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Citations for Eppendorf :
51 - 100 of 366 citations for S + α 1 Naphthyl ethylamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization between the target mRNA and reporter-capture probe pairs was performed for 18 hours at 65°C using Mastercycler Pro S Thermal Cycler (Eppendorf) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 96-well plate was sealed with a sterile and optically transparent sealing film (PCR-SP-S, AxySeal Scientific) and covered with a polystyrene plate lid (Eppendorf) that had silicone grease lining the edges ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was performed with the realplex2 Mastercycler epgradient S automated real-time PCR system (Eppendorf®, New York City, NY) with standard reaction conditions (Table S8) ...
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bioRxiv - Biochemistry 2023Quote: ... The assay plates were covered with a self-adhesive aluminium foil and incubated at 30°C for the indicated time point(s) in an Eppendorf ThermoMixer C (Eppendorf) equipped with a ThermoTop 288 and a SmartBlock™ PCR 384 ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Cell Biology 2023Quote: ... is harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore ...
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bioRxiv - Physiology 2020Quote: MIN6 and α-TC9 cells were seeded at 104 cells/well in a 96-well cell imaging plate (Eppendorf, 0030741013) and transduced the following day with the ratiometric cilia-targeted cADDis BacMam (Molecular Montana ...
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bioRxiv - Neuroscience 2023Quote: ... insoluble PFF were produced by incubation of 300 µM recombinant α-syn in a LoBind reaction tube (Eppendorf GmbH, GE) with one borosilicate glass bead (d = 3.0 mm ...
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bioRxiv - Bioengineering 2020Quote: ... the gel was then flushed twice with 100 µL of warm medium at 187.5 µL/s flow (Eppendorf Xplorer automatic pipet), generating a 3D-ECM on the membrane with a thickness of 30-50 µm (data not shown) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was then centrifuged at 1000 rpm for 30 s (5804/ 5804 R – Benchtop Centrifuge, Eppendorf North America, CT, USA), mixed on a plate shaker (Siemens DPC MicroMix 5 ...
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bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
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bioRxiv - Evolutionary Biology 2019Quote: ... pre-loaded with 70µl/well of bacterized CM and incubated at 43°C for 1.5 min in a thermocycler (Eppendorf Mastercycler® pro S). Cells were randomly assigned to plate wells for both control (no heat shock ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2022Quote: ... the gel was then flushed twice with 100 µL of warm medium at 187.5 µL/s flow (Eppendorf Xplorer automatic pipet; Eppendorf). This generated a 3D-ECM layer on the membrane with a thickness of 20-85 µm ...
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bioRxiv - Neuroscience 2023Quote: ... S-opsin-positive cells (S-cones) and negative cells with cone morphology (M-cones) were separately collected using a micromanipulator (Eppendorf TransferMan 4r) and transferred into 8 µL lysis buffer (Clontech SMARTer Ultra Low Input RNA Kit for Sequencing - v3) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Thermo Fisher Scientific except the monoclonal α-AMP antibody which is a generous gift of Aymelt Itzen (University Medical Center Hamburg Eppendorf, Germany). Unless otherwise stated ...
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bioRxiv - Developmental Biology 2019Quote: ... The embryos were deyolked in ice cold ringer’s solution using micropipette tip and spun at 100g for 2 minutes at cooled table top centrifuge at 40C (Eppendorf® centrifuge 5418R). The supernatant was discarded and the embryo bodies were trypsinized using TrypLE Express (Thermoscientific® ...
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bioRxiv - Genetics 2021Quote: ... 72°C 45 s and a final extension at 72°C for 5 min was run on an Eppendorf Mastercycler® (Eppendorf AG, Hamburg Germany). Amplicons were observed on a 1.5% agarose gel (Bioline (Aust ...
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bioRxiv - Microbiology 2019Quote: ... four 50 mL aliquots from each trap were centrifuged at 3214 x g and 4°C for 30 minutes (Eppendorf 5810 R, S-4-104 rotor). The supernatant was decanted after centrifugation and cell pellets were stored at −80°C until ready for freeze-drying and lipid extraction ...
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bioRxiv - Biophysics 2021Quote: ... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
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bioRxiv - Immunology 2019Quote: ... and concentrated to ~1 μg μl-1 using a Concentrator 5301 (Eppendorf).
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bioRxiv - Biochemistry 2021Quote: ... diluted 1:1 with F-actin buffer and cleared by centrifugation (Eppendorf centrifuge 5424R ...
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bioRxiv - Biophysics 2021Quote: ... We put 500 mL of log phase density (1×106-1×107 cells/mL) cells into 1 mL disposable cuvettes (UVette, 952010051, Eppendorf AG, Hamburg, Germany). We measured the OD600 every hour for 7 hours in each of two cuvettes for each cell line ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × Denhardt’s solution (Eppendorf, 0032007.155). Hybridization was performed with a double DIG-labeled locked nucleic acid (LNA ...
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bioRxiv - Microbiology 2022Quote: ... 1% oxygen (hypoxic; Eppendorf CellXpert incubator), or 0% oxygen (anoxic ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The sequencing failed for 1 sample and 1 sample was damaged during the travel (Eppendorf tube burst), decreasing our total sample size to 41 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...
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bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Developmental Biology 2021Quote: ... A Femtotip microcapillary (1 μm tip diameter, Eppendorf, Germany) was mounted onto a Femtojet pump and micromanipulator (Eppendorf) ...
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bioRxiv - Biochemistry 2019Quote: ... Dilutions (1:2) were performed using EP Motion (Eppendorf) and transferred to cells using the Tecan Freedom Evo ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were washed twice for 3 min at 400 RCF at room temperature with PBS supplemented with 1% glucose and 1% pen-strep (Eppendorf 5702R, Hamburg, Germany). Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... and agitated (1,300rpm, 30 min, 1°C; Thermomixer Comfort, Eppendorf). Solubilized membrane proteins were obtained in the supernatant from this agitated suspension by centrifugation (21,000g ...
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bioRxiv - Immunology 2020Quote: ... The pH of the eluate (1 mL per Eppendorf tube) was neutralized (125 µL of Tris 1M ...
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bioRxiv - Immunology 2019Quote: ... for 1 hour at 37°C in a Thermomixer (Eppendorf) with shaking set at 1000rpm ...
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bioRxiv - Microbiology 2020Quote: ... The remaining 1 mL of culture was centrifuged (Eppendorf 5417R) at 8000xg and washed twice with 1 mL of LB media ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 ml aliquots were concentrated in a speed-vac (Eppendorf) for 45 min at 30°C ...
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bioRxiv - Physiology 2023Quote: ... for 1 h at 4 □ on a ThermoMixer C (Eppendorf), 350 µl of water and 250 µl of chloroform were added to the mixture to induce phase separation ...
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bioRxiv - Physiology 2020Quote: ... 1% Antibiotic-Antimycotic] in a 5% CO2 incubator (Galaxy 170R, Eppendorf) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... bacterial cultures (1 mL) were separately transferred to sterile cuvettes (Eppendorf), and the optical density (OD600 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by centrifugation (1000 rpm, 1 minute, Eppendorf 5804R, Hamburg, Germany) and subsequently the plates were incubated for 30 minutes at room temperature (RT) ...
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bioRxiv - Molecular Biology 2022Quote: ... at 211 g for 1 hour at room temperature (Eppendorf ThermoMixerC). Eluate was then removed ...
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bioRxiv - Molecular Biology 2021Quote: ... and spun down at 2,000 rcf for 1 minute (Eppendorf 5810R) before stored at −80 °C.