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Citations for Akoya Biosciences :
151 - 200 of 268 citations for Human Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... detected by the fluorophore opal 520 at 1:200 (Akoya, ref. FP1487001KT) for 8 minutes ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were blocked with the blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... CA) using probes against the leptin receptor mRNA (Cat# 415951) and Opal 650 dye kit (Akoya Biosciences, Marlborough, MA) to label LepR mRNA ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... mIF was performed according to the manufacturer’s protocol using the OPAL Multiplex Manual IHC kit and antigen retrieval (AR) buffers AR6 and AR9 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... TSA reagents were diluted with 1×□Plus Amplification Diluent (PerkinElmer/Akoya Biosciences, US).
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 690 fluorophore (Akoya Biosciences, 1:50). Finally ...
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 570 fluorophore (Akoya Biosciences, 1:1000), and lastly CD3 (abcam ...
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bioRxiv - Microbiology 2022Quote: ... and 1:750 dilution of TSA Plus-Cyanine 3 (AKOYA Biosciences, cat# NEL744001KT) and TSA Plus-Cyanine 5 (AKOYA Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Slides were then incubated with TSA Plus (Opal 570, 1:100, Akoya Biosciences) for 10 minutes at room temperature ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... diluted 1:50 in amplification buffer (1X Plus Amplification Diluent, Akoya Biosciences, FP1135). Next ...
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bioRxiv - Neuroscience 2023Quote: ... The vGLUT2 immunoreaction was enhanced with tyramide signal amplification (1:50; Akoya Biosciences). Next ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... The procedure for multiplex immunohistochemistry (mIHC) was followed by a manufacturer’s protocol for Opal7-color automation IHC kit (Akoya Bioscience), and the staining was performed with Autostainer DISCOVERY ULTRA (Ventana) ...
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bioRxiv - Neuroscience 2022Quote: ... Bound probes were detected using the RNAscope v2 multiplex fluorescent detection kit and Opal 520 and 690 Dyes (Akoya Biosciences).
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bioRxiv - Immunology 2022Quote: Oligonucleotide barcoded antibody staining of tissue sections mounted on cover slips (n=1 section per tissue region per donor) was performed using a commercially available CODEX Staining Kit according to the manufacturer’s instructions for FFPE tissue (Akoya Biosciences) and as recorded for HuBMAP Lymphatic System TMC SOPs (https://www.protocols.io/view/codex-antibody-staining-protocol-for-ffpe-tissues-bbsdina6) ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the DIG-labeled probes were amplified with the Cy3 TSA reagent included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... the embryos were washed for five minutes in 500 μL of the 1X amplification diluent included in the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by streptavidin-conjugated peroxidase incubation and signal amplification using the TSA Plus Cyanine-3 or -5 kits (Akoya Biosciences).
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAPI (all nuclei) according to the manufacturer’s instructions (Opal 6-Plex Manual Detection Kit - for Whole Slide Imaging, NEL861001KT, Akoya Biosciences) (Supplementary Table 1) ...
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bioRxiv - Developmental Biology 2021Quote: ... FISH was performed as described (Brend and Holley 2009; Marra et al., 2017) using TSA Plus Fluorescein or Cyanine Kits (Akoya Biosciences). For all gene expression studies ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent RNA in situ hybridization was done with DIG-labeled probes for white and yellow with TSA Plus Fluorescence Kits (Akoya Biosciences) and used for in situ hybridization to imaginal discs as described (Tian et al ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Immunology 2024Quote: Sections of gliomas comprising B cell aggregates of at least 50 cells (n=33) were stained using the Opal Polaris 7-color manual detection kit (#NEL861001KT, Akoya Biosciences), based on the tyramide signal amplification immunostaining technique ...
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bioRxiv - Neuroscience 2020Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... The C4 probe complexes were first incubated with TSA–Biotin (Akoya NEL700A001KT, 1:250) for 30 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... for 12 minutes and with Opal 780 reactive fluorophore for 1 hour (Akoya Biosciences). Before each round of staining ...
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bioRxiv - Neuroscience 2021Quote: ... fos mRNA was then tagged to the fluorophore fluorescein (1:1500; NEL741E001KT, Akoya Biosciences), and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500 ...
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bioRxiv - Neuroscience 2021Quote: ... and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500; NEL745E001KT, Akoya Biosciences). Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were treated with TSA-plus Cyanine 3 (NEL744001KT; Akoya Biosciences; 1:1500). Fluoromount (K024 ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Neuroscience 2023Quote: ... Cy3 or fluorescein at 1:1000 in TSA buffer (TSATM Plus System, Akoya Bioosciences). Following that slides were incubated in HRP blocker for 15 min and then rinsed 3 times with wash buffer reagent ...
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bioRxiv - Neuroscience 2023Quote: ... HRP-C2 and HRP-C3 were labelled with Opal 520 (1:750, Akoya FP1487001KT) and Opal 570 (1:1500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were developed with Opal fluorescent dye at 1:50 dilution (Akoya Biosciences) in different channels ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in Opal 690 reagent for 15 minutes (Akoya Biosciences, 1:100) in blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... All Opal reagents were diluted 1:150 in an amplification buffer (Akoya Biosciences, FP1609) except Opal 780 ...