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Citations for Akoya Biosciences :
51 - 100 of 130 citations for Adenylyltransferase And Sulfurtransferase MOCS3 MOCS3 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... followed by incubation with the first primary antibody for 1 hour, subsequently with the secondary antibody (Polymer HRP, Ms + Rb (Akoya biosciences, MA)) for 30 minutes and finally with an Opal fluorophore ((Akoya biosciences ...
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bioRxiv - Immunology 2022Quote: Commercially available oligonucleotide conjugated antibodies were purchased from Akoya Biosciences and used at a standard dilution of 1/200 (Supplemental Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: Commercially available oligo-conjugated antibodies were obtained from Akoya Biosciences (Marlborough ...
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bioRxiv - Cancer Biology 2021Quote: Tissue sections were baked for 3 hours at 62°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 minutes of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001).
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bioRxiv - Immunology 2024Quote: ... Biotinylated antibodies were visualized with Streptavadin-AF594 (Cat. No.: S32356) and the CD4 antibody was visualized with OPAL 520 (Akoya Biosciences, Cat. No.: FP1487001KT). The ROI’s selected to undergo GeoMx® DSP spatial transcriptomics were chosen based on Lyve-1 staining ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 2.5 hours at 63°C in vertical slide orientation with subsequent deparaffinization performed on the Leica Bond RX followed by 30 min of antigen retrieval with Leica Bond ER2 followed by 6 sequential cycles of staining with each round including a 30-minute combined block and primary antibody incubation (Akoya antibody diluent/block ARD1001EA) except HER2 ...
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bioRxiv - Physiology 2022Quote: ... antibodies were reduced using a “Reduction Master Mix” (Akoya Biosciences) to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences ...
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bioRxiv - Physiology 2022Quote: ... Antibodies were imaged cyclically using the CODEX system from Akoya Biosciences and a Keyence BZ-X810 slide scanning microscope fitted with a 20x air objective (0.75 NA) ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... followed by horseradish peroxidase (HRP)-conjugated secondary antibody polymer (Akoya), and HRP-reactive OPAL fluorescent reagents (Akoya ...
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bioRxiv - Immunology 2024Quote: ... slides were blocked with 1X antibody diluent/block (Akoya Biosciences) for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies were coupled to OPAL520 (AKOYA Biosciences Cat# FP1487001KT), OPAL570 (AKOYA Biosciences Cat#FP1488001KT) ...
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bioRxiv - Genomics 2023Quote: ... and incubated with the primary antibody before being probed with an appropriate OPAL™ secondary antibody conjugated to a fluorophore (Akoya Biosciences, Supplementary Table 21). This was followed by a further HIER to remove the primary antibody before staining with the next primary/secondary combination ...
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 1X Antibody Diluent/Block (Akoya Biosciences) for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Opal anti-mouse and rabbit HRP secondary antibody (Akoya Biosciences, #ARH3001KT) was used without dilution ...
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bioRxiv - Neuroscience 2024Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies were detected with OPAL Polymer HRP Ms + Rb (Akoya Biosciences) for 15 minutes and visualized following a 10-minute incubation with Tyramide signal amplification and Opal Fluorophore (Akoya ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were diluted in 1X Plus Automation Amplification Diluent (Akoya Biosciences). Primaries were detected using according anti-species secondary antibodies conjugated to HRP (OmniMap Ventana ...
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bioRxiv - Cancer Biology 2024Quote: ... Scanning of the full antibody panel was performed on PhenoImager (Akoya Biosciences). A spectral fluorophore for each primary antibody and autofluorescence library was made to enable optimal multispectral unmixing ...
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bioRxiv - Cancer Biology 2024Quote: ... rinsed in dH2O and blocked using 1x Antibody diluent/Block solution (Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase activity was blocked using Antibody Diluent/Block (Akoya Bioscience, US) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Secondary antibodies were obtained from Akoya (OPAL 690, OPAL 520, and OPAL 570) and diluted to a concentration of 1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... panCK staining was performed by using an OPAL TSA-DIG antibody (Akoya Biosciences) in combination with an OPAL 780 fluorophore conjugated anti-DIG antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with anti-rabbit HRP-conjugated secondary antibody (Akoya Biosciences, NEL840001KT). Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences ...
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bioRxiv - Pathology 2024Quote: ... To validate the efficacy of commercial DNA-barcode-conjugated antibodies for PhenoCycler by Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by incubation with secondary antibodies ImmPRESS-HRP anti mouse/rabbit (Akoya Biosciences, ARH1001EA) or ImmPRESS-anti rat HRP (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Signal for antibody complexes was labeled and visualized by their corresponding Opal Fluorophore Reagents (Akoya) by incubating the slides for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... The tissue section was stained with a cocktail of DNA-barcoded antibodies purchased from Akoya company (CD4/EPR6855 ...
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bioRxiv - Bioengineering 2023Quote: The antibody conjugation and the staining were done according to the manufacturer’s protocol (Akoya Biosciences). Details regarding primary antibody ...
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bioRxiv - Immunology 2021Quote: ... antibodies was performed using the OPAL Automation Multiplex IHC Detection Kit (Akoya Biosciences, Catalog 160 #NEL830001KT) implemented onto a BOND Research Detection System (DS9455) ...
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bioRxiv - Bioengineering 2022Quote: ... All sections were then incubated with an Opal 520 polymer anti-rabbit HRP antibody (Akoya Biosciences) and counterstained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a 10-minute incubation with Opal Antibody Dilutent/Block solution (Akoya Bioscience, Marlborough, USA). The first primary antibody was then applied overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies were detected by incubation for 10 min with HRP-Polymer (Akoya Biosciences, Marlborough, USA) and subsequent TBST washing for 3x 3 min ...
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bioRxiv - Immunology 2024Quote: ... One IF cycle consisted of 10 min protein blocking using antibody diluent (Akoya Biosciences, Marlborough, USA) followed by 3x 2 min washing with 1x TBST ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Microbiology 2024Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Pathology 2024Quote: ... Sections with the fibulin-1 antibody were incubated with Opal 650 reagent (1:200, FP1496001KT, Akoya Biosciences) diluted in 100mM borate buffer pH 8.5 containing 0.1% Tween-20 ...
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bioRxiv - Immunology 2021Quote: ... RNA probes and panCK antibody were detected with the following Opal Fluorophores pairings: CXCL13 Opal 480 (Akoya FP1500001KT), CXCL10/11 Opal 520 (Akoya FP1487001KT) ...
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bioRxiv - Immunology 2022Quote: ... Each target was linked using the OPAL Polymer HRP conjugated secondary antibodies (ready-to-use, ARH1001EA, AKOYA Biosciences). Visualization of the different targets was established using the OPAL Polaris 570 (FP1488001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed in TBS + 0.1% tween (TBS-T) and blocked in Antibody Diluent/Block (Akoya Biosciences, ARD1001EA) for 30 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... The binding of antibodies were detected with a 1:150 dilution of OPAL 520 Reagent (AKOYA, SKU FP1487001KT). Slides were digitally imaged using an Aperio ScanScope FL Scanner (Leica Biosystems).
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bioRxiv - Physiology 2024Quote: ... twice for five minutes each followed by incubation in blocking buffer (Opal Antibody Diluent/Block, Akoya Biosciences ARD1001EA) for ten minutes.
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bioRxiv - Pathology 2024Quote: Slides were incubated with primary antibodies (Table 2) diluted in 1X Plus Automation Amplification Diluent (FP1609, Akoya Biosciences) in the following sequential order ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...