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Citations for Akoya Biosciences :
151 - 191 of 191 citations for 7 Amino 4 methyl 1 8 naphthyridin 2 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Immunology 2023Quote: ... Counterstaining was performed with DAPI for 5 min at room temperature (1 drop per 500 µL, Akoya Bioscience). Microscope images were taken using the Zeiss AxioImager M2 and analyzed using ZEN blue software (v2.3).
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bioRxiv - Cancer Biology 2021Quote: Multispectral analysis of FFPE tissue utilised the MOTiF™ PD-1/PD-L1 kit (Akoya Biosciences, cat# OP-000001). Staining with Leica BOND RX (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:200 in TNB blocking buffer) for 45 min followed by DAPI staining for 5 min (Spectral DAPI, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorophores applied to detect the signal from the probes were Opal 690 Reagent (1:750; Akoya Biosciences, #FP1497001KT) for C1 and Opal 570 Reagent (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Cell Biology 2021Quote: ... For amplified targets the appropriate HRP-conjugated secondary was then incubated at 1:250 in PBS for 45 minutes-1hr followed by TSA fluorophore treatment (Akoya). For unamplified targets appropriate Cy3 or FITC labeled secondary antibodies were used at 1:100 ...
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bioRxiv - Immunology 2020Quote: We stained 5-µm-thick formalin-fixed paraffin-embedded sections from human xenograft tumors (UMSCC-1) using Opal multiplex kits (PerkinElmer/Akoya), for a panel of DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated in Opal 520 diluted in blocking buffer (for CK19 and CD8 primary) (Akoya Biosciences, 1:100) for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:500 in the same blocking buffer that is included with the TSA Signal Amplification kit from Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... ISH was completed by washing slides 3x for 15 minutes in PBS before incubation in Polaris 780 (1:125 in diluent buffer, Akoya) for 30 minutes at 40C ...
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bioRxiv - Neuroscience 2023Quote: ... Final detection was achieved with the Opal-570 and Opal-690 fluorophore reagent packs (Akoya BioSciences, Inc.,diluted 1:1000), and samples were conterstained with DAPI (ACD ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (1:1000) were assigned for each channel: Vgat expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), Npy expression was identified by Opal 570 (Akoya Bioscience ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... a working Opal dye solution was made fresh using a 1:500 ratio of Opal dye (Akoya Biosciences, Marlborough, MA, USA) to TSA buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... then covered by 80 μL of Opal 520 at 1/1500 (Opal 520 reagent pack, FP1487001KT, AKOYA Biosciences, Malborough, United States) during 30 min at 40°C and washed at RT with RNAscope wash buffer two times ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were further incubated with 1:200 TSA Plus FITC or Cy3 reagents for 3-5 minutes of signal development (NEL774001KT or NEL771B001KT, Akoya Bioscience). Sections were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunofluorescence was visualized using Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in a Plus Automation Amplification Diluent (Akoya biosciences). Serial multiplexing was performed by repeating the sequence of antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... 690 fluorophores according to the protocol using 1:750 dilution of each fluorophore (Cat #FP1488001, #FP1497001, #FP1501001, Akoya Biosciences, Marlborough, MA). Slides were then mounted using Prolong Gold anti-Fade mounting media with DAPI and imaged at 600X magnification using the Nikon Ti2-E inverted microscope system (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Neuroscience 2023Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was washed several more times in TNT and DIG detection was performed using TSA Plus Cyanine diluted 1:50 in amplification diluent (NEL744001KT, Akoya Biosciences). Fluorescently labeled samples were imaged using confocal microscopy.
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Genomics 2024Quote: ... Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520, 540, 570, 620, 650 and 690) diluted at 1:150 in 1x Plus Automation Amplification Diluent (Akoya biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... Probe remaining on the tissue sections was amplified and labeled with a single Opal 520 fluorophore at a dilution of 1:750 (OP-001001, Akoya Biosciences, Marlborough). After RNA labeling ...
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bioRxiv - Neuroscience 2023Quote: ... sections underwent signal amplification steps and fluorophore (1:750 dilutions of Opal™ Dyes: 520 cat# FP1487001KT, 570 cat# 1488001KT, 690 cat# 1497001KT; Akoya Biosciences) treatments according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Neuroscience 2021Quote: ... The embryos are incubated with the 1:1,000 anti-digoxigenin-POD antibody overnight at 4°C and amplified with Tyramide Amplification working solution and exposed with fluorescein (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescein) (Akoya Biosciences, Marlborough, MA). All FISH embryos were mounted using DAPI in MOPs buffer and NucBlue (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hour at room temperature and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biosciences, Marlborough, MA). Cells were mounted in VectaShield Anti-Fade mounting media with DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biociences, Marlborough, MA). The embryos were washed with MOPS buffer three times then with PBST (1xPBS,0.1% TritonX-100 ...
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bioRxiv - Neuroscience 2023Quote: ... The signal was developed using the HRP for each channel and then opal dyes diluted at 1:1000 were assigned for each channel: tdTomato was assigned to Opal 690 (Akoya Bioscience, catalog #FP1497001KT), GluN2C was assigned to Opal 570 (Akoya Bioscience ...
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bioRxiv - Genomics 2023Quote: ... three signal amplification steps were performed and HRP signal was developed using Opal™ 570 at 1:1500 dilution (Akoya Biosciences, Cat. FP1488001KT). IF was performed after the HRP blocker step with all steps conducted in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... The green and red dyes were prepared at a concentration of 1:1000 by combining 1000 µl of TSA Buffer (Advanced Cell Diagnostics, 322809) with 1 µl of Opal 520 Reagent (in DMSO, Akoya Biosciences, OP-001001) and Opal 570 Reagent (in DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... Signals were amplified using biotin/streptavidin HRP-conjugated secondary Ab (Jackson Immuno, 111-065-003 and 016-030-084) and detected using Tyramide Signal Amplification Plus kit (Akoya Biosciences, NEL744001KT, 1:100). Images were taken using a Leica SP5X laser scanning confocal microscope and further processed using ImageJ Fiji software (Schindelin et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Kcnv1 mRNA was detected using the target probe Mm-Kcnv1 (Advanced Cell Diagnostics) and the Opal™ 650 dye (1:1500, Akoya Biosciences, Marlborough, MA, USA)