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Citations for Akoya Biosciences :
1 - 50 of 250 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20 minute incubation with AR6 buffer (Akoya) at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 20 minute incubation with AR6 buffer (Akoya) at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... *20/0.8 objective (3DHISTECH) or with a Phenocycler scanner (Akoya Biosciences). Images were analyzed using QuPath software (Bankhead et al ...
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bioRxiv - Cell Biology 2021Quote: ... in light-protected 96-well plates sealed with foil (Akoya Biosciences) and automated image acquisition and fluidics exchange were performed using the Akoya CODEX instrument and CODEX Instrument Manager (CIM ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 15 minutes at 20% power in AR6 Buffer (Akoya Biosciences, AR600250ML) to affix Opal to target sites and remove primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Imaging was performed at 20× using a Vectra multispectral imaging system (Akoya Biosciences) and a spectral library was generated to separate spectral curves for each fluorophore ...
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bioRxiv - Cancer Biology 2023Quote: Multispectral imaging was performed using PhenoImager at 20× magnification (Akoya Biosciences, Marlborough, MA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: Stained coverslips were mounted onto the CODEX stage plate version 2 (Akoya) and secured onto the stage of a BZ-X810 inverted fluorescence microscope (Keyence) ...
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were scanned at ×20 on a Vectra3 multispectral imaging system (Akoya Biosciences, Marlborough, MA, USA). Epithelial areas were identified and segmented by machine learning using the panCK marker and HALO software (Indica Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... the final fixative was prepared by mixing 20 µL of CODEX final fixative (Akoya Biosciences, 232112) in 1000 µ L of 1x PBS ...
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bioRxiv - Immunology 2022Quote: ... Multispectral images were acquired at ×20 magnification using the Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya). MoTIF settings were used for multispectral image acquisition ...
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bioRxiv - Immunology 2022Quote: ... Multispectral images were acquired at ×20 magnification using the Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya) or COMET (Lunaphore) ...
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Opal Multiplex IF Kit (Akoya) was used to perform multiplex immunofluorescence according to manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2023Quote: ... H&E slides were viewed by a gastrointestinal pathologist to determine representative areas for multispectral image capture at 20 magnification using VECTRA® (PerkinElmer, now Akoya Biosciences). As capturing lymph node or lymphoid aggregates would potentially skew results ...
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bioRxiv - Pathology 2022Quote: ... using CODEX conjugation kit (Cat#: 7000009, Akoya) following CODEX conjugation manual.
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bioRxiv - Neuroscience 2021Quote: ... 570 and 690 reagent kits (Akoya Biosciences). Cells were stained with DAPI (ACD ...
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bioRxiv - Developmental Biology 2023Quote: ... using TSA Plus amplification kits (Akoya Biosciences) and mouse anti-β-galactosidase (Promega #Z378 ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Neuroscience 2022Quote: ... CODEX reporter 96-well plates were prepared using the cycle reporter configuration as outlined in the CODEX user manual (Akoya Biosciences, 2021a). Imaging was performed using a Keyence BZ-X800 epifluorescence microscope equipped with either a 20x objective (Nikon CFI Plan Apo 20x/0.75 ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... TSA Plus Cyanine 5 Kit (Akoya Biosciences, NEL745001KT) was used ...
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bioRxiv - Developmental Biology 2021Quote: ... using the TSA Plus fluorescein detection kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... or TSA Plus Fluorescein kit (Akoya Biosciences NEL741001KT) were used to amplify signal for immunohistochemistry or immunofluorescence ...
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bioRxiv - Cancer Biology 2022Quote: ... using OPAL detection kits (ref NEL871001KT, AKOYA bioscience). Primary antibodies specific to Survivin (1/400 ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Developmental Biology 2022Quote: ... or a Tyramide Signal Amplification kit (Akoya Biosciences, #NEL742001KT) following manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2022Quote: ... Immunohistochemistry was performed using Opal IHC kits (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and stained with the CODEX Staining Kit (Akoya Biosciences) in uncoated 6-well tissue culture plates (VWR ...
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bioRxiv - Cancer Biology 2022Quote: ... with the Opal 7-Color IHC kit (Akoya Biosciences). 4μm thick FFPE tissue sections were baked at 60°C for 1 hour prior to deparaffinization in xylene and rehydration in a series of graded ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... an Opal 3-Plex Manual Detection Kit (Akoya Biosciences) was used following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions (Table S9) ...
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bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions using antibodies shown at Table S9 ...
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bioRxiv - Cell Biology 2022Quote: ... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... The slides were finally incubated with TSA_Plus_Fluorescence Kits (Akoya, NEL745001KT) according to the manufacturer’s instruction and imaged.
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bioRxiv - Developmental Biology 2023Quote: ... A Tyramide Biotin Signal Amplification (TSA) kit (Akoya/Perkin Elmer) was used for PRDM16 signal amplification in combination with Streptavidin ...