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Citations for Akoya Biosciences :
1 - 50 of 227 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Systems Biology 2023Quote: ... 6-diamidino-2-phenylindole (Spectral DAPI, Akoya Biosciences) was applied per provided protocols to label nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:750 dilution of TSA-Cyanine 3 Plus (AKOYA). The RNAscope® 3-plex LS Multiplex Negative Control Probe (Bacillus subtilis dihydrodipicolinate reductase (dapB ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Cell Biology 2022Quote: ... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... the OPAL 4-Color Manual IHC Kit (Akoya Biosciences NEL810001KT) was used ...
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bioRxiv - Microbiology 2022Quote: ... and 1:750 dilution of TSA Plus-Cyanine 3 (AKOYA Biosciences, cat# NEL744001KT) and TSA Plus-Cyanine 5 (AKOYA Biosciences ...
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bioRxiv - Immunology 2021Quote: ... we stained slides using Opal 4-Color IHC Kits (AKOYA Biosciences) and observed using the Zeiss LSM 710 or LSM 880 confocal microscope and ZEN microscope software (Carl Zeiss) ...
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bioRxiv - Cancer Biology 2021Quote: ... The PerkinElmer Opal 4-Color Manual IHC Kit (PerkinElmer/Akoya, NEL810001KT) was used for the tyramide signal amplification according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... sections were stained using Opal 4-Color IHC kit (Akoya, NEL810001KT) according to manufacturer’s instructions with the following primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with TSA-plus Cyanine 3 (NEL744001KT; Akoya Biosciences; 1:1500). Fluoromount (K024 ...
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bioRxiv - Cancer Biology 2020Quote: ... included in the Opal 4 color kit (Cat No. NEL810001KT, AKOYA Biosciences) to detect antibody staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 650 (1:500, Akoya FP1496001KT) reagent incubation for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Signal amplification was done with 1:50 TSA Plus Cyanine-3 or -5 (Akoya Biosciences) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were amplified by a 1:70 TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
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bioRxiv - Neuroscience 2022Quote: ... and 1hr incubation with TSA plus Cyanine-3 (1:300; cat# NEL744001KT; Akoya Biosciences, Marlborough, MA) was performed ...
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bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... washed again (2 x 5 min) and incubated with OPAL 650 Tyramide (Akoya Biosciences, FP1496001KT, 1:500) for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... and SARS-CoV-2 spike mRNA with TSA Plus CY5 (1:1500, #NEL745001KT, Akoya Biosciences, MA, USA). Fluorescent images were acquired with NanoString GeoMX DSP at 20×.
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bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... visualization of 4-color opal slides was performed by Vectra Polaris Quantitative Pathology Imaging Systems (Akoya, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were further incubated with 1:200 TSA Plus FITC or Cy3 reagents for 3-5 minutes of signal development (NEL774001KT or NEL771B001KT, Akoya Bioscience). Sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... of HNSCC tumors were sectioned to 4-μm thick for the subsequent multiplex immunohistochemistry via the OPAL Polaris system (Akoya Biosciences). After deparaffinization and hydration ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence staining of AKPT tumours was performed on 4 μm thick FFPE sections using the OPAL protocol (Akoya Biosciences, Marlborough, MA). The Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Multiplex immunofluorescence (MPIF) staining was performed on formalin-fixed paraffin embedded (FFPE) sections of thickness 4-µm using the OPAL protocol (Akoya Biosciences, Marlborough, MA) on the Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Cancer Biology 2024Quote: ... the 6-plex TSA with Opal dyes (Akoya) 6-plex and the 8-plex Signal Star (CST) ...
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...