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Citations for Akoya Biosciences :
51 - 100 of 186 citations for 5 NAPHTHALEN 1 YL 2H PYRAZOL 3 YLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... sections following HPR-C1 treatment were incubated with TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Cancer Biology 2023Quote: Seven color multiplex-stained slides were imaged using the Vectra Multispectral Imaging System version 3 (Akoya). Scanning was performed at 20x (200x final magnification) ...
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bioRxiv - Molecular Biology 2023Quote: ... the development reaction was carried out using the TSA Plus Cyanine 3 Kit (Akoya Biosciences, US) (FISH ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Immunology 2021Quote: ... slides were scanned using the Vectra® 3 automated quantitative pathology imaging system (Vectra 3.0.5; Akoya Biosciences). Multispectral images were unmixed and analysed using the inForm Advanced Image Analysis Software (inForm 2.4.6 ...
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bioRxiv - Microbiology 2023Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Developmental Biology 2024Quote: ... after which the second color was generated by Cyanine 3 incubation (NEL744001KT, Akoya biosciences, Marlborough, MA, USA) for five minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound probes were detected using TSA Plus fluorophores Cyanine 5 (Akoya Bioscience, NEL745001KT) and Cyanine 3 (Akoya Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were imaged at both 10x and 20x using the Vectra 3 Polaris and Vectra imaging software (Akoya Biosciences). Acquired qpTIFF images from the Vectra Polaris system were imported into QuPath analysis software(84 ...
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bioRxiv - Immunology 2021Quote: ... Multispectral images were acquired using a Vectra® 3 imaging system employing the Vectra3 and Phenochart software (all Akoya Biosciences). Images are displayed in pseudocolours ...
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
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bioRxiv - Neuroscience 2021Quote: ... the RNAscope probe for TNFR1 (product #408111) and the TSA Cyanine 3 Plus Evaluation kit (product #NEL744001KT, Akoya Biosciences, Boston, MA, USA). After completing the FISH protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Opal dyes were used at 1/750-1/2250 (Akoya Biosciences FP1487001KT ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed before ISH signal was developed with the TSA Plus Cyanine 5 System (Akoya Biosciences # NEL745001KT). Slides were then stripped of horseradish peroxidase and blocked with normal goat serum before incubating with goat anti-rabbit Alexa Fluor 488 (1:400 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 3 times in PBS and incubated in final fixative solution (1000uL of PBS + 20uL of Akoya’s final fixation reagent) for 20 minutes at room temperature.
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bioRxiv - Developmental Biology 2023Quote: ... Opal 620 (1:750) and Opal 690 (1:750) (Akoya Biosciences) diluted in TSA buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence signals were amplified and visualized using the TSA Plus Cyanine-5 and the TSA Plus Fluorescein systems (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... 5 ROIs in 34 WSI from the melanoma dataset were spectrally unmixed using inForm® software v6.4.2 (Akoya Biosciences), and analyzed with the HALO® image analysis platform (Indica Labs) ...
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bioRxiv - Immunology 2021Quote: ... dissolved 1:50 in 1 X Plus Amplification Diluent (Akoya biosciences, MA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 2hr with HRP-conjugated anti-mouse IgG diluted at 1:300 and followed by 1 hr incubation of TSA plus Cyanine-2 (1:300; cat#NEL745001KT, Akoya Biosciences). Sections were counterstained with DAPI and mounted on slides with CC/Mount mounting medium (cat# C9368 ...
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bioRxiv - Neuroscience 2021Quote: ... was applied for channel 1 and Opal™ 520 (Akoya; #FP1487001KT; 1:1500) was applied for channel 2 ...
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bioRxiv - Cell Biology 2023Quote: ... with respective dilutions at 1:750 and 1:1500 (Akoya Biosciences, Waltham, MA). We mounted slices with ProLong Gold Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI (1:5000, Akoya Biosciences) was spiked into FACS tubes directly prior to analysis on a Cytek Aurora in order to detect live/dead cells ...
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bioRxiv - Genomics 2021Quote: ... 323100) with the V-nCoV2019-S-C3 probe (ACD Bio, 848561-C3) and the TSA Plus Cyanine 5 fluorophore (Akoya Biosciences, NEL745001KT). For samples D13-17 ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer and incubated with Cy5-tyramide solution (TSA Plus Cyanine 5; AKOYA Biosciences, NEL745001KT) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution with secondary 620 reagent (1:100) (Akoya Biosciences) and peak wavelength at 588/616 ...
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bioRxiv - Cell Biology 2023Quote: ... at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Developmental Biology 2022Quote: ... and Opal dyes 520 (1:1000 dilution) and 620 (1:1500 dilution) (FP1487001KT, FP1495001KT, Akoya Biosciences). We performed the staining on 5-micrometer paraffin-embedded ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 520 (Akoya #FP1487001KT; 1:1500), OpalTM 570 (Akoya #FP1488001KT ...
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bioRxiv - Developmental Biology 2023Quote: ... OpalTM 570 (Akoya #FP1488001KT; 1:1500) and OpalTM 690 (Akyoya #FP1497001KT ...
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bioRxiv - Immunology 2022Quote: ... conjugated primary antibodies cocktail (Akoya CD3e 1:100 and Akoya CD20 1:200 in Abcam Antibody Diluent) 2x for 90 minutes each ...
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bioRxiv - Neuroscience 2021Quote: ... Opal™ 570 (Akoya; #FP1488001KT; 1:1500) was applied for channel 1 and Opal™ 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 690 dyes (1:1000, Akoya Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... and Opal 570 (1:1500, Akoya FP1488001KT), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:15,000 dilution followed by secondary antibody 570 opal reagent (1:100 dilution) (Akoya Biosciences) and peak wavelength at 555/570 ...
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bioRxiv - Cancer Biology 2021Quote: ... staining was performed at 1:100 dilution followed by secondary antibody 520 reagent (1:100) (Akoya Biosciences; Menlo Park, California) and peak wavelength at 494/525 ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 570 (1:500, Akoya FP1488001KT) reagent incubation for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP conjugated goat anti rabbit (1:100, Table S1) diluted 2% NHS for 1.5hr followed by OPAL 650 (1:500, Akoya FP1496001KT) reagent incubation for 15 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 1/2000 for TSA-CY3 (Akoya Biosciences, NEL744001KT) and 1/1000 for TSA-CY5 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... and 690 fluorophores (Akoya Biosciences, diluted 1:200) respectively ...
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bioRxiv - Immunology 2022Quote: ... Opal 570 reagent 1:100 (NEL810001KT, Akoya Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Amplification and HRP-C1 and HRP-C2 signal development were performed as recommended by the manufacturer with the modification of the use of 0.5× RNAScope wash buffer instead of 1× RNAScope wash buffer and use of a 1:150 dilution of Opals (all from Akoya Bioscience) instead of 1:1500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Reporter stock solution was prepared containing 1:300 Hoechst stain and 1:12 dilution of assay reagent in 1x CODEX buffer (Akoya Bioscience). Fluorescent oligonucleotides (Akoya Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... The hybridization detection was carried out using Opal Fluorophore Reagent Packs Opal-520 (channel (C) 1)/570 (C2)/690 (C3) (1:1500) (Akoya Biosciences). Probes (Advanced Cell Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: ... channel 1 was established and the slices were incubated in a 1:750 solution of Opal 570 (Akoya Biosciences, catalog # FP1488001KT) diluted in TSA Buffer (ACD) ...
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bioRxiv - Neuroscience 2023Quote: ... Opal dyes (1:1000) were assigned for each channel: Assay # 1: tdTomato expression was identified by Opal 690 (Akoya Bioscience, catalog # FP1497001KT), Vglut2 expression was identified by Opal 570 (Akoya Bioscience ...