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Citations for Akoya Biosciences :
51 - 100 of 178 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Slides were stained for markers using the 7-plex Opal Polaris kit (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by Opal Polymer HRP Ms + Rb (Akoya Biosciences, cat # ARH1001EA) and tyramide-linked 650 Opal fluorophore (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... slides were incubated with opal polymer HRP ms + rab (Akoya biosciences) or HRP-goat (GBI ...
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bioRxiv - Immunology 2024Quote: ... we utilized a commercially available manual mIF staining kit (Opal 7-plex, Akoya, Cat# NEL811001KT) and applied an optimized protocol to all mIF staining (Supplementary Table S2) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were washed and underwent Opal HRP polymer incubation (Akoya Biosciences, ARH1000EA) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... detection was performed using a secondary horseradish peroxidase (HRP)-conjugated polymer (Akoya Opal polymer HRP Ms + Rb ARH1001 ...
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bioRxiv - Physiology 2023Quote: ... The sections were then incubated for 30 minutes in streptavidin HRP (Akoya Biosciences #TS-000300 ...
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bioRxiv - Cancer Biology 2023Quote: ... detection was performed using a secondary horseradish peroxidase (HRP)- conjugated polymer (Akoya Opal polymer HRP Ms + Rb ARH1001EA ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were repeated with HRP-C2 and Opal 690 (Akoya Biosciences), then with HRP-C3 and CF405M (Biotium) ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated with Opal Anti-Ms + Rb HRP (Akoya Biosciences ARH1001EA) for 20 min ...
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bioRxiv - Immunology 2024Quote: ... incubated with Opal Polymer HRP (anti-mouse and anti-rabbit, Akoya, ARH1001EA) for 10 minutes and labeled with Opal dyes (Akoya ...
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 690 fluorophore (Akoya Biosciences, 1:50). Finally ...
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bioRxiv - Immunology 2022Quote: ... with OmniMap anti-rabbit-HRP and Opal 570 fluorophore (Akoya Biosciences, 1:1000), and lastly CD3 (abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...
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bioRxiv - Immunology 2023Quote: ... The same steps were repeated for HRP-C2 with Opal 690 (Akoya, OP-001006) and HRP-C4 with Opal 520 (Akoya ...
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bioRxiv - Developmental Biology 2024Quote: ... This same process was repeated for HRP-C2 and OPAL 570 (Akoya Biosciences, FP1488001KT), and for HRP-C3 ...
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bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
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bioRxiv - Immunology 2024Quote: ... Staining was done following the guidelines in the Opal 7-color Manual IHC Kit (Akoya Biosciences, Catalog: NEL811001KT). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The staining for mIHC was carried out with the Opal Polaris 7 color IHC Detecton Kit (Akoya Biosciences, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated in HRP-C2 at 40°C for 15mins followed by Opal650 (Akoya) in TSAP diluent for 30mins at 40°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the same steps were repeated with HRP-C2 solution and OPAL flurophore 570 (Akoya Biosciences). After nuclei staining with DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies (including pre-conjugated antibodies from Akoya) were additionally validated on the respective GBM control samples using multiple CODEX runs to adjust antibody concentration and exposure times to optimize signal to noise ratio ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
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bioRxiv - Immunology 2024Quote: ... 7-plex stainings were acquired at 0.5 µm/pixel on the Vectra Polaris™ Automated Quantitative Pathology Imaging System (Akoya Biosciences) using MOTiF technology ...
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bioRxiv - Immunology 2024Quote: Sections of gliomas comprising B cell aggregates of at least 50 cells (n=33) were stained using the Opal Polaris 7-color manual detection kit (#NEL861001KT, Akoya Biosciences), based on the tyramide signal amplification immunostaining technique ...
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bioRxiv - Pathology 2021Quote: ... or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories) with TSA with Opal dyes (Akoya Biotech). Sections were mounted in Mowiol for microscopy (Roth ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies were diluted using antibody diluent (Akoya Biosciences, Marlborough, USA) and incubated for 45 min at RT followed by 3x 3 min washing with 1x TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse and anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) were then applied as a secondary label for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse plus anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) was applied as a secondary label for a 10 minute incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... antibodies were conjugated using Akoya Antibody Conjugation Kit (Akoya Biosciences, SKU 7000009) with a barcode (Akoya Biosciences ...
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bioRxiv - Immunology 2021Quote: The antibody panel was composed of commercially available AKOYA-conjugated antibodies and self-conjugated custom antibodies (CODEX Conjugation Kit, AKOYA Biosciences). Tissue staining was performed with the CODEX Staining Kit (AKOYA Biosciences) ...
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bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...
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bioRxiv - Neuroscience 2021Quote: ... the C4 probe signal was developed as above but with HRP-C4 and Opal 620 dye (Akoya Biosciences; 1:1500). Slides were coverslipped with ProLong Gold Antifade Mountant (Invitrogen™ ...
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bioRxiv - Immunology 2022Quote: ... several steps are carried out in order to amplify the IL-22BP signal: polymer enhancer (M023 from ImPath) then HRP-2 (M024 from ImPath) and finally Opal 650 (FP1496A from Akoya) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... The slides were then washed in 1X TBS-T and incubated in HRP-conjugated goat-anti-rabbit secondary (1:1500, PerkinElmer NEF812001EA, Akoya Biosciences) for 45 minutes ...
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bioRxiv - Immunology 2021Quote: ... then with the EnVision+ System-HRP Labelled Polymer Anti-Rabbit and finally with OPAL-670 diluted in amplifier diluent (Akoya Biosciences). The procedure was repeated for αCD8 (4SM15 ...
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bioRxiv - Neuroscience 2023Quote: ... we developed the Epor signal incubating the samples with HRP-C1 (30 min., CatNo. 323104, ACD) and Opal 570 (30 min., CatNo. FP1487001KT, Akoya biosciences), followed by the blockade of the horseradish peroxidase action using HRP-blocker (CatNo ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification steps were performed as instructed by manufacturer and signal was developed using HRP-C1 followed by incubation with Opal 570 (Akoya FP1488001KT) with the following dilutions ...
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bioRxiv - Immunology 2024Quote: ... the appropriate heat induced epitope retrieval for all the targets was performed using the appropriate buffer (AR6 or AR9) for 15 minutes followed by incubation with Opal Polymer HRP Ms + Rb (Akoya Biosciences). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were then incubated with a 1:200 dilution of Opal Polymer anti-Rabbit HRP (Akoya Biosciences, Marlborough, MA, USA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Identified antibody clones were then conjugated with oligonucleotide barcodes using Antibody Conjugation Kit (Akoya Biosciences). Prior to CODEX imaging ...
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bioRxiv - Microbiology 2024Quote: ... was incubated at ambient temperature for 30 min and detected with HRP-conjugated goat anti-rat and visualized with Opal 620 (cat#OP-001004, Akoya, Marlborough, MA). The LANA signal was quenched using the Leica kit’s peroxide block ...