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Citations for Eppendorf :
51 - 100 of 294 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... with random hexamer primers running on a MasterCycler EpGradient 5341 thermal cycler (Eppendorf AG, Hamburg, Germany). Real-Time qPCR was performed on a StepOnePlus Real-Time PCR System using MicroAmp Fast 0.1 mL 96-well Reaction Plates (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... Nextera PCR program cycle was used for PCR amplification using Eppendorf Thermal Mastercycler (Eppendorf AG, Hamburg). The holding temperature was 10°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification was performed on Mastercycler® nexus SX1 PCR thermal cycler (Eppendorf AG, Hamburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1.5-mL PCR clean (Eppendorf # 022431021) or 96 well plates were used to process the samples.
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bioRxiv - Cancer Biology 2022Quote: ... in 0.2 mL PCR tubes (Eppendorf). Tissue sections were subjected to whole genome amplification using the Ampli1 WGA Kit (Silicon Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed in a Mastercycler® ep realplex real-time PCR system (Eppendorf, Germany). cDNA was amplified using a SYBR Green kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf) for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Mastercycler PCR system (Eppendorf, Belgium). Each reaction was performed in a 10 μL total reaction mixture using 1 μL of the DNA sample and 0.5 μM final primer concentration (EntF*-PCR primers ...
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bioRxiv - Cell Biology 2021Quote: ... in a Realplex 4 PCR machine (Eppendorf), and values were normalized to rpl-32 as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... in a 96-well PCR plate (Eppendorf) for a total volume of 0.1 ml ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed with an Eppendorf Mastercycler Ep Realplex instrument (Eppendorf AG, Hamburg, Germany) and the Kapa SYBR Fast qPCR kit (Kapa Biosystems ...
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bioRxiv - Bioengineering 2019Quote: ... The suspension was centrifuged at 500 rcf for 10 min RT (Eppendorf 5430; Rotor: F-35-6-30). These steps were repeated until a white cell pellet was obtained (indicating erythrocyte depletion) ...
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bioRxiv - Genomics 2021Quote: ... The suspensions were centrifuged for 5 minutes at 2,000g at RT using a tabletop centrifuge (Centrifuge 5810R, (Eppendorf)) ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf). The RT-qPCR data were analyzed with DataAssist (ABI ...
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bioRxiv - Immunology 2020Quote: ... and 200 nM forward and reverse primers (Supplemental Table 10) (Integrated DNA Technologies) on a Mastercycler realplex2 thermocycler (Eppendorf). Primers were designed using the qPCR setting on Primer3Plus (39) ...
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bioRxiv - Molecular Biology 2019Quote: Digital PCR sample on the glass substrate was amplified in a flat-panel PCR machine (6335CH703993, Eppendorf AG 22331 Hamburg). 2-3 drops of mineral oil were placed onto the heating block to help with the heat transfer ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 μL 0.1% TFA was added and the proteoCHIP LF 48 was cooled on wet ice to freeze the hexadecane again and allowed for manual separation from the sample containing aqueous phase by transferring the sample to individual wells of a low binding 96 well PCR plate (EP0030129512, twin.tec. PCR Plate 96 LoBind, skirted, Eppendorf, Darmstadt). The EVO96 proteoCHIP did not contain hexadecane and was directly placed on top of a 96well PCR plate to transfer the sample by centrifugation (500 g ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed in a FlexID Mastercycler (Eppendorf) using Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed in a thermocycler (Eppendorf, Germany) under the following thermal cycling conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... 25μl was transferred to a PCR plate (Eppendorf) and stored at −80°C for subsequent genotyping ...
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bioRxiv - Neuroscience 2020Quote: ... A Mastercycler 5333 PCR thermal cycler (Eppendorf, Germany) was used to perform reverse transcription and pre-amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR data was produced by Eppendorf Realplex2 master cycler (Eppendorf AG 223341 ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative PCR was performed on Mastercycler realplex4 (Eppendorf) using KOD SYBR® qPCR Mix (TOYOBO ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using Mastercycler® pro (Eppendorf) using the following cycling program ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR machine (Eppendorf AG 22331, Hamburg, Germany) was programmed as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... and re-dissolved in lysis buffer supplemented with 1% v/v SDS at RT in a Thermomixer R (Eppendorf) at 1200 rpm for 1-2 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: GV oocytes were microinjected with ~5 pl of cRNAs in M2 medium (with 2.5 mM milrinone and 3mg/mL BSA) at room temperature (RT) with a micromanipulator TransferMan NK 2 (Eppendorf) and picoinjector (Medical Systems Corp.) ...
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bioRxiv - Neuroscience 2023Quote: ... To make 10 μl of 20 μM primer pairs per each gene in V-shaped 96 well plate (Eppendorf, #951020401), 4 μl of forward and reverse primers were added to 2 μl H2O.
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR experiment was performed in 96-well PCR plate using Eppendorf Mastercycler® RealPlex with SYBR Green PCR Master Mix (Eppendorf, USA) as per the universal SYBR Green qPCR protocol where fluorescent product is detected during the last step of each cycle ...
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bioRxiv - Biophysics 2021Quote: The gp15-gp16 core complex was obtained in vitro after incubation of the purified proteins for 30 min at room temperature (RT) in a thermo block with shaking (Eppendorf). The proteins were incubated using 180 pmol each at a 1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Immunology 2022Quote: ... muris burden in infected mice was quantified using spleens via RT-qPCR using a Mastercycler ep Realplex (Eppendorf, Hamburg, Germany) (32 ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR was performed in a Thermocycler (Eppendorf mastercycler) with an initial denaturation step at 95°C for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... in a Mastercycler® ep realplex PCR device (Eppendorf). The annealing temperature was 60 °C and the elongation time at 72 °C was 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... PCR program in a Mastercycler Personal-system (Eppendorf, USA) was 94°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification was performed using a Mastercycler gradient (Eppendorf) with 95 °C for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf): 42°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and used for PCR in a thermocycler (BioRad, Eppendorf). Finally ...
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bioRxiv - Immunology 2022Quote: ... which were sealed with PCR microplate sealing foil (Eppendorf), and flash frozen prior to storage at −80°C.
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was performed on a RotorGene Q (Eppendorf) using SensiFAST SYBR Hi-ROX (Bioline) ...
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bioRxiv - Plant Biology 2023Quote: ... PCRs were performed using the Master Cycler realPlex2 (Eppendorf) in a 96-well reaction plate according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2023Quote: ... The primers were annealed at 95°C for 4 min followed by 70°C for 3 min in a thermocycler (Eppendorf, Hamburg, Germany). The reaction was allowed to cool slowly to room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... on a Mastercycler real-time PCR machine (Eppendorf, Hamburg, Germany). Results were normalized to three validated internal controls (actin ...
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bioRxiv - Microbiology 2020Quote: ... the plate was covered with translucent PCR film (Eppendorf AG) and placed into the ImageXpress Micro XLS microscope into an environmental chamber with 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The isolation was performed using 96-well PCR plates (Eppendorf) and a magnetic stand (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... 25μl of cells were transferred to a PCR plate (Eppendorf) and stored at −80°C for subsequent genotyping using the same protocol as described for single cell in vitro assay.
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bioRxiv - Molecular Biology 2022Quote: ... The sRNA libraries were then amplified with PCR (Eppendorf, Germany), purified ...
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bioRxiv - Microbiology 2021Quote: ... Fifty microliter PCR-clean filter tips (Eppendorf, Cat. #0030 014.430) were used in combination with an 8-channel 50-µL volume adaptor (Eppendorf) ...
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bioRxiv - Systems Biology 2021Quote: ... into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of our custom lysis buffer (80 mM TEAB pH 8.5 ...