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Citations for Akoya Biosciences :
101 - 150 of 240 citations for 6 Oxabicyclo 3.1.0 hexane 2 ethanol 4 4 difluoro 3 hydroxy 1S 1 α 2 bta 3 bta 5 α 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then rinsed through two rounds of incubation in distilled water for 2 minutes and stored in Hydration Buffer from the PhenoCycler Staining Kit (Akoya Biosciences, MA, #7000008) until ready for staining.
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL, Akoya Bioscience). Microscope images were taken using the Zeiss AxioImager M2 and analyzed using ZEN blue software (v2.3).
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bioRxiv - Genomics 2024Quote: ... For multiplexed IHC staining the Opal 6-plex Detection Kit (Akoya Biosciences) was used ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... Multiplexed staining was performed using the Opal 6-plex detection kit (Akoya Biosciences NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences, Marlborough, MA, USA) were employed for the staining process ...
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bioRxiv - Cancer Biology 2023Quote: ... which was performed using an Opal 6-Plex Detection Kit (Akoya Biosciences, Menlo Park, CA) with the following Abs ...
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bioRxiv - Cancer Biology 2024Quote: The multi-IF was performed using the Opal 6-Plex Manual Detection Kit (NEL811001KT, Akoya), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... TSA Plus Cyanine 5 Kit (Akoya Biosciences, NEL745001KT) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... a 5 min DAPI staining (Akoya Biosciences, #FP1490) was performed before mounting with ProLong Diamond Antifade media (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Multispectral fluorescence staining was performed using the Opal 6-Plex Detection kit (Akoya Biosciences, Marlborough, MA) using formalin-fixed ...
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bioRxiv - Immunology 2023Quote: ... followed by probing with an appropriate OPAL fluorophore-conjugated secondary Ab (Akoya Biosciences, Supplementary Table 6). This was followed by a further HIER to remove the primary Ab before staining with the next primary/secondary Ab combination ...
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bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
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bioRxiv - Microbiology 2022Quote: ... and TSA Plus-Cyanine 5 (AKOYA Biosciences, cat# NEL745001KT), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... signals were amplified with TSA-plus Cyanine 5 (NEL745001KT, Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound probes were detected using TSA Plus fluorophores Cyanine 5 (Akoya Bioscience, NEL745001KT) and Cyanine 3 (Akoya Bioscience ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed before ISH signal was developed with the TSA Plus Cyanine 5 System (Akoya Biosciences # NEL745001KT). Slides were then stripped of horseradish peroxidase and blocked with normal goat serum before incubating with goat anti-rabbit Alexa Fluor 488 (1:400 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence signals were amplified and visualized using the TSA Plus Cyanine-5 and the TSA Plus Fluorescein systems (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 5 ROIs in 34 WSI from the melanoma dataset were spectrally unmixed using inForm® software v6.4.2 (Akoya Biosciences), and analyzed with the HALO® image analysis platform (Indica Labs) ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Genomics 2021Quote: ... 323100) with the V-nCoV2019-S-C3 probe (ACD Bio, 848561-C3) and the TSA Plus Cyanine 5 fluorophore (Akoya Biosciences, NEL745001KT). For samples D13-17 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer and incubated with Cy5-tyramide solution (TSA Plus Cyanine 5; AKOYA Biosciences, NEL745001KT) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2021Quote: ... Opal™ 570 (Akoya; #FP1488001KT; 1:1500) was applied for channel 1 and Opal™ 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 690 dyes (1:1000, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Developmental Biology 2022Quote: ... 1/2000 for TSA-CY3 (Akoya Biosciences, NEL744001KT) and 1/1000 for TSA-CY5 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and 690 fluorophores (Akoya Biosciences, diluted 1:200) respectively ...
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bioRxiv - Immunology 2022Quote: ... Opal 570 reagent 1:100 (NEL810001KT, Akoya Biosciences) for 10 minutes ...