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Citations for Akoya Biosciences :
101 - 140 of 140 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Multiplex immunofluorescence (mIF) analysis was performed with tyramide signal amplification using Opal fluorescent dyes (Akoya Biosciences). Three mIF panels were used in this study (IP1.2 ...
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bioRxiv - Genomics 2020Quote: ... Fluorescent labeling was conducted using Opal 480 (GFAP) and Opal 570 (CD44) dyes (1:150; Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories) with TSA with Opal dyes (Akoya Biotech). Sections were mounted in Mowiol for microscopy (Roth ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromogenic detection of probes was performed using OpalTM 520 (FP1487001KT) and OpalTM 570 (FP1488001KT) dyes (Akoya Biosciences) and Hoechst 33358 (1:1500 ...
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bioRxiv - Neuroscience 2023Quote: ... Pv-positive interneurons were stained using a Pvalb probe (#421931-C2) developed using Opal dye 620 (Akoya Biosciences ...
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bioRxiv - Physiology 2024Quote: ... 30 min incubation with a tyramide dye fluorophore (Opal 620, Cat. No: FP1495001KT, Akoya Biosciences, Marlborough, USA) diluted 1:1000 in RNAscope TSA dilution buffer (ACD Bio) ...
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bioRxiv - Cell Biology 2024Quote: ... and myoD1/2 (condition 24h) was detected using the fluorescent dyes Opal 620 (#OP-001004, Akoya Biosciences). For the cells under the 72h condition ...
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bioRxiv - Neuroscience 2024Quote: ... Advanced Cell Diagnostics) and labeled using 1:1500 diluted Opal 570 and 690 dyes respectively (Akoya Biosciences). Labeled sections were stained with DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... Hybridisation was done on 6 µm formalin fixed paraffin sections and detected using Opal dyes (Akoya Biosciences) (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... Hybridized slides were incubated with amplification reagents and Opal 570 and 690 dyes (Akoya Biosciences, 1:1500 dilution) followed by sequential incubation with tyrosine hydroxylase (Aves Labs ...
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bioRxiv - Immunology 2024Quote: ... The detection of the target proteins was achieved using optimized fluorescent Opal tyramide signal amplification (TSA) dyes (Akoya), along with repeated cycles of antibody denaturation ...
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bioRxiv - Neuroscience 2023Quote: ... CA) using probes against the leptin receptor mRNA (Cat# 415951) and Opal 650 dye kit (Akoya Biosciences, Marlborough, MA) to label LepR mRNA ...
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bioRxiv - Pathology 2024Quote: ... the respective Opal dye (Opal Polaris 480 reagent pack, FP1501001KT, Akoya Biosciences; Opal Polaris 570 reagent pack, FP1488001KT, Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Multispectral images were unmixed using a spectral library built from images of single stained control tissues for each OPAL dye using the inForm Advanced Image Analysis software (inForm 2.8; Akoya). A selection of 10-15 representative multispectral images spanning all tissue sections was used to train the Qupath software (tissue segmentation ...
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bioRxiv - Neuroscience 2024Quote: ... and opal dyes (520 nm for channel 1; 570 nm for channel 2; 690 nm for channel 3; Akoya Biosciences ...
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bioRxiv - Genomics 2024Quote: ... Probe channels were developed using TSA-Opal dyes (Opal 520, Opal 570 and Opal 650; 1:1000; Akoya Biosciences), TSA-biotin (TSA Plus Biotin Kit ...
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bioRxiv - Genetics 2024Quote: ... and opal dyes (520 nm for channel 1; 570 nm for channel 2; 690 nm for channel 3; Akoya Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... Bound probes were detected using the RNAscope v2 multiplex fluorescent detection kit and Opal 520 and 690 Dyes (Akoya Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (1:1000) were assigned for each channel: Vgat expression was identified by Opal 520 (Akoya Bioscience, catalog # FP1487001KT), Npy expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... CasL/NEDD9 (ACD, 446311), and Sin/EFS (ACD, 806951) probes generated by Advanced Cell Diagnostics were uniquely amplified with Opal dyes (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Three amplification steps were carried out prior to developing the signal with Opal™ or TSA® Dyes (Akoya Biosciences). Immunostaining following the RNAscope® experiment was performed in some experiments according to Technical Note 323100-TNS from ACDBio ...
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bioRxiv - Neuroscience 2021Quote: In situ hybridization was performed with the RNAscope® Multiplex Fluorescent Assay v2 from Advanced Cell Diagnostics (ACD) combined with Tyramide Signal Amplification technology (TSA™) and dyes from Akoya Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... followed by sequential detection of each probe [15 minutes at 40°C with probe-specific HRP then 30 minutes at 40°C with Opal dye (1:500; C1:Opal 570, C2: Opal 620, C3: Opal 520; Akoya Biosciences)] ...
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bioRxiv - Genomics 2024Quote: ... Each cycle used a different fluorescent Opal dye (520, 570, 620, or 690; catalog no. FP1487001KT, FP1488001KT, FP1495001KT, and FP1497001KT, respectively; Akoya Biosciences) diluted 1:500 in TSA buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Probe sets for Trem2 (ACD 404111-C2) and Tmem119 (ACD 472901-C3) were used with Opal 520 and Opal 570 dyes (Akoya Biosciences FP1487001KT), respectively ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Neuroscience 2023Quote: ... sections underwent signal amplification steps and fluorophore (1:750 dilutions of Opal™ Dyes: 520 cat# FP1487001KT, 570 cat# 1488001KT, 690 cat# 1497001KT; Akoya Biosciences) treatments according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Opal dyes (1:1000) were assigned for each channel: Assay # 1: tdTomato expression was identified by Opal 690 (Akoya Bioscience, catalog # FP1497001KT), Vglut2 expression was identified by Opal 570 (Akoya Bioscience ...
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bioRxiv - Immunology 2023Quote: ... HRP-C1 was applied for 15 min at 40°C after which the slides were washed in wash buffer and incubated with Opal dye 570 (Akoya, OP-001003) diluted 1:1500 in TSA buffer (ACD ...
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bioRxiv - Neuroscience 2024Quote: ... probe hybridization and signal amplification were carried out according to RNAscope™ Multiplex Fluorescent V2 Assay using Lgr5-C1 probe and Opal dye 570 (Akoya Sciences). Slides were mounted with Vectashield and DAPI.
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bioRxiv - Neuroscience 2024Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Amplifiers were added using the proprietary AMP reagents and the signal was visualized through probe-specific HRP-based detection by tyramide signal amplification (TSA) with Opal dyes Opal 520 and Opal 570 (FP1487001KT and FP1488001KT; Akoya Biosciences, United States) diluted to 1:700 ...
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bioRxiv - Neuroscience 2024Quote: ... The signal was developed using the HRP for each channel and then opal dyes diluted at 1:1000 were assigned for each channel: tdTomato was assigned to Opal 690 (Akoya Bioscience, catalog # FP1497001KT), Glun2c was assigned to Opal 570 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Detection of mRNA was achieved using Opal™ fluorophore dyes from the 520 (1:500) and 620 (1:750) reagent packs (Akoya Biosciences, USA). Full protocol details are available in Supplementary Methods ...
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bioRxiv - Neuroscience 2024Quote: ... were applied and amplified in sequence with TSA Vivid and Opal Polaris dyes (Advanced Cell Diagnostics, cat# 323271, 323272, 323273; Akoya Biosciences, cat# FP1501001KT). Cellular nuclei were counterstained with 1 µg/ml DAPI and mounted with Prolong Glass Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Fluorescent labelling of the RNAscope® probes was achieved by using OPAL 520 and OPAL 570 dyes (Cat. No. FP1487001KT + Cat. No. FP1488001KT, Akoya Biosciences, Marlborough, MA, USA) and stained sections were scanned at 25x magnification using a LSM 980 with Airyscan 2 (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2024Quote: ... Kcnv1 mRNA was detected using the target probe Mm-Kcnv1 (Advanced Cell Diagnostics) and the Opal™ 650 dye (1:1500, Akoya Biosciences, Marlborough, MA, USA)