Labshake search
Citations for Eppendorf :
351 - 400 of 487 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... cells were pelleted by centrifugation for 5 min at 15,700 x g using 5415R Microcentrifuge (Eppendorf, Hamburg, Germany) and the supernatant was frozen at −20° C until further use ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 mL subsamples of cell cultures normalized as above were dispensed into 2 mL tubes (cat: 0030120094, Eppendorf) and stained with MitoTracker Orange CMTMRos (cat ...
-
bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 (68) were cultured in human red blood cells (O+ or B+, Blood bank, Universitätsklinikum Hamburg-Eppendorf). Cultures were maintained at 37°C in an atmosphere of 1% O2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by shaking at 1,400 rpm for 30 min at 65°C in a Thermomixer (Eppendorf) followed by centrifugation for 5 min at 13,000 g ...
-
bioRxiv - Microbiology 2023Quote: ... we centrifuged 1 mL overnight-cell culture in a 1.5 mL Eppendorf tube at 7’500 rcf for 5 min (Eppendorf, tabletop centrifuge MiniSpin plus with rotor F-45-12-11 ...
-
bioRxiv - Microbiology 2023Quote: ... and the cells were incubated at 37°C/ 5% CO2/ 1% O2 (Galaxy 170 R/ New Brunswick/ Eppendorf). Cells were cultured for up to 14 days with bi-weekly media change ...
-
bioRxiv - Genomics 2024Quote: ... parvum cells that were isolated using a micromanipulator (MMO-202ND; Narishige) equipped with a microinjector (CellTramm Oil; Eppendorf) that held a capillary (Figure 1A) ...
-
bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was first cleared by centrifugation at 1400 x g for 15 min (5920R; Eppendorf) and then filtered through a 0.45-µm PES filter (Merck Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... pH 7.6) and injected into 1 to 2-cell-stage embryos by using a microinjector FemtoJet 5247 (Eppendorf). The sequences and the concentrations of MOs (Gene Tools ...
-
bioRxiv - Cell Biology 2020Quote: ... and cell debris was spun down at 20,000xg for 10 minutes at 4°C using a 5810 R Centrifuge (Eppendorf). Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... and injected into the nucleus of HeLa or U-2 OS cells using an Eppendorf FemtoJet® Microinjector (Eppendorf). After media were changed ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were sorted using a 100 um nozzle (∼20 PSI) into 2 ml protein LoBind Eppendorf tubes (Eppendorf 0030108132) placed in a cooling holder ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were harvested by centrifugation (3200 g, 40 min, 4 °C; Eppendorf centrifuge 5810 R, Eppendorf AG, Hamburg, Germany), and the cell pellets were stored (−20 °C) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1% P/S on a magnetic separator cells were cultured in fibronectin-coated 6-well plates (Eppendorf, Hamburg, Germany) in DMEM/F-12 ...
-
bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
-
bioRxiv - Bioengineering 2021Quote: ... MNPs were injected into cells using a micro manipulation system (Inject-Man 4 and FemtoJet 4i, Eppendorf, Hamburg, Germany) with microinjection capillaries (Femtotip II ...
-
bioRxiv - Systems Biology 2021Quote: ... Cell lysates were transferred to a new tube and were centrifuged for 2 min at full speed (Eppendorf centrifuge) and the supernatant was carefully mixed with 1 volume of 70% HPLC-grade ethanol ...
-
bioRxiv - Plant Biology 2021Quote: ... were made from single colonies of putative Xcm strains and cell suspensions (optical density (OD) = 0.3) were generated in MilliQ ultrapure H2O using a spectrophotometer (Eppendorf BioSpectrometer basic ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Cells were then pelleted by centrifugation at 3900 rcf for 15 min (Eppendorf S5810R with S-4-104 rotor) and stored at -80 °C.
-
bioRxiv - Genomics 2019Quote: ... Cells were thawed and pelleted in a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Cell pellets were resuspended in 250 μl nuclei permeabilization buffer (10 mM Tris-HCl pH 7.4 (Sigma) ...
-
bioRxiv - Cancer Biology 2019Quote: ... treated cells washed and scrapped in chilled PBS followed by pop-spin for 10 sec in tabletop centrifuge (Eppendorf). After discarding supernatants ...
-
bioRxiv - Cell Biology 2019Quote: ... Approximately 5– 10 nl of cell suspension was microinjected into the pericardial cavity of the embryo using CellTramVario (Eppendorf), Injectman Ni2 (Eppendorf ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
-
bioRxiv - Microbiology 2020Quote: ... were obtained by removing the head of a CO2-anaesthetized adult and drawing 0.5 μL hemolymph from the inner thorax cavity with a Cell Tram Oil microinjector (Eppendorf), using a stereomicroscope ...
-
bioRxiv - Microbiology 2021Quote: ... cells equivalent to 1 OD600 were pelleted by centrifugation at 2,808 x g for 10 min (Eppendorf 5810 R) and the pellets were flash frozen ...
-
bioRxiv - Neuroscience 2020Quote: ... washed twice with 160 µL PBS and nearly 40,000 cells per well transferred onto the membrane and dried with a plate thermomixer (Eppendorf) at 50 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... after which the mixture was filtered using a 100 µm cell strainer in to a 1.5 ml tube (Eppendorf). The mixture was then transferred to a clean 1 ml dounce homogenizer to which 750 ul of buffer A containing 1% Igepal (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: Oocyte and 1-cell embryo microinjections were performed using a Femtojet injector and TransferMan 4r micromanipulators (Eppendorf, Enfield, CT) on a Leica DMI 6000B inverted microscope ...
-
bioRxiv - Molecular Biology 2022Quote: ... the resulting cell lysates were passed through a 20 G needle 100 times or heated using a Thermomixer (Eppendorf) at 55°C for 10 minutes with 1000 rpm agitation prior to the conventional RNA purification procedure in accordance with the manufacturer’s protocol.
-
bioRxiv - Microbiology 2023Quote: ... supernatant of infected cells was collected and centrifuged for 2.5 min at 25200×g in a refrigerated micro-centrifuge (Eppendorf). Lactate dehydrogenase (LDH ...
-
bioRxiv - Cell Biology 2022Quote: Cells were seeded at a density of 0.4 x105 cells onto coverslips (Blue Star) in 24-well petri plates (Eppendorf). After appropriate drug treatment ...
-
bioRxiv - Immunology 2023Quote: ... 50 mL of diluted bacterial suspensions were seeded onto a 96-well glass-bottom plates (Cell imaging plate, Eppendorf) and incubated for 30 min at 37°C ...
-
bioRxiv - Genomics 2024Quote: ... the 1*105 cells were taken off for ATAC sequencing and placed into DNA LoBind 1.5mL tubes (Eppendorf, 2231000945). The cells were washed 3x in ice cold buffered natrium chloride (PBS ...
-
bioRxiv - Immunology 2023Quote: ... Cells were directly FACS sorted without an intermediate MACS column enrichment step using FACSAria Fusion Cell Sorter (Becton Dickinson) in LoBind 1.5 mL microcentrifuge (Eppendorf) tubes with 1:1 FBS:PBS supplemented with recombinant RNase inhibitor (1:100 ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was used for microinjection into adherent HeLa-GFPvim cells with a microinjection system (Injectman II and Femtojet, Eppendorf), and injection parameters were 100 Pa injection pressure ...
-
bioRxiv - Cell Biology 2019Quote: ... HeLa SS6 or 1.E cells were seeded into 35 mm tissue culture dishes or 6 well tissue culture plates (Eppendorf) with or without pre-cleaned ...
-
bioRxiv - Genomics 2021Quote: ... live cell was index-sorted into one 96-well quadrant of a 384-well plate (Eppendorf lo-bind twin-tec) containing a mixture of DPBS and shearing master mix at a final volume of 2.14 μL using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter) ...
-
bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
-
bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
-
bioRxiv - Microbiology 2021Quote: ... and a volume corresponding to an OD600nm of one (109 cells) was pelleted at 14,000 x g for one minute (Eppendorf MiniSpin). 20 µl of supernatant was mixed with 50 µl BioLux Gaussia Luciferase Assay substrate (New England Biolabs ...
-
bioRxiv - Cell Biology 2021Quote: Single osteoblastic cells were stimulated by local membrane indentation with a glass micropipette using a FemtoJet microinjector NI2 (Eppendorf Inc.), as previously described (Mikolajewicz ...
-
bioRxiv - Cell Biology 2021Quote: ... ATP and ADP levels were normalized for an optical density at 600 nm of the initial cell suspension assessed by BioSpectrometer (Eppendorf).
-
bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
-
Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
-
bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
-
bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells from each brain region were sorted in a separate A1 well of a 96 well PCR plate (Eppendorf, 951020401) containing 100 µl of PBS buffer with 0.3% BSA ...
-
bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...