Labshake search
Citations for Eppendorf :
251 - 294 of 294 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells from each brain region were sorted in a separate A1 well of a 96 well PCR plate (Eppendorf, 951020401) containing 100 µl of PBS buffer with 0.3% BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... 1mM PMSF(sigma), orange G dye (adopted form (von der Haar, 2007)) and moved to 384 wells PCR plate (Eppendorf) with reusable lid (4tetude) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... the 96-well plate was sealed with a sterile and optically transparent sealing film (PCR-SP-S, AxySeal Scientific) and covered with a polystyrene plate lid (Eppendorf) that had silicone grease lining the edges ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... the tip of the pipette was broken into a single nuclease-free 0.2 mL PCR-tube (Eppendorf AG, Hamburg, Germany), pre-filled with 9 µL Single Cell Lysis Solution and 1 µL Single Cell DNAse ...
-
bioRxiv - Microbiology 2021Quote: ... was performed with 0.5 µg of ligated DNA with oligonucleotides 6212 and 6420 in a standard PCR program with a 3 min extension time on a Mastercycler thermocycler (Eppendorf). Phusion DNA polymerase (NEB ...
-
bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
-
bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
-
bioRxiv - Microbiology 2020Quote: ... qPCR was performed with the realplex2 Mastercycler epgradient S automated real-time PCR system (Eppendorf®, New York City, NY) with standard reaction conditions (Table S8) ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was carried out using Maxima SYBR Green qPCR Master Mix (2X) (Molecular Biology) and Eppendorf Realplex System (Eppendorf) following the manufacturers’ protocol ...
-
Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Bound phosphopeptides were eluted 2 times with 30 μl elution buffer (40 % ACN, 15 % NH4OH (25 %, HPLC grade)) each and collected by centrifugation into clean PCR tubes (Eppendorf). Samples were concentrated in a SpeedVac for 15 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
-
bioRxiv - Genomics 2023Quote: ... gating was established and single cells were sorted (130 micron nozzle assembly) into low-bind 96 well PCR plates (Eppendorf twin.tec LoBind ...
-
bioRxiv - Cancer Biology 2023Quote: Single hemocytes were sorted directly into a 384-well plate containing 2.3 µl of lysis buffer (Eppendorf twin.tecTM PCR plates) using a CyCloneTM robotic arm and at highly stringent single cell sort settings (single mode ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were transferred to a PCR-strip tube and heated for 3 min using a Mastercycler Nexus X2 thermal cycler (Eppendorf). After heating ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...
-
bioRxiv - Genomics 2024Quote: ... 3uL of SL-B reagent (BioSkryb Genomics, USA) was deposited in each well of a LoBind twin.tec PCR plate (Eppendorf, Germany) prior to sorting ...
-
bioRxiv - Genetics 2019Quote: ... 30μl of PBS + 2.5% FBS was added and mixed into each well with a multi-channel pipette and 25 μl (half) was transferred to a 96 well PCR plate (Eppendorf, 951020362) for subsequent genomic DNA extraction (the PCR plate was sealed and stored at −80C) ...
-
bioRxiv - Systems Biology 2021Quote: ... The six 96-well master plates were then re-arrayed into the final 384 well v-bottom PCR Plates (Eppendorf #951020702).
-
bioRxiv - Systems Biology 2020Quote: ... 5 Ml/well of the product from each 1st PCR plate was pooled into one specific well of the collection plate (Deepwell plate 96/500 μ!, Eppendorf; each well containing all 96 samples from one 1st PCR plate) ...
-
bioRxiv - Microbiology 2019Quote: ... similar reaction mixture of PCR except for 1×SYBR Green Real-time PCR Master Mix (Toyobo, Tokyo, Japan) was used and performed at Mastercycler EP realplex2 (Eppendorf, Germany). To estimate the copy numbers of aCPSF1 or RNAP captured genes ...
-
bioRxiv - Microbiology 2021Quote: 96 μl of the final OIL-PCR master mix was aliquoted into a 500 μl deep well plate (Eppendorf 00.0 501.101). Nycodenz purified stool cells were diluted in PBS to 104 cells/μl in an 8-well PCR strip for multichannel pipetting ...
-
bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was run with 2 μl of cDNA and qPCRBIO SyGreen master mix (Cat.# PB20.11-01, PCR Biosystems) on an Eppendorf Mastercycler Ep Realplex (Eppendorf, Hauppauge, NY) using a program of 15 min at 95°C ...
-
bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
-
bioRxiv - Genomics 2022Quote: ... 70μl of the released cDNA material from each hybridization chamber well were collected and stored in a 96-well PCR plate (Eppendorf, Germany).
-
bioRxiv - Genomics 2022Quote: ... 70μl of the released material from each well were collected and stored in a new 96-well PCR plate (Eppendorf, Germany). Library preparation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was done with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne, Tartu, Estonia) on RealPlex4 thermocycler (Eppendorf, Hamburg, Germany). For miRNA detection ...
-
bioRxiv - Genomics 2023Quote: ... The nuclei were diluted to ∼300 nuclei per uL across 2 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512). The reverse transcription reaction was set up as in Cao et al. ...
-
bioRxiv - Microbiology 2023Quote: ... This PCR master mix was split into triplicates (16.7 μL each reaction) using an epMotion 5075 liquid handling robot (Eppendorf, Hamburg, Germany). Thermocycler conditions were 2 min at 95 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
-
bioRxiv - Microbiology 2021Quote: ... 10 µL was transferred to a PCR-clean polypropylene V bottom 96-well plate and covered with a silicone seal (Eppendorf, Mississauga, ON). Reactions were incubated at 29°C for 16 hours and final reactions stored at -20°C ...
-
bioRxiv - Immunology 2021Quote: ... cell populations were distinguished by WGA and DRAQ5 signals and with individual cells sorted into each well of a 384-well plate (twintec™ PCR plates, Eppendorf, Germany) containing 2.3 µl lysis buffer (Picelli et al. ...
-
bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
-
bioRxiv - Immunology 2021Quote: ... Resulting cDNA was diluted four-fold and subjected to end-point PCR using Taq DNA Polymerase (GeneDirex Inc., Taoyuan, Taiwan) in a Mastercycler Nexus (Eppendorf, Hamburg, Germany) thermocycler ...
-
bioRxiv - Genomics 2020Quote: ... We included an overnight digestion step in which tissues were lysed in a 96-well PCR plate at a constant temperature of 55° C on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hamburg, Germany), and we included 10 uL Proteinase K solution ...
-
bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
-
bioRxiv - Genomics 2022Quote: ... The remaining PIP emulsion was subjected to enzymatic lysis at 65°C for 35 min on a PCR thermocycler (Eppendorf Mastercycler Pro) with lid temperature set to 105°C ...
-
bioRxiv - Immunology 2020Quote: ... cells were sorted from cryopreserved PBMC and T cell lines as positive controls using CD1b tetramers loaded with GMM or purified SGL into single wells of a 96-well PCR plate (Eppendorf, Hamburg, Germany), which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen ...
-
bioRxiv - Cell Biology 2022Quote: The beads were resuspended with 40 µL extension mix and incubated at 37°C on a PCR cycler (Eppendorf, UX-93944-39) for 10 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... All reactions were run using the following touchdown PCR programme using an Eppendorf vapo.protect Mastercycler pro 384 (Eppendorf AG, 22331, Hamburg, Germany): 94°C for 15 minutes ...
-
bioRxiv - Immunology 2020Quote: ... The complementary DNA was catalyzed by incubating the samples at 25°C (10min) and 50°C (50min) in a PCR thermal cycler (Mastercycler X59s, Eppendorf, Hauppauge, NY, USA). The reaction was inactivated at 70°C for 15 min and allowed to cool at 4°C for 10min ...
-
bioRxiv - Plant Biology 2023Quote: ... RT qPCR was performed using 2×Hieff qPCR SYBR Green Master Mix (Yeasen, Shanghai, China) and PCR reactions and fluorescence detection were performed in a Mastercycler ep realplex (Eppendorf, New York, NY). ACTIN 2 (ACT2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed using EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech, Daejeon, Korea) with a master cycler gradient (Eppendorf, Hamburg, Germany). PCR conditions were as follows ...
-
bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...