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Citations for Eppendorf :
251 - 300 of 335 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 8μl of the eluted 12μl aRNA was transferred into a new 96-well PCR plate (Eppendorf, Germany).
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bioRxiv - Genomics 2020Quote: ... using a plate sealer (PX1 Plate Sealer #181-4000) for droplet PCR amplification (Eppendorf Mastercycler Pro #E90030010). The following cycling conditions were used ...
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bioRxiv - Bioengineering 2021Quote: ... 80% ACN into PCR tubes and dried at 45 °C in a SpeedVac centrifuge (Eppendorf, Concentrator plus). Peptides were resuspended in 0.1% TFA ...
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bioRxiv - Physiology 2021Quote: ... the droplets generated from each sample were transferred to a 96-well PCR plate (Eppendorf, Hamburg, Germany). PCR amplification was carried out on a T100 thermal cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... PCR amplifications were carried out with 31 cycles in a programmable thermal cycler (Eppendorf AG, Hamburg, Germany). The program used was ...
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bioRxiv - Genomics 2021Quote: ... snap-frozen in five aliquots (Safe-Lock cup DNA LoBind 2 ml PCR clean tubes, Eppendorf, #0030108078) and stored at -80 °C ...
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bioRxiv - Genomics 2020Quote: ... qRT-PCR was performed on 25ng total DNA per reaction in duplicates on Mastercycler ep realplex2 (Eppendorf) and with Absolute QPCR SYBR Green Mix (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed with an annealing temperature of 59 °C and 37 cycles using Mastercycler Pro (Eppendorf).
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bioRxiv - Neuroscience 2023Quote: ... Cells fell into the cap of a sterile collection tube (0.2 ml PCR tubes, Eppendorf AG, Germany), containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µL of PCR products from each plate were then pooled into DNA LoBind tubes (Eppendorf 022431021) for purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA was denatured for 5 min at 95 °C in a PCR thermocycler (Eppendorf Mastercycler), then incubated at 65 °C for 60 min ...
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bioRxiv - Neuroscience 2024Quote: ... PCR amplifications were carried out with 35 cycles in a programmable thermal cycler (Eppendorf AG, Hamburg, Germany). The program used was ...
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bioRxiv - Molecular Biology 2020Quote: ... The Chromosome-specific SSR marker PCR was performed in a DNA thermocycler (Mastercycler Nexus GSX 1 Eppendorf, Germany) using the following cycling parameters ...
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bioRxiv - Genomics 2020Quote: ... after which 70μl of the released material was collected in a new 96-well PCR plate (Eppendorf, Germany). When a Cy3 fluorescent cDNA activity print was needed for tissue optimization ...
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bioRxiv - Systems Biology 2021Quote: ... 19% ddH2O into PCR tubes and finally dried at 60 °C in a SpeedVac centrifuge (Eppendorf, Concentrator plus). Peptides were resuspended in 0.1 % TFA ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μL of the resulting droplet emulsion was transferred to a semi-skirted 96 well PCR plate (Eppendorf), sealed with foil and amplified on a thermal cycler using the following amplification conditions ...
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bioRxiv - Microbiology 2020Quote: The DNA sequence (308 bp) was amplified by PCR in a Mastercycler Pro 6321 (Eppendorf AG, Hamburg, Germany). The components of the PCR reaction mix (50 μl per reaction ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... Antibody binding was conducted in the volume of 50 µl on thermomixer for 96-well PCR plates (Eppendorf) in 4 °C cold room for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the following steps were performed: After having collected single cells (see 2.4) in single nuclease-free 0.2 mL PCR-tubes (Eppendorf AG ...
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bioRxiv - Microbiology 2020Quote: ... the polymerase chain reaction (PCR) was performed on a Mastercycler ep Gradient S thermal cycler (Eppendorf, Hamburg, Germany) using a slow ramp in temperature (0.1 °C s−1 ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were conducted using the following thermocycling parameters in a Mastercycler Pro 6321 (Eppendorf AG, Hamburg, Germany): 94 °C for 3 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
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bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s instructions and the emulsions (approximately 40 µl) were transferred to a 96-well PCR plate (twin-tec, Eppendorf) and sealed with foil (Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: 1000 cells of CD4+ cells were sorted directly into 5 ul of TCL buffer (Qiagene, Cat#1031576) in skirted Eppendorf 96-well twin.tec PCR plate (Eppendorf). Smart-seq2 modified from a published method [13] was carried out at Broad Technology Labs ...
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bioRxiv - Genomics 2019Quote: ... One µl of this cocktail was added to the PCR mixture and placed in a thermocycler (Eppendorf MasterCycler Pro). Thermocycling settings were as follows ...
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bioRxiv - Immunology 2020Quote: ... and RNAse free water for a final volume of 13 µl in 0.2 ml PCR Tube Strips (732-0098, Eppendorf). The reaction tubes were transferred into a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... PCR amplifications were carried out on an Eppendorf® Mastercycler® Nexus X2 Thermal Cycler (Eppendorf Co., Ltd. Germany) using PrimeSTAR HS DNA polymerase (Takara Biotechnology Co. ...
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bioRxiv - Genomics 2021Quote: ... Positive droplets were gated and sorted out into 15 µL of PCR grade water in a 1.5 ml DNA LoBind tube (Eppendorf).
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription of GEMs was performed in a Eppendorf Mastercycler X50s thermal cycler using 0.2 ml PCR tube strips (E0030124286, Eppendorf): 53 °C for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... using 50 ng of Geneblock as template and 0.5 μM of forward and reverse primer in a PCR Mastercycler (Eppendorf), with initial heating at 98°C for 30s ...
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bioRxiv - Developmental Biology 2023Quote: ... all isolated fresh blastomeres or cells were washed three times with CUT&RUN wash buffer to avoid possible contamination and then transferred into a 1.5mL low-binding PCR tube (Eppendorf) containing 100µL of the wash buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... The bones of the hind limbs were cut transversally in the middle of the diaphysis and the bone marrow was harvested by centrifuging for 30 sec on 5500 g (after placing the bones with the opened sides down in PCR tubes placed in 1.5 ml Eppendorf tubes). The bone marrow was resuspended in culture medium ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2020Quote: ... 190 μl of the lysate was combined with 10 μl of 20 mg/ml proteinase K in a PCR plate and incubated in a MasterCycler ProS (Eppendorf) for 60 min at 60°C for digestion ...
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2022Quote: ... Cells from each brain region were sorted in a separate A1 well of a 96 well PCR plate (Eppendorf, 951020401) containing 100 µl of PBS buffer with 0.3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 1mM PMSF(sigma), orange G dye (adopted form (von der Haar, 2007)) and moved to 384 wells PCR plate (Eppendorf) with reusable lid (4tetude) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 96-well plate was sealed with a sterile and optically transparent sealing film (PCR-SP-S, AxySeal Scientific) and covered with a polystyrene plate lid (Eppendorf) that had silicone grease lining the edges ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the tip of the pipette was broken into a single nuclease-free 0.2 mL PCR-tube (Eppendorf AG, Hamburg, Germany), pre-filled with 9 µL Single Cell Lysis Solution and 1 µL Single Cell DNAse ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Bound phosphopeptides were eluted 2 times with 30 μl elution buffer (40 % ACN, 15 % NH4OH (25 %, HPLC grade)) each and collected by centrifugation into clean PCR tubes (Eppendorf). Samples were concentrated in a SpeedVac for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
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bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...
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bioRxiv - Cancer Biology 2023Quote: Single hemocytes were sorted directly into a 384-well plate containing 2.3 µl of lysis buffer (Eppendorf twin.tecTM PCR plates) using a CyCloneTM robotic arm and at highly stringent single cell sort settings (single mode ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Genomics 2023Quote: ... gating was established and single cells were sorted (130 micron nozzle assembly) into low-bind 96 well PCR plates (Eppendorf twin.tec LoBind ...
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bioRxiv - Genomics 2024Quote: ... 3uL of SL-B reagent (BioSkryb Genomics, USA) was deposited in each well of a LoBind twin.tec PCR plate (Eppendorf, Germany) prior to sorting ...
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bioRxiv - Genetics 2019Quote: ... 30μl of PBS + 2.5% FBS was added and mixed into each well with a multi-channel pipette and 25 μl (half) was transferred to a 96 well PCR plate (Eppendorf, 951020362) for subsequent genomic DNA extraction (the PCR plate was sealed and stored at −80C) ...
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bioRxiv - Systems Biology 2021Quote: ... The six 96-well master plates were then re-arrayed into the final 384 well v-bottom PCR Plates (Eppendorf #951020702).