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Citations for Eppendorf :
1 - 50 of 67 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and the indicated RNA oligonucleotide diluted in nuclease-free water or just nuclease-free water using an Eppendorf GELoader (Eppendorf, 0030001222). The micromanipulator (Eppendorf InjectMan NI 2 ...
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bioRxiv - Genomics 2021Quote: ... 5 μL nuclease free water) and incubated for 45 min at 37°C at 1000 rpm (Eppendorf Thermomixer) for fragmentation ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was eluted in 30 μL nuclease-free water and stored at −80°C in low-bind tubes (Eppendorf) until further analysis.
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... that contains all the reaction components in a pre-mixed formulation and nuclease-free water to the RNA for cDNA synthesis in a thermal cycler (Eppendorf) using lab standardized cDNA synthesis method ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... In a sterile nuclease-free tube (Eppendorf #022600001), 5μl of annealed crRNA:tracrRNA was then complexed with 5μl of 1μg/μl Cas9 protein at 25°C for 15 minutes in 50μl nuclease-free ultrapure water (Thermo #J71786XCR) ...
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bioRxiv - Molecular Biology 2021Quote: ... Fertilization and microinjections were carried out as described previously5 using 50 ng/µl gRNA and 150 ng/µl cas9 mRNA in nuclease free water and a FemtoJet®4i (Eppendorf) microinjector ...
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bioRxiv - Genomics 2021Quote: ... nuclease free water and 8 ng of cDNA were run in triplicate using an automated pipetting system (epMotion M5073, Eppendorf, Hamburg, Germany), with no-template negative controls on a 384-well plate in a thermo-cycler (QuantStudio 12K Flex System ...
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bioRxiv - Neuroscience 2023Quote: ... and stored tissue pieces in nuclease free 1.5 mL polypropylene microcentrifuge tubes (Eppendorf) at -80 °C until further processing.
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bioRxiv - Bioengineering 2024Quote: ... placed in individual nuclease-free 2 mL centrifuge tubes (DNA LoBind® Tubes, Eppendorf) and homogenized using a rotor-stator homogenizer (TissueMiser ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the following steps were performed: After having collected single cells (see 2.4) in single nuclease-free 0.2 mL PCR-tubes (Eppendorf AG ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the tip of the pipette was broken into a single nuclease-free 0.2 mL PCR-tube (Eppendorf AG, Hamburg, Germany), pre-filled with 9 µL Single Cell Lysis Solution and 1 µL Single Cell DNAse ...
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bioRxiv - Immunology 2020Quote: ... and RNAse free water for a final volume of 13 µl in 0.2 ml PCR Tube Strips (732-0098, Eppendorf). The reaction tubes were transferred into a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... 100μL of RNAse free water with RNAsin (40 U/mL) was added and samples incubated in a thermomixer (Eppendorf) at 300 rpm for at 55°C for 1h for decrosslinking ...
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bioRxiv - Cell Biology 2019Quote: ... 1 nl of morpholino or mRNA diluted in nuclease-free water at desired concentrations were microinjected into the yolk along with 1% phenol red dye using FemtoJet microinjector (Eppendorf). Microinjections for rescue experiments were performed as two separate sets with initial mRNA injections followed by morpholino injections in the same embryo ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was diluted with RNAse free water to a concentration of 500ng/20ul and denatured at 70°C for 15min in a Mastercycler (Eppendorf). Mastermix was prepared by mixing ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... in RNase-free LoBind Eppendorf tubes (Eppendorf # 022431021) and snap-frozen by placing on dry ice ...
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bioRxiv - Cancer Biology 2021Quote: ... in RNase-free LoBind Eppendorf tubes (Eppendorf # 022431021) and snap-frozen by placing on dry ice ...
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bioRxiv - Developmental Biology 2021Quote: ... and 13 µl of water were incubated in a thermocycler (cat#EP950040025, Eppendorf) at 85°C for 3 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... then washed with an excess of deionized water with the aid of centrifugation (Eppendorf 5804 R centrifuge ...
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bioRxiv - Physiology 2022Quote: Sperm were concentrated by pipetting 2 mL sperm water into a microcentrifuge tube (Eppendorf), followed by centrifugation at 1500 x g for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The numbers of infective free viruses in culture supernatants (Eppendorf table centrifuge ...
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bioRxiv - Microbiology 2021Quote: ... few colonies from each isolate were mixed with molecular biology-grade water (Eppendorf, Hamburg, Germany), the mixture were centrifuged at 15,000 × g for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... we collected 50 mL water samples that were first centrifuged at 7,000 rpm (Eppendorf Centrifuge 5430R) at room temperature for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... transfection mixes were prepared in sterile DNase-free Eppendorf tubes (Eppendorf #0030108035) consisting of 100 µl Opti-MEM (Gibco #11058021) ...
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bioRxiv - Microbiology 2020Quote: ... water were added and incubated a second time under shaking (300 rpm, Thermomixer Comfort Typ 5, Eppendorf) at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and the mica surface was washed with water and dried with a Concentrator Plus (Eppendorf, Hamburg, Germany). To measure sample surface topography ...
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bioRxiv - Genomics 2020Quote: ... The cell-free supernatant was carefully pipetted into 2 mL LoBind tubes (Eppendorf LoBind microcentrifuge tubes ...
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bioRxiv - Neuroscience 2020Quote: ... The dry pellets were finally resuspended in water and the DNA quantified using the Biospectrometer (Eppendorf, Hamburg, Germany).
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discharged and the pellet was re-suspended in molecular biology-grade water (Eppendorf, Hamburg, Germany) and subjected to boiling at 100°C in a water bath for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... the numbers of free viruses and viable cells in supernatant and pellet fractions (Eppendorf table centrifuge ...
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bioRxiv - Biochemistry 2022Quote: ... cells were directly harvested into a DNAse-/RNase-free 1.5 mL tube (0030108051, Eppendorf). Nuclei were then lysed in 0.5 mL of ChAC-lysis buffer and the nuclei pellet was resuspended in 666 µL crosslinking buffer ...
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bioRxiv - Pathology 2022Quote: ... Urine was collected in preservative-free collection tubes (0.2mL PCR Tube, Eppendorf, Hamburg, Germany) and stored at -20°C until analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The agarose was first melted and dissolved in deionized water with thermomixer (Thermomixer C model 5382; Eppendorf, Hamburg, Germany) at the temperature of 80 °C and then cooled to 40 °C ...
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bioRxiv - Genomics 2021Quote: ... Positive droplets were gated and sorted out into 15 µL of PCR grade water in a 1.5 ml DNA LoBind tube (Eppendorf).
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bioRxiv - Physiology 2020Quote: ... Bone remnants were transferred into a 1.5 ml RNAse-free reaction tube (Eppendorf AG, Hamburg) containing 1 ml of TRIZOL reagent (Life Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... The solution was desalted on hydrophilic-lipophilic balance (HLB) cartridges (1cc, 30 mg)(Waters) and eluted in LoBind tubes (Eppendorf) before being dried down by Speed Vac.
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bioRxiv - Biochemistry 2021Quote: ... 10 mg ml−1 lactic acid in water) and added to 5 mg of pre-washed TiO2 beads and incubated for 30 min on a Thermomixer (Eppendorf) at 1200 rpm to keep the beads in suspension ...
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bioRxiv - Neuroscience 2022Quote: ... 200 ul of double distilled water (to get the content sticking to pestle and the wall of the Eppendorf tube) was added in the same soup and centrifugation was done at 3000 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... were diluted in water (1:10) and injected into the periplasm of the embryos during cellularization using a FemtoJet express microinjector (Eppendorf). The embryos used in this experiment expressed Utr-Venus and Sqh-mCherry which allowed us to monitor cell shape and actomyosin dynamics ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 0.1% Tween 20 in water) and centrifuged for 5 min at 500 g in a swinging bucket rotor centrifuge (5920R, Eppendorf). Nuclei pellets were resuspended in 1X Tagmentation Buffer (10 mM Tris pH 7.4 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... After reconstitution in 100 μL of ice-cold water the suspension was transferred to a pre-chilled cuvette and transformed at 630 kV using an Eporator (Eppendorf). 900 μL of LB was added ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were homogenised with a P200 pipette and collected in DNase/RNase free tubes (Eppendorf, 30108051). 200uL of chloroform (Sigma ...
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bioRxiv - Genetics 2023Quote: ... The cell-free preparation used for testing was obtained by centrifuging the culture (Minispin-plus; Eppendorf) for 7 minutes at 6000 rps and filtration.
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bioRxiv - Neuroscience 2020Quote: ... 200 mM solution in water) was then added to the samples and incubated for 4 hours at 21°C in a ThermoMixer (Eppendorf AG). 4 µL of 1M IAA (iodoacetamide in water ...
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bioRxiv - Systems Biology 2024Quote: ... the pellet from SM0 culture was washed three times with water and used to inoculate deep-well 96-well plates (Eppendorf, 10052143) filled with each of the 91 cultivation media containing perturbed metal concentrations (see Supplementary Table M1 for detailed media composition ...
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bioRxiv - Biochemistry 2020Quote: ... The absorption maximum was analysed at 595 nm against a blank value (demineralized water) using an UV/Vis spectrophotometer (BioPhotometer plus, Eppendorf, Hamburg, Germany). The dissolving procedure and spectrophotometric measurements were each performed in triplicate and the protein solubility was calculated according to the formula described by Zielińska et al ...
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bioRxiv - Cell Biology 2021Quote: Lipids were extracted from 100 mg of ground germlings overnight at 4 °C with 4.5 mL of methanol (MeOH): water (H2O) (25:75 by v/v) at 750 rpm (revolution per minute) in a thermomixer (Eppendorf, Hamburg, Germany). Lipids from 100 µl of conditioned media were extracted by vortexing for 2 min with 4.5 mL of methanol (MeOH) ...
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bioRxiv - Immunology 2022Quote: The pigments were diluted to 100 µg/ml with water or culture medium and they transferred in UVette® 220-1600 nm (catalog number: 0030106.300, Eppendorf, Montesson, France). Then ...