbit Alexa Fluor 488 (1:400 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence signals were amplified and visualized using the TSA Plus Cyanine-5 and the TSA Plus Fluorescein systems (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 5 ROIs in 34 WSI from the melanoma dataset were spectrally unmixed using inForm® software v6.4.2 (Akoya Biosciences), and analyzed with the HALO® image analysis platform (Indica Labs) ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was then allowed to dry for 2 minutes and hydrated in hydration buffer (Akoya Biosciences) twice for 2 minutes ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Genomics 2021Quote: ... 323100) with the V-nCoV2019-S-C3 probe (ACD Bio, 848561-C3) and the TSA Plus Cyanine 5 fluorophore (Akoya Biosciences, NEL745001KT). For samples D13-17 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer and incubated with Cy5-tyramide solution (TSA Plus Cyanine 5; AKOYA Biosciences, NEL745001KT) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Immunology 2022Quote: ... several steps are carried out in order to amplify the IL-22BP signal: polymer enhancer (M023 from ImPath) then HRP-2 (M024 from ImPath) and finally Opal 650 (FP1496A from Akoya) following manufacturer’s instruction ...
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bioRxiv - Pathology 2023Quote: ... Spatial co-localization of CD34 and fibrinogen was carried out using the 2-color IHC images obtained with the Mantra 2.0 microscope (Akoya Biosciences) with spectral unmixing using Inform software ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked with 10% BSA + 0.05% Tween 20 and the antibodies listed in Supplementary Table 4 were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner using the OPAL reagents (Akoya Biosciences). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2021Quote: ... Opal™ 570 (Akoya; #FP1488001KT; 1:1500) was applied for channel 1 and Opal™ 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 690 dyes (1:1000, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... 1/2000 for TSA-CY3 (Akoya Biosciences, NEL744001KT) and 1/1000 for TSA-CY5 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and 690 fluorophores (Akoya Biosciences, diluted 1:200) respectively ...
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bioRxiv - Immunology 2022Quote: ... Opal 570 reagent 1:100 (NEL810001KT, Akoya Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Amplification and HRP-C1 and HRP-C2 signal development were performed as recommended by the manufacturer with the modification of the use of 0.5× RNAScope wash buffer instead of 1× RNAScope wash buffer and use of a 1:150 dilution of Opals (all from Akoya Bioscience) instead of 1:1500 ...