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Citations for Akoya Biosciences :
151 - 200 of 229 citations for 7 methoxy 4 piperidin 1 ium 1 ylmethyl 3 4 dihydro 2H 1 benzoxepin 5 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Immunofluorescence was visualized using Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in a Plus Automation Amplification Diluent (Akoya biosciences). Serial multiplexing was performed by repeating the sequence of antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... 690 fluorophores according to the protocol using 1:750 dilution of each fluorophore (Cat #FP1488001, #FP1497001, #FP1501001, Akoya Biosciences, Marlborough, MA). Slides were then mounted using Prolong Gold anti-Fade mounting media with DAPI and imaged at 600X magnification using the Nikon Ti2-E inverted microscope system (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Neuroscience 2023Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was washed several more times in TNT and DIG detection was performed using TSA Plus Cyanine diluted 1:50 in amplification diluent (NEL744001KT, Akoya Biosciences). Fluorescently labeled samples were imaged using confocal microscopy.
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Genomics 2024Quote: ... Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in 1x Plus Automation Amplification Diluent (Akoya biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... Probe remaining on the tissue sections was amplified and labeled with a single Opal 520 fluorophore at a dilution of 1:750 (OP-001001, Akoya Biosciences, Marlborough). After RNA labeling ...
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bioRxiv - Neuroscience 2023Quote: ... sections underwent signal amplification steps and fluorophore (1:750 dilutions of Opal™ Dyes: 520 cat# FP1487001KT, 570 cat# 1488001KT, 690 cat# 1497001KT; Akoya Biosciences) treatments according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Neuroscience 2021Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...
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bioRxiv - Pathology 2019Quote: The Vectra 3 platform (Akoya Biosciences, Hopkinton, MA), included inForm software ...
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bioRxiv - Cell Biology 2022Quote: ... and TSA-Cyanine 3 Plus (AKOYA, SKU NEL744001KT). RNAscope® 3-plex Negative Control Probe (Bacillus subtilis dihydrodipicolinate reductase (dapB ...
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bioRxiv - Neuroscience 2021Quote: ... The embryos are incubated with the 1:1,000 anti-digoxigenin-POD antibody overnight at 4°C and amplified with Tyramide Amplification working solution and exposed with fluorescein (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescein) (Akoya Biosciences, Marlborough, MA). All FISH embryos were mounted using DAPI in MOPs buffer and NucBlue (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hour at room temperature and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biosciences, Marlborough, MA). Cells were mounted in VectaShield Anti-Fade mounting media with DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biociences, Marlborough, MA). The embryos were washed with MOPS buffer three times then with PBST (1xPBS,0.1% TritonX-100 ...
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bioRxiv - Neuroscience 2023Quote: ... The signal was developed using the HRP for each channel and then opal dyes diluted at 1:1000 were assigned for each channel: tdTomato was assigned to Opal 690 (Akoya Bioscience, catalog #FP1497001KT), GluN2C was assigned to Opal 570 (Akoya Bioscience ...
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bioRxiv - Genomics 2023Quote: ... three signal amplification steps were performed and HRP signal was developed using Opal™ 570 at 1:1500 dilution (Akoya Biosciences, Cat. FP1488001KT). IF was performed after the HRP blocker step with all steps conducted in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... The green and red dyes were prepared at a concentration of 1:1000 by combining 1000 µl of TSA Buffer (Advanced Cell Diagnostics, 322809) with 1 µl of Opal 520 Reagent (in DMSO, Akoya Biosciences, OP-001001) and Opal 570 Reagent (in DMSO ...
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bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Signals were amplified by TSA-plus Cyanine 3 (AKOYA Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... Signals were amplified by TSA-plus Cyanine 3 (AKOYA Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... an Opal 3-Plex Manual Detection Kit (Akoya Biosciences) was used following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Signals were amplified by TSA-plus Cyanine 3 (AKOYA Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Kcnv1 mRNA was detected using the target probe Mm-Kcnv1 (Advanced Cell Diagnostics) and the Opal™ 650 dye (1:1500, Akoya Biosciences, Marlborough, MA, USA)
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Neuroscience 2023Quote: ... Signals were amplified by TSA-plus Cyanine 3 (NEL744001KT, Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... a TSA Plus Cyanine 3 System (Akoya Biosciences, Marlborough, MA,) was used for rabbit primary antibody treated sets following instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were scanned with the Vectra® 3 (Akoya Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2020Quote: ... Multiplex IHC slides were imaged on a Vectra 3 (Akoya Biosciences). Spectral unmixing and cell phenotype identification was performed using inForm software (Akoya Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Developmental Biology 2021Quote: ... TSA Plus Cyanine 5 Kit (Akoya Biosciences, NEL745001KT) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... a 5 min DAPI staining (Akoya Biosciences, #FP1490) was performed before mounting with ProLong Diamond Antifade media (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sections were subsequently incubated in TSA-plus Cyanine 3 (#NEL744001KT, AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Microbiology 2022Quote: ... and TSA Plus-Cyanine 5 (AKOYA Biosciences, cat# NEL745001KT), respectively ...
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bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...
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bioRxiv - Developmental Biology 2021Quote: ... Chromogenic reaction was performed with either TSA Plus Cyanine 3 Kit (Akoya Biosciences) for nkx3.1 or ImmPACT Vector Red (Vector Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Multispectral images were acquired using a Vectra® 3 imaging system (Akoya Biosciences). The QuPath software (version 0.3.2 ...
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bioRxiv - Neuroscience 2023Quote: ... and TACR1 (Cat 310701-3, ACD) paired with Cy5 (Cat NEL745001KT, Akoya Biosciences). The third channel (green ...