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Citations for Akoya Biosciences :
51 - 90 of 90 citations for 6 Chloropyridine 3 4 diamine hydroChloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The signals were amplified by a 1:70 TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) for 25 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... sections following HPR-C1 treatment were incubated with TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Neuroscience 2022Quote: ... and 1hr incubation with TSA plus Cyanine-3 (1:300; cat# NEL744001KT; Akoya Biosciences, Marlborough, MA) was performed ...
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bioRxiv - Cancer Biology 2023Quote: Seven color multiplex-stained slides were imaged using the Vectra Multispectral Imaging System version 3 (Akoya). Scanning was performed at 20x (200x final magnification) ...
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bioRxiv - Molecular Biology 2023Quote: ... the development reaction was carried out using the TSA Plus Cyanine 3 Kit (Akoya Biosciences, US) (FISH ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed and analysed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... or the tyramide signal amplification (TSA) fluorophores (TSA Fluorescein, TSA Cyanine 3, TSA Cyanine 5) (Akoya Biosciences) were used for detection ...
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bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Developmental Biology 2024Quote: ... after which the second color was generated by Cyanine 3 incubation (NEL744001KT, Akoya biosciences, Marlborough, MA, USA) for five minutes ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were imaged at both 10x and 20x using the Vectra 3 Polaris and Vectra imaging software (Akoya Biosciences). Acquired qpTIFF images from the Vectra Polaris system were imported into QuPath analysis software(84 ...
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bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... visualization of 4-color opal slides was performed by Vectra Polaris Quantitative Pathology Imaging Systems (Akoya, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Immunology 2021Quote: ... Multispectral images were acquired using a Vectra® 3 imaging system employing the Vectra3 and Phenochart software (all Akoya Biosciences). Images are displayed in pseudocolours ...
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Developmental Biology 2023Quote: ... followed by streptavidin-conjugated peroxidase incubation and signal amplification using the TSA Plus Cyanine-3 or -5 kits (Akoya Biosciences).
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were further incubated with 1:200 TSA Plus FITC or Cy3 reagents for 3-5 minutes of signal development (NEL774001KT or NEL771B001KT, Akoya Bioscience). Sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then washed for 3 x 5 minutes in NTT and developed with Cy5-Tyramide signal amplification reagent (TSA Plus Kit, Akoya Biosciences #NEL745001KT) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 3 times in PBS and incubated in final fixative solution (1000uL of PBS + 20uL of Akoya’s final fixation reagent) for 20 minutes at room temperature.
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... of HNSCC tumors were sectioned to 4-μm thick for the subsequent multiplex immunohistochemistry via the OPAL Polaris system (Akoya Biosciences). After deparaffinization and hydration ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence staining of AKPT tumours was performed on 4 μm thick FFPE sections using the OPAL protocol (Akoya Biosciences, Marlborough, MA). The Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Cancer Biology 2022Quote: Multiplex immunofluorescence (MPIF) staining was performed on formalin-fixed paraffin embedded (FFPE) sections of thickness 4-µm using the OPAL protocol (Akoya Biosciences, Marlborough, MA) on the Leica BOND RXm autostainer (Leica Microsystems ...