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Citations for Eppendorf :
51 - 95 of 95 citations for Zika Virus Lysate PRVABC59 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... were made from single colonies of putative Xcm strains and cell suspensions (optical density (OD) = 0.3) were generated in MilliQ ultrapure H2O using a spectrophotometer (Eppendorf BioSpectrometer basic ...
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bioRxiv - Bioengineering 2023Quote: ... cerevisiae strain to 5 mL of YPD media and incubating in an orbital shaker incubator (Innova 42, Eppendorf, UK) at 30 °C and 250 rpm overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Lysate was cleared by centrifugation at 16.000 xg for 20 minutes at 4 °C (Eppendorf). Supernatant was collected and protein content was quantified by Bradford Spectrophotometry (Biorad) ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue lysates were centrifuged at 15,000 rpm at 4°C for 10 minutes (Eppendorf, 5424R). The supernatant was transferred to a new tube and protein concentration was measured with a DC Protein Assay Kit (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... Cell lysates were centrifuged (16,800g, 4°C) in a table-top centrifuge (Eppendorf, Hamburg, Germany) and stored at −80°C until use ...
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bioRxiv - Biochemistry 2021Quote: ... Unbound lysate was removed and the Dynabeads transferred to 2 ml LoBind protein tubes (Eppendorf). Beads were washed five times with 2% SDS wash buffer (150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lysates were cleared by a 30 min centrifugation at 3000 rpm (Eppendorf Centrifuge 5810 R) at 21 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cells in overnight cultures of Vibrio or Aliivibrio strains harboring transcriptional or translational fluorescent reporter plasmids were pelleted by centrifugation at 21,100 x g (Eppendorf 5424) and washed in AB medium ...
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bioRxiv - Molecular Biology 2022Quote: ... elongatus wild-type and mutant strains were inoculated into 800 μl of fresh BG11 medium supplemented with 0-35 ng/ml ciprofloxacin and spectinomycin (in the case of the mutant strains) in 48-well plates (Eppendorf). The plates were incubated at 300 rpm at 30°C under continuous light conditions and cell density was monitored by measuring OD750 every 12 hours ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Aliquots of 1.5 OD600 were made for each strain and pre-incubated at their restrictive temperatures for 10 min at 800 rpm in a Thermoblock (Eppendorf). Proteins were labelled for 5 mins (for soluble proteins ...
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bioRxiv - Microbiology 2023Quote: Overnight cultures of the investigated strains were prepared in 1 mL aliquots of TSB in 2 mL microcentrifuge tubes (Eppendorf) which were incubated horizontally with shaking (120 rpm ...
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bioRxiv - Biochemistry 2022Quote: ... Unbound lysate was aspirated and the beads transferred to a 2 ml LoBind protein tube (Eppendorf) using 2% SDS wash buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Genomics 2022Quote: ... 200 µl of cleared lysate was transferred to a new 2 ml tube (Eppendorf 0030 120.094) and used for the robot-assisted protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate underwent 1 hour of incubation at 56°C in a thermomixer (Eppendorf, Hamburg, Germany) with shaking ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates were centrifuged at 14,000 g for 10 minutes on a 5425R benchtop centrifuge (Eppendorf) to clarify the samples ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted lysates in 60% acetonitrile/0.1% formic acid were dried by vacuum centrifugation (Eppendorf; Concentrator Plus) at 45°C.
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bioRxiv - Systems Biology 2022Quote: ... Lysates were transferred into a reaction tube (Eppendorf low binding tube, 2 mL, Eppendorf, Hamburg, Germany), followed by addition of 500 µL ice-cold chloroform (CHCl3 ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Microbiology 2020Quote: Up-scale cultivation of the PmamDC45-trans-at-pks strain was done in a 10 L BioFlow® 120 Fermenter (Eppendorf) (34 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, Eppendorf #A-4-81 rotor) to remove intact cells and debris ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were centrifuged at 4 °C for 30 min at max speed (Eppendorf 5910R, 20133 rcf). The supernatants ...
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bioRxiv - Immunology 2021Quote: ... The obtained lysates were centrifuged for 20 min at 21,000 g and 4°C (Centrifuge 5424 R, Eppendorf) to precipitate insoluble fractions ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at 4°C at 3220 g for 15 min (Eppendorf #A-4-81 rotor) to remove intact cells and debris ...
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bioRxiv - Biochemistry 2021Quote: ... Production of CjLPMO10AFL was achieved by fed-batch fermentation of the expression strain in a 1-liter fermenter (DASGIP® benchtop bioreactors for cell culture; Eppendorf, Hamburg, Germany), essentially as described previously (44) ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then incubated at 37°C for 10 minutes at 1150 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000g for 2 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lysates were transferred to a new tube and were centrifuged for 2 min at full speed (Eppendorf centrifuge) and the supernatant was carefully mixed with 1 volume of 70% HPLC-grade ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Two ml of phage lysate with 109 PFU/ml were centrifuged at 14,000 rpm (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) to remove intact cells and debris ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
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bioRxiv - Molecular Biology 2022Quote: ... the resulting cell lysates were passed through a 20 G needle 100 times or heated using a Thermomixer (Eppendorf) at 55°C for 10 minutes with 1000 rpm agitation prior to the conventional RNA purification procedure in accordance with the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Genomics 2022Quote: ... possibly remaining parts of the tissue sections were scraped off with the pipette tip and the lysates were collected in nine separate DNA LoBind tubes (Eppendorf). The wells were washed once with 40 μL of lysis buffer and the washing solution was pooled with the lysate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation at 20817 × g for 10 minutes to remove all matrix particles the resulting lysate was transferred to a fresh 2.0 mL Lo-Bind tube (Eppendorf, Germany). DNA was isolated from the lysate using two approaches ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell lysate was refined by centrifuging at 18514 g for 40 min (Fixed angle rotor, 5810-R Centrifuge, Eppendorf). The clarified lysate was applied to a 5 mL HisTrap™TALON™ crude cobalt column (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
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bioRxiv - Molecular Biology 2023Quote: ... we favoured magnetic beads to perform DNA extraction and lysates were processed for DNA extraction using the Macherey-Nagel™ NucleoMag® Tissue kit on an epMotion® 5075vt (Eppendorf, Hamburg – Germany). Volumes on the first binding step were adjusted to the starting volume of lysis buffer accordingly ...