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Citations for Akoya Biosciences :
101 - 119 of 119 citations for Coatomer Subunit Epsilon COPE Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by re-hydration in PBS for 5 minutes at room temperature and blocking with 1x Antibody diluent/Block solution (Akoya Biosciences ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Cancer Biology 2021Quote: ... was used for antibody raised in rabbit (PH3 and PHGDH) and the OPAL Polymer HRP Ms+Rb (Akoya/Perkin Elmer, ARH1001EA) was used for the antibody raised in mouse (panCK) ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with HRP-conjugated anti-DIG (1:500) antibody overnight and treated with TSA-plus biotin (NEL749A001KT; Akoya Biosciences), followed by streptavidin-Alexa Fluor 488 (S32354 ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Neuroscience 2024Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Microbiology 2024Quote: ... followed by an anti-mouse secondary antibody conjugated to HRP and visualized with Opal 690 (cat#OP-001006, Akoya, Marlborough, MA). CD34 was quenched using the peroxide block ...
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bioRxiv - Cancer Biology 2024Quote: The slides were then incubated for 10 minutes with the horseradish peroxidase-conjugated secondary antibodies Opal polymer HRP mouse-rabbit (Akoya Biosciences), ImmPRESS® HRP Goat Anti-Rat IgG HRP-polymer or ImmPRESS® HRP Goat Anti-Rabbit IgG HRP-polymer (Vector Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... the HRP-coupled secondary anti-rat OmniMap antibody (Ventana Medical Systems) for 12 min and finally Opal 520 fluorophore (Akoya Biosciences, 1:100) at RT for 8 min ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...