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Citations for Eppendorf :
301 - 335 of 335 citations for hsa mir 146a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... 5 Ml/well of the product from each 1st PCR plate was pooled into one specific well of the collection plate (Deepwell plate 96/500 μ!, Eppendorf; each well containing all 96 samples from one 1st PCR plate) ...
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bioRxiv - Microbiology 2020Quote: ... we used the universal bacterial primers 27F and 1492F with the following PCR conditions on a Mastercycler pro (Eppendorf, Hamburg, Germany); 95 °C for 5 min for DNA denaturation ...
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bioRxiv - Microbiology 2021Quote: 96 μl of the final OIL-PCR master mix was aliquoted into a 500 μl deep well plate (Eppendorf 00.0 501.101). Nycodenz purified stool cells were diluted in PBS to 104 cells/μl in an 8-well PCR strip for multichannel pipetting ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was run with 2 μl of cDNA and qPCRBIO SyGreen master mix (Cat.# PB20.11-01, PCR Biosystems) on an Eppendorf Mastercycler Ep Realplex (Eppendorf, Hauppauge, NY) using a program of 15 min at 95°C ...
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bioRxiv - Plant Biology 2019Quote: All primers were used for PCR amplification of genomic DNA using a touchdown program on the Mastercycler nexus GSX1 (Eppendorf, Germany): 95°C for 5 min ...
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bioRxiv - Genomics 2022Quote: ... the PCR products for apoLp and green fluorescent protein (GFP) were amplified from cDNA in a Mastercycler Nexus instrument (Eppendorf, Hamburg) with a 2-min initial denaturation at 94 °C followed by 30 cycles of 15 sec at 94 °C ...
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bioRxiv - Genomics 2022Quote: ... 70μl of the released cDNA material from each hybridization chamber well were collected and stored in a 96-well PCR plate (Eppendorf, Germany).
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bioRxiv - Genomics 2022Quote: ... 70μl of the released material from each well were collected and stored in a new 96-well PCR plate (Eppendorf, Germany). Library preparation ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was done with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne, Tartu, Estonia) on RealPlex4 thermocycler (Eppendorf, Hamburg, Germany). For miRNA detection ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were diluted to ∼300 nuclei per uL across 2 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512). The reverse transcription reaction was set up as in Cao et al. ...
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bioRxiv - Microbiology 2023Quote: ... This PCR master mix was split into triplicates (16.7 μL each reaction) using an epMotion 5075 liquid handling robot (Eppendorf, Hamburg, Germany). Thermocycler conditions were 2 min at 95 °C ...
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bioRxiv - Neuroscience 2023Quote: ... They were then resuspended to a concentration of ∼800-3000 nuclei per uL across 3-4 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512) in NSB ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL was transferred to a PCR-clean polypropylene V bottom 96-well plate and covered with a silicone seal (Eppendorf, Mississauga, ON). Reactions were incubated at 29°C for 16 hours and final reactions stored at -20°C ...
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bioRxiv - Immunology 2021Quote: ... cell populations were distinguished by WGA and DRAQ5 signals and with individual cells sorted into each well of a 384-well plate (twintec™ PCR plates, Eppendorf, Germany) containing 2.3 µl lysis buffer (Picelli et al. ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Immunology 2021Quote: ... Resulting cDNA was diluted four-fold and subjected to end-point PCR using Taq DNA Polymerase (GeneDirex Inc., Taoyuan, Taiwan) in a Mastercycler Nexus (Eppendorf, Hamburg, Germany) thermocycler ...
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bioRxiv - Genomics 2020Quote: ... We included an overnight digestion step in which tissues were lysed in a 96-well PCR plate at a constant temperature of 55° C on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hamburg, Germany), and we included 10 uL Proteinase K solution ...
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bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
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bioRxiv - Genomics 2022Quote: ... The remaining PIP emulsion was subjected to enzymatic lysis at 65°C for 35 min on a PCR thermocycler (Eppendorf Mastercycler Pro) with lid temperature set to 105°C ...
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bioRxiv - Immunology 2020Quote: ... cells were sorted from cryopreserved PBMC and T cell lines as positive controls using CD1b tetramers loaded with GMM or purified SGL into single wells of a 96-well PCR plate (Eppendorf, Hamburg, Germany), which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: The beads were resuspended with 40 µL extension mix and incubated at 37°C on a PCR cycler (Eppendorf, UX-93944-39) for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... All reactions were run using the following touchdown PCR programme using an Eppendorf vapo.protect Mastercycler pro 384 (Eppendorf AG, 22331, Hamburg, Germany): 94°C for 15 minutes ...
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bioRxiv - Immunology 2020Quote: ... The complementary DNA was catalyzed by incubating the samples at 25°C (10min) and 50°C (50min) in a PCR thermal cycler (Mastercycler X59s, Eppendorf, Hauppauge, NY, USA). The reaction was inactivated at 70°C for 15 min and allowed to cool at 4°C for 10min ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed using EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech, Daejeon, Korea) with a master cycler gradient (Eppendorf, Hamburg, Germany). PCR conditions were as follows ...
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bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
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bioRxiv - Neuroscience 2020Quote: ... Protein amounts were assessed using a Pierce BCA protein assay kit and spectrophotometer (Eppendorf BioPhotometer). Equal amounts of protein (15 μg ...
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bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate-based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmids used in initial identification screen for cis-regulatory elements used miniprep scale DNA purification (Zymo Research, D4020 or 5 prime/Eppendorf, FastPlasmid kit) and all subsequent experiments used midiprep scale DNA purification (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... we favoured magnetic beads to perform DNA extraction and lysates were processed for DNA extraction using the Macherey-Nagel™ NucleoMag® Tissue kit on an epMotion® 5075vt (Eppendorf, Hamburg – Germany). Volumes on the first binding step were adjusted to the starting volume of lysis buffer accordingly ...