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Citations for Eppendorf :
651 - 700 of 1087 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Biochemistry 2021Quote: ... 33 mg of MFC tissue stored at −80°C was transferred to 1.5 mL LoBind Eppendorf tubes (Eppendorf, Cat# 022431081) and combined with 0.5 mL homogenization buffer consisting of 8 M urea ...
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bioRxiv - Biochemistry 2021Quote: ... at a molar ratio crosslinker to lysines of ∼2.7 for 30 min (except for the time-course experiments, see below) at 37°C shaking at 650 rpm in a Thermomixer (Eppendorf). Protein samples were quenched by addition of ammonium bicarbonate to a final concentration of 50 mM and either directly evaporated to dryness or after an additional centrifugation step for 60 min at 21 000 x g in order to separate the condensed from the dilute phase ...
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bioRxiv - Biochemistry 2020Quote: ... Cultures were grown with shaking at 200 rpm at 30 °C on an Innova 2300 platform shaker (Eppendorf, Hamburg, Germany) to maximal culture density ...
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bioRxiv - Bioengineering 2021Quote: ... a 96-well plate pre-filled with 20 μL pure water and sorted with designated cells per well was frozen on dry ice for 5 min and heated by ThermoMixer C (Eppendorf) at 95 °C for 10 min followed by spinning down at 3000 rpm for 1 min ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Plant Biology 2021Quote: ... samples were derivatized with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 1.0 mg per sample, pH 4.0, 60 min, 37 °C under continuous shaking in an Eppendorf thermomixer) and analysed using a UPLC-MS/MS equipped with an electrospray interface in positive mode (ACQUITY ...
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bioRxiv - Microbiology 2021Quote: ... The dialysis block was then incubated at 37°C for 6 hours with constant shaking at 400rpm (Thermomixer comfort, Eppendorf). After 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in a test tube at 30°C and optical density at 600 nm (OD600) was recorded using a spectrophotometer (Eppendorf). To obtain doubling time ...
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bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... Hybridization between the target mRNA and reporter-capture probe pairs was performed for 18 hours at 65°C using Mastercycler Pro S Thermal Cycler (Eppendorf) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37°C and 5% CO2 in a humidified incubator (Galaxy 170S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Bioengineering 2022Quote: ... tumors were minced using a razor and digested with 1mg/ml collagenase A and collagenase D and 0.4mg/ml DNase I in PBS at 37°C for 2h with rotation at 600rpm in a thermomixer compact (Eppendorf). 10mM EDTA was then added to stop the enzymatic reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were kept at 37 °C and 5% CO2 in a Galaxy 170S CO2 humidified incubator (Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80%.
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bioRxiv - Biochemistry 2022Quote: ... Peptides purified via the SDB-RPS approach were dried at 45 °C in a centrifugal vacuum concentrator (Concentrator 5301, Eppendorf), resuspended in buffer A* (0.1 % [v/v] TFA ...
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bioRxiv - Cell Biology 2022Quote: ... then with 150 µl of 100% acetonitrile to the gel pieces for 15 min at 25°C while being agitated at 1400 rpm in a ThermoMixer (Eppendorf). Extracted peptides combined ...
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Microbiology 2021Quote: ... with 100 μl aliquot of the ultra-low gelling temperature agarose gel solution (6% in Mueller Hinton Broth II, cation-adjusted) to 85 °C in a Thermomixer (Eppendorf Thermomixer R Shaker, Eppendorf). Once the temperature was reached ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Microbiology 2020Quote: ... The 20 mL cultures used for all other analyses were incubated at 37°C in an Innova 40R shaking incubator (Eppendorf) at 230 rpm until they reached an OD600 of 0.7.
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bioRxiv - Neuroscience 2021Quote: ... Sarkospin solubilization was then performed by incubating the samples at 37 °C under constant shaking at 600 rpm (Thermomixer, Eppendorf) for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were digested with trypsin (1:50 trypsin:protein ratio) overnight at 37°C with light agitation (450 rpm on a Eppendorf thermomixer). Digestion was stopped by the addition of trifluoracetic acid (TFA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Homogenates were centrifuged at 1000 rpm for 5 min at 4°C in a table centrifuge (5424 R, Eppendorf, Germany). The supernatant was transferred to a new tube and again centrifuged at 4°C for 5 min at 1400 rpm ...
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bioRxiv - Genetics 2022Quote: ... The samples were then incubated at room temperature (about 25°C) at 450 rpm for 20 mins on Thermomixer (Eppendorf). Putting the samples on magnetic stand to facilitate removing supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... 100 U/ml DNase I in HBSS−/−) at 37°C for 20 minutes with constant agitation (300 RPM) in a ThermoMixer (Eppendorf). All subsequent steps were performed as previously described9,14 ...
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bioRxiv - Systems Biology 2019Quote: ... One of the fermentations was performed in CDM at 37°C in 3.0 L bioreactors (BioFlo 115, New Brunswick Scientific/Eppendorf) with a 2.2 L working volume ...
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bioRxiv - Microbiology 2019Quote: ... proteinase K (50mg/μl) was added and the samples were incubated overnight at 56°C at 600 rpm (Thermomix comfort, Eppendorf). Appropriate amounts of AL buffer (Qiagen GmbH ...
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bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Bioengineering 2019Quote: ... the crude extracts were transferred into a fresh tube and centrifuged for 30 min at 15000g and 4 °C (Centrifuge 5427R, Eppendorf). The soluble supernatant (blue color ...
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bioRxiv - Biophysics 2019Quote: ... Samples were then incubated for 30 min at 45°C and 500 rpm using ThermoMixer® (Eppendorf, Wesseling/Berzdorf, Germany). After incubation ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation reactions were carried out for the indicated proteins using rabbit reticulocyte lysate and SP cells at a final concentration of 3×106 cells/ml at 30°C for 40 min in a thermomixer (Eppendorf) set to 900 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with 400 μL ChIP elution buffer (containing 50 μg RNase A) and incubated at 37 °C at 1,200 rpm on a Thermomixer (Eppendorf, Germany) for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred to a new tube and solvents were removed by evaporation at 30°C for 90 min at reduced pressure (Concentrator Plus, Eppendorf). The samples were then fully dried in a freeze dryer and stored at −80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sorted nuclei were then centrifuged at 1000 g for 15 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3), and supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... in 200 µl volumes grown at 37 °C with 200 rpm shaking in an Innova 42R Incubator (Eppendorf, United Kingdom) for 22 h growth periods (‘days’) ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for thirty minutes with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for two hours with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was diluted with RNAse free water to a concentration of 500ng/20ul and denatured at 70°C for 15min in a Mastercycler (Eppendorf). Mastermix was prepared by mixing ...