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Citations for Eppendorf :
1 - 50 of 69 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... was added to each library and incubated for two hours at room temperature while shaking using a Thermomixer C (Eppendorf, Hamburg, Germany). M2B2 beads (US Biological ...
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bioRxiv - Neuroscience 2021Quote: ... Each cage contained two 2 mL feeding vials (Eppendorf) pierced with two 2 mm holes at the bottom to allow drinking of the sucrose solutions they contained ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Two unscented stimuli were also present (empty Eppendorf tube) to control for the potential learning of visual cues ...
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bioRxiv - Developmental Biology 2022Quote: ... and a set of two CellTram’s (CellTram Vario, FemtoJet, Eppendorf). Each respective microinjector are connected to a holding micropipette for immobilizing the zebrafish embryo ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifugation procedure for two minutes at 10,000 x g (Eppendorf 5415 D ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we transferred the cell suspension to two 500ml centrifuge bottles (Eppendorf), and centrifuged both bottles at 1500rpm for 15 minutes at 4°C with neither acceleration nor deceleration ...
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bioRxiv - Microbiology 2022Quote: ... The detached cells were transferred to two-ml Safe-lock tubes (Eppendorf 0030120094) and homogenized for three minutes using a Bullet blender® homogenizer (Next Advance #BBY24M ...
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bioRxiv - Microbiology 2022Quote: ... and “Clean Up Libraries” in the protocol (Epmotion - Library Clean Up protocol, Eppendorf, Hamburg, Germany). On completion ...
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bioRxiv - Genomics 2021Quote: ... The strained sample was transferred into two LoBind 1.5 mL Eppendorf tubes (Cat # 022431021, Eppendorf) on ice ...
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bioRxiv - Microbiology 2019Quote: ... and centrifugation at 145000 RPM for two minutes at room temperature using MiniSpin tabletop centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... A two-step PCR-reaction was performed on a Mastercycler Pro instrument (Eppendorf, Wesseling-Berzdorf, Germany) with the following settings ...
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bioRxiv - Microbiology 2021Quote: ... Sedimentation of the sewage was commenced immediately using 50 ml Falcon tubes and two centrifuges (Eppendorf 5810R ...
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bioRxiv - Microbiology 2021Quote: ... 1xPBS) on 96 well plates for two hours and incubated on a MixMate thermomixer (Eppendorf, USA) at 25 °C under constant shaking (300 rpm) ...
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bioRxiv - Neuroscience 2020Quote: ... The Illumina adapters were completed during a library amplification step and the libraries were quantified using either the BioPhotometer (Eppendorf) or Pherastar (BMG Labtech) ...
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bioRxiv - Microbiology 2019Quote: ... The qPCR reactions were performed in duplicate and in two independent runs using MasterCycler realplex4 from Eppendorf. Statistical analysis is described in the section below.
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bioRxiv - Systems Biology 2022Quote: ... The polar and the non-polar phase were transferred into two new and separate reaction vials (Eppendorf low binding tube ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ug of RNA was reverse transcribed into cDNA using the qScript XLT cDNA Supermix (QuantaBio, 95048, Beverly, MA) in the Eppendorf Mastercycler (Eppendorf, Hauppauge, NY) under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... two milliliters of the fermentation broth containing the suspended mucin beads were sampled to 2-ml tubes (Eppendorf). The serum bottles were opened and closed carefully each time to avoid contamination in the anaerobic workstation ...
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bioRxiv - Biochemistry 2023Quote: ... Two extracts from the same sample were combined and dried by Vacufuge Concentrator System (Eppendorf 5301, Eppendorf, Germany).
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bioRxiv - Biochemistry 2023Quote: ... Two extracts from the same sample were combined and dried by Vacufuge Concentrator System (Eppendorf 5301, Eppendorf, Germany).
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bioRxiv - Genomics 2022Quote: ... The cells were then centrifuged at 21,130 x g for two minutes at room temperature in a benchtop microcentrifuge (model number 5424, Eppendorf). The supernatant was decanted by aspiration and the cells were resuspended in 300 μL of a buffer consisting of 20 mM Tris-HCl (Fisher BioReagent part number BP2475-500) ...
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bioRxiv - Physiology 2024Quote: ... and the SCN was punched out of the two slices and immediately transferred to a pre-frozen 1.5 ml microtube (Eppendorf) kept on dryice ...
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bioRxiv - Molecular Biology 2022Quote: ... The sRNA libraries were then amplified with PCR (Eppendorf, Germany), purified ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA was converted to cDNA on an Eppendorf Mastercycler Pro (Eppendorf) and using Promega reagents ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
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bioRxiv - Microbiology 2020Quote: ... Newly emerged EVV-1 free EvaBx adults were CO2 anaesthetized and injected with 0.5 μL of the virus-containing extract between two abdominal segments using a stereomicroscope with a Cell Tram Oil microinjector (Eppendorf). As negative control in order to exclude any procedural contamination ...
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bioRxiv - Microbiology 2020Quote: Newly-emerged adults were anaesthetized with CO2 and microinjected between two abdominal segments under a stereomicroscope using a fine glass needle connected to a Cell Tram Oil microinjector (Eppendorf). Insects were microinjected with 0.5 μL of dsRNAs at the concentration of 160 ng μL-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Unincorporated biotin-HPDP was removed by two rounds of chloroform extraction with 2 ml Phase lock gel heavy tubes (Eppendorf). RNA was precipitated with 1/10 volume of 5M NaCl and an equal volume of Isopropanol ...
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bioRxiv - Genetics 2022Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passes (1 + 17, 2 + 18 … etc.) and dried in a Speed-Vac (Eppendorf).
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for two hours with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions were collected every three minutes, pooled in two passes (fraction 1 + 17, fraction 2 + 18,…, etc.) and dried in a vacuum centrifuge (Eppendorf).
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bioRxiv - Cell Biology 2022Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passed (1 + 17, 2 + 18 … etc.) and dried in a vacuum centrifuge (Eppendorf). Dried fractions were resuspended in 0.1% formic acid and analyzed on a Orbitrap Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passes (1 + 17, 2 + 18 … etc.) and dried in a vacuum centrifuge (Eppendorf). Dried fractions were re-suspended in 0.1% formic acid (FA ...
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bioRxiv - Evolutionary Biology 2021Quote: The pooled libraries were dried in a SpinVac (Eppendorf, Dusseldorf, Germany), resuspended in 8 µl of 10 mM Tris (pH 8.0 ...
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bioRxiv - Systems Biology 2022Quote: ... The polar and the non-polar phase were transferred into two new and separate reaction vials (Eppendorf low binding tube, 1.5 mL, Eppendorf, Hamburg, Germany), evaporated to dryness using an Eppendorf Concentrator Plus (Eppendorf ...
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bioRxiv - Pathology 2021Quote: ... The resulting cDNA was stored in LoBind DNA Eppendorf tubes (Eppendorf, 0030108051) at −20°C ...
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bioRxiv - Genomics 2021Quote: ... 2 uL of cDNA were amplified in a Mastercycler Gradient instrument (Eppendorf) in 100 uL total reaction volume using Phusion high fidelity polymerase (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: ... One microliter of each cDNA sample was used for TaqMan PCR (Eppendorf RealPlex Mastercycler and Applied Biosystems QuantStudio 3 ...
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bioRxiv - Microbiology 2022Quote: Ninety microliters of bacterial suspension were mixed with an appropriate volume of two-fold dilutions of gliotoxin and placed into wells of a 96-wells microplate with non-transparent side walls (Eppendorf, Hamburg, Germany) up to the final volume of 100 μL.
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bioRxiv - Molecular Biology 2020Quote: ... Barcoded cDNA was then pooled in 2 mL DNA LoBind tubes (Eppendorf #0030108051) and cleaned up using AMPure XP beads at a ratio of 0.5X volume ...
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bioRxiv - Cell Biology 2019Quote: ... mRNA was converted to cDNA on an Eppendorf Mastercycler Pro (Eppendorf, Hamburg, Germany) and using Promega reagents ...
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bioRxiv - Genomics 2021Quote: ... RNA and cDNA purity was measured using a Biospectrometer (Eppendorf AG, Hamburg, Germany) and the concentrations were quantified using PicoGreen Quant-iT dsDNA quantitation assay (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... All Library preparations were performed on twin.tec PCR Plates LoBind 250 μL (Eppendorf). Library quantitation was performed with Qubit dsDNA HS kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... EMBL Heidelberg for library preparation and sequencing in low bind RNA tubes (Eppendorf).
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bioRxiv - Neuroscience 2021Quote: ... The cDNA was purified using an automated liquid handling robot (epMotion 5057; Eppendorf, USA), and the library complexity was confirmed by sequencing 10% of selected colonies.
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bioRxiv - Cancer Biology 2022Quote: ... was used to convert the RNA to cDNA via a thermocycler (Eppendorf, Hamburg, Germany). The PCR conditions were as follow ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries were validated via qPCR on a Mastercycler ep realplex (Eppendorf AG, Hamburg, Germany) using the KAPA library quantification kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... The pooled DNA library was concentrated with an Eppendorf Concentrator plus (Eppendorf AG, Hamburg, Germany) and sequenced on an Illumina Miseq Sequencer by a Eurofins Scientific SE.
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bioRxiv - Synthetic Biology 2024Quote: ... Both sequencing libraries were prepared on an epMotion 5075 TC liquid handler (Eppendorf, Hamburg, DE) using the ILMN DNA LP (M ...
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bioRxiv - Immunology 2019Quote: ... ∼10 ng of cDNA was stamped into a fresh 384-well plate (Eppendorf, Cat. No. 0030128508) for sequencing library preparation.