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Citations for Akoya Biosciences :
51 - 100 of 100 citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 DbpA Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 1X Antibody Diluent/Block (Akoya Biosciences) for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected with OPAL Polymer HRP Ms + Rb (Akoya Biosciences) for 15 minutes and visualized following a 10-minute incubation with Tyramide signal amplification and Opal Fluorophore (Akoya ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were diluted in 1X Plus Automation Amplification Diluent (Akoya Biosciences). Primaries were detected using according anti-species secondary antibodies conjugated to HRP (OmniMap Ventana ...
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bioRxiv - Cancer Biology 2024Quote: ... Scanning of the full antibody panel was performed on PhenoImager (Akoya Biosciences). A spectral fluorophore for each primary antibody and autofluorescence library was made to enable optimal multispectral unmixing ...
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bioRxiv - Neuroscience 2024Quote: ... after which the hybridized probes were detected using peroxidase conjugated anti-FLU or anti-DIG Fab(2) fragments and the Tyramide signal amplification (TSA) system from Akoya Biosciences ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Secondary antibodies were obtained from Akoya (OPAL 690, OPAL 520, and OPAL 570) and diluted to a concentration of 1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... panCK staining was performed by using an OPAL TSA-DIG antibody (Akoya Biosciences) in combination with an OPAL 780 fluorophore conjugated anti-DIG antibody ...
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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bioRxiv - Pathology 2024Quote: ... To validate the efficacy of commercial DNA-barcode-conjugated antibodies for PhenoCycler by Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... The tissue section was stained with a cocktail of DNA-barcoded antibodies purchased from Akoya company (CD4/EPR6855 ...
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bioRxiv - Bioengineering 2023Quote: The antibody conjugation and the staining were done according to the manufacturer’s protocol (Akoya Biosciences). Details regarding primary antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal for antibody complexes was labeled and visualized by their corresponding Opal Fluorophore Reagents (Akoya) by incubating the slides for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 5 (1:300 dilution) with Opal 690 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Opal Anti-Ms + Rb HRP (Akoya Biosciences, Marlborough, MA ARH1001EA), before conjugating with Opal fluorophores Following incubation ...
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bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 10-minute incubation with Opal Antibody Dilutent/Block solution (Akoya Bioscience, Marlborough, USA). The first primary antibody was then applied overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... RNA probes and panCK antibody were detected with the following Opal Fluorophores pairings: CXCL13 Opal 480 (Akoya FP1500001KT), CXCL10/11 Opal 520 (Akoya FP1487001KT) ...
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bioRxiv - Immunology 2022Quote: ... Each target was linked using the OPAL Polymer HRP conjugated secondary antibodies (ready-to-use, ARH1001EA, AKOYA Biosciences). Visualization of the different targets was established using the OPAL Polaris 570 (FP1488001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed in TBS + 0.1% tween (TBS-T) and blocked in Antibody Diluent/Block (Akoya Biosciences, ARD1001EA) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of probe and antibody were performed using Tyramide Signal Amplification system with Opal 570 or 520 reagents (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Then a staining dye was added after secondary antibody washes: α-SMA – Opal-dye 520 (Akoya Biosciences OP-001001) at 1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Genomics 2022Quote: A total of 32 antibodies were used for CODEX and 19 of them were conjugated in-house using a protocol outlined by Akoya Biosciences (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: FFPE tissue sections containing OB and OT were stained consecutively with specific primary antibodies according to standard protocols provided by Akoya and performed routinely by the HIMSR ...
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bioRxiv - Pathology 2023Quote: ... secondary antibodies labeled with horseradish peroxidase (all with reduced cross-reactivity from Jackson-Immunoresearch) in combination with 7plex Opal system (Akoya) were used according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional antibodies were obtained from commercial vendors as indicated and conjugated to custom oligo barcodes according to instructions in Akoya’s antibody conjugation kit (Conjugation kit, Akoya, #7000009). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table S5) according to Akoya’s PhenoCycler-Fusion user guide using the antibody conjugation kit (Akoya, 7000009) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies (described above for western blotting ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then blocked with 10% BSA + 0.05% Tween20 and the antibodies were diluted in 2% BSA in 0.05% PBST and used in a multiplexed manner with OPAL reagents (Akoya Biosciences). All primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Pathology 2024Quote: ... we stained antibodies on tonsil tissue and aorta tissue microarray using the sample kit for PhenoCycler-Fusion (Akoya Biosciences, 7000017) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Antigen retrieval and blocking were performed on the dewaxed slides using the Antigen retrieval reagent (pH 6.0) and Blocking/antibody diluent provided in the Opal Polaris Multicolor Manual IHC Detection Kit (Akoya Biosciences, USA), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... polymer-conjugated secondary antibody for 20 min and reacted with one of the tyramide signal amplification reagents including Opal 520 (Akoya, FP1487001KT), Opal 570 (Akoya ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Developmental Biology 2023Quote: All fixed frozen tissue CODEX antibody stainings were done according to Akoya Biosciences staining protocol associated with the CODEX Staining Kit with some modifications (Akoya; 7000008). After tissue hydration ...
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bioRxiv - Cell Biology 2023Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies (1:100) followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...