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Citations for Akoya Biosciences :
51 - 100 of 151 citations for Ubiquitin thioesterase ZRANB1 ZRANB1 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... sections were incubated in HRP-C2 at 40°C for 15mins followed by Opal650 (Akoya) in TSAP diluent for 30mins at 40°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the same steps were repeated with HRP-C2 solution and OPAL flurophore 570 (Akoya Biosciences). After nuclei staining with DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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bioRxiv - Pathology 2021Quote: ... or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories) with TSA with Opal dyes (Akoya Biotech). Sections were mounted in Mowiol for microscopy (Roth ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies were diluted using antibody diluent (Akoya Biosciences, Marlborough, USA) and incubated for 45 min at RT followed by 3x 3 min washing with 1x TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse and anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) were then applied as a secondary label for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse plus anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) was applied as a secondary label for a 10 minute incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Immunology 2022Quote: ... several steps are carried out in order to amplify the IL-22BP signal: polymer enhancer (M023 from ImPath) then HRP-2 (M024 from ImPath) and finally Opal 650 (FP1496A from Akoya) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... then with the EnVision+ System-HRP Labelled Polymer Anti-Rabbit and finally with OPAL-670 diluted in amplifier diluent (Akoya Biosciences). The procedure was repeated for αCD8 (4SM15 ...
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bioRxiv - Neuroscience 2023Quote: ... we developed the Epor signal incubating the samples with HRP-C1 (30 min., CatNo. 323104, ACD) and Opal 570 (30 min., CatNo. FP1487001KT, Akoya biosciences), followed by the blockade of the horseradish peroxidase action using HRP-blocker (CatNo ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification steps were performed as instructed by manufacturer and signal was developed using HRP-C1 followed by incubation with Opal 570 (Akoya FP1488001KT) with the following dilutions ...
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bioRxiv - Immunology 2024Quote: ... the appropriate heat induced epitope retrieval for all the targets was performed using the appropriate buffer (AR6 or AR9) for 15 minutes followed by incubation with Opal Polymer HRP Ms + Rb (Akoya Biosciences). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were then incubated with a 1:200 dilution of Opal Polymer anti-Rabbit HRP (Akoya Biosciences, Marlborough, MA, USA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Identified antibody clones were then conjugated with oligonucleotide barcodes using Antibody Conjugation Kit (Akoya Biosciences). Prior to CODEX imaging ...
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bioRxiv - Microbiology 2024Quote: ... was incubated at ambient temperature for 30 min and detected with HRP-conjugated goat anti-rat and visualized with Opal 620 (cat#OP-001004, Akoya, Marlborough, MA). The LANA signal was quenched using the Leica kit’s peroxide block ...
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bioRxiv - Cell Biology 2021Quote: Antibodies were purchased preconjugated from Akoya Biosciences or sourced from other vendors and conjugated in-house using the CODEX Conjugation Kit (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Antibody conjugation requires reagents from Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Genomics 2023Quote: ... three signal amplification steps were performed and HRP signal was developed using Opal™ 570 at 1:1500 dilution (Akoya Biosciences, Cat. FP1488001KT). IF was performed after the HRP blocker step with all steps conducted in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... sections were then incubated with HRP-channel of gene 1 for 15 min at 40°C and Opal 620 (1:1500; Akoya, Biosciences, Cat# FP1495001KT) diluted in TSA buffer (Akoya ...
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bioRxiv - Neuroscience 2024Quote: ... Amplifiers were added using the proprietary AMP reagents and the signal was visualized through probe-specific HRP-based detection by tyramide signal amplification (TSA) with Opal dyes Opal 520 and Opal 570 (FP1487001KT and FP1488001KT; Akoya Biosciences, United States) diluted to 1:700 ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...
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bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... diluted in Antibody Diluent/Block (Akoya, ARD1001EA) for 30min at RT ...
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bioRxiv - Immunology 2024Quote: ... incubated with blocking antibody diluent (Akoya, ARD1001EA) for 10 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... Oligo-conjugated primary antibodies were prepared 50µg at a time using the Akoya Antibody Conjugation Kit (Akoya Biosciences, Delaware, USA) according to the manufacturer’s recommended protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 minute incubation with the secondary antibody (Akoya), 10 minute incubation with the respective OPAL (Akoya ...
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bioRxiv - Immunology 2024Quote: ... 1X Antibody Diluent/Block solution (ARD1001EA, Akoya Biosciences) was used ...
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bioRxiv - Genomics 2021Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Slides were counter stained with DAPI ...
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bioRxiv - Genomics 2024Quote: ... Slides were incubated for 1 hour at room temperature with primary antibodies diluted in 1x Opal blocking/antibody diluent (Akoya biosciences). Slides were subsequently incubated with the Opal Polymer HRP Ms + Rb secondary polymer for 30 minutes prior to incubation with Opal fluorophores (Opal 520 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were incubated in primary antibody overnight and secondary antibody conjugated to peroxidase and then developed using Tyramide Signal Amplification (TSA, Akoya Biosciences). Samples were stained with either hematoxylin and eosin or the following antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide prior to the addition of any primary antibodies which were prepared in the Opal Blocking/Antibody Diluent (Akoya biosciences). A pan-immune antibody panel and a macrophage subset antibody panel were used to investigate immune cell localization in brain metastasis samples ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... slides were blocked with 1X antibody diluent/block (Akoya Biosciences) for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies were coupled to OPAL520 (AKOYA Biosciences Cat# FP1487001KT), OPAL570 (AKOYA Biosciences Cat#FP1488001KT) ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...