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Citations for Akoya Biosciences :
101 - 150 of 245 citations for 6 methyl 5 6 7 8 tetrahydro 1 3 dioxolo 4 5 g isoquinolin 6 iumchloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The reduction solution was removed by centrifugation (12,000 x g) and exchanged with the conjugation solution (Akoya Biosciences, 7000009). Each oligonucleotide barcode was rehydrated in 10 µl nuclease-free water (Life Technologies ...
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bioRxiv - Genomics 2021Quote: Tissue samples stained with mIHC were scanned with multispectral imaging microscopy (Vectra 3, Akoya Bioscience). Scanned multispectral images were unmixed on inForm software (ver.2.4.0 ...
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bioRxiv - Cancer Biology 2022Quote: The stained slides were scanned on a Perkin Elmer Vectra 3 imaging system (Akoya Biosciences) and analyzed using Halo Image Analysis platform (Indica Labs) ...
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bioRxiv - Neuroscience 2024Quote: ... sections following HPR-C1 treatment were incubated with TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
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bioRxiv - Cancer Biology 2023Quote: Seven color multiplex-stained slides were imaged using the Vectra Multispectral Imaging System version 3 (Akoya). Scanning was performed at 20x (200x final magnification) ...
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bioRxiv - Molecular Biology 2023Quote: ... the development reaction was carried out using the TSA Plus Cyanine 3 Kit (Akoya Biosciences, US) (FISH ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed and analysed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Developmental Biology 2024Quote: ... after which the second color was generated by Cyanine 3 incubation (NEL744001KT, Akoya biosciences, Marlborough, MA, USA) for five minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were imaged at both 10x and 20x using the Vectra 3 Polaris and Vectra imaging software (Akoya Biosciences). Acquired qpTIFF images from the Vectra Polaris system were imported into QuPath analysis software(84 ...
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bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... visualization of 4-color opal slides was performed by Vectra Polaris Quantitative Pathology Imaging Systems (Akoya, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Immunology 2021Quote: ... Multispectral images were acquired using a Vectra® 3 imaging system employing the Vectra3 and Phenochart software (all Akoya Biosciences). Images are displayed in pseudocolours ...
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 3 times in PBS and incubated in final fixative solution (1000uL of PBS + 20uL of Akoya’s final fixation reagent) for 20 minutes at room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... of HNSCC tumors were sectioned to 4-μm thick for the subsequent multiplex immunohistochemistry via the OPAL Polaris system (Akoya Biosciences). After deparaffinization and hydration ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence staining of AKPT tumours was performed on 4 μm thick FFPE sections using the OPAL protocol (Akoya Biosciences, Marlborough, MA). The Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Multiplex immunofluorescence (MPIF) staining was performed on formalin-fixed paraffin embedded (FFPE) sections of thickness 4-µm using the OPAL protocol (Akoya Biosciences, Marlborough, MA) on the Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2021Quote: ... Opal™ 570 (Akoya; #FP1488001KT; 1:1500) was applied for channel 1 and Opal™ 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 690 dyes (1:1000, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...