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Citations for Eppendorf :
101 - 142 of 142 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The crude cell lysate was refined by centrifuging at 18514 g for 40 min (Fixed angle rotor, 5810-R Centrifuge, Eppendorf). The clarified lysate was applied to a 5 mL HisTrap™TALON™ crude cobalt column (Cytiva ...
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bioRxiv - Evolutionary Biology 2022Quote: ... plated onto glass coverslips in 35-mm tissue culture-treated dishes (Eppendorf, Mississauga Ontario), and then left to adhere for a minimum of 6 hours before recording.
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bioRxiv - Evolutionary Biology 2021Quote: ... We agitated the tube containing tissue and media at 300rpm (ThermoMixer F1.5, Eppendorf, Germany) at 37°C for ten minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Homogenised tissue was then transferred to 15 mL protein LoBind® tubes (Eppendorf, 0030122216), placed on a roller for 30 minutes at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Cancer Biology 2021Quote: ... 3-6 pooled tissue biopsies were moved into a precooled 1.5 mL tube (Eppendorf, Germany) containing 300µL digestion cocktail consisting of Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of freeze-dried tissue was transferred to 2-ml microcentrifuge tubes (Eppendorf Safe-Lock tubes ...
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bioRxiv - Microbiology 2021Quote: ... Organ samples were homogenized in 1 ml cell culture media using tissue homogenizer (Eppendorf, Germany). MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit was used for RNA extraction as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the pre-weighed mouse tissues were transferred to Protein LoBind 1.5 mL microcentrifuge tubes (Eppendorf) using 500 µL cold sterile DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
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bioRxiv - Biochemistry 2019Quote: ... the tissue samples were centrifuged at 300 × g for 10 min at 4°C (# 5720R Eppendorf). The supernatant from 300 × g was transferred to new 15mL tube and then centrifuged at 2,000 × g for 10 min at 4°C (# 5720R Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... and tissue punches of the regions were collected into 1.5ml DNA LoBind tubes (Eppendorf, Hamburg, Germany). The punches were then snap-frozen using dry ice before being stored at -80 C° until RNA isolation.
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were incubated for 30 min at 37°C and 950x RPM on a Thermoblock (Eppendorf, Germany) and mechanically dissociated every 10 min with a pipette ...
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bioRxiv - Physiology 2024Quote: ... 125 ng/ml BIS-TRIS solution were added per 50mg of tissue in 2ml Safe-Lock tubes (Eppendorf); one 3mm Tungsten carbide bead (69997 ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: Approximately 20 or 30 mg of leaf tissue was excised from fresh leaves and placed in a 1.5-mL tube (Safe-Lock, Eppendorf) with 600 or 900 µL of 85% MeOH warmed to approximately 68 °C ...
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bioRxiv - Zoology 2020Quote: ... The tissue samples were separately placed in 400 µl of 10% Chelex solution in a 1.5 mL tube (Eppendorf) and crushed using a pestle (Eppendorf) ...
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bioRxiv - Genomics 2024Quote: ... 30 mg of the frozen tissue consisting of several fronds was weighed into 1.5 ml tubes (Eppendorf, Hamburg, Germany) with two 3 mm metal beads and ground into a fine powder with a TissueLyser II (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... using the enzymatic cleanup tissue extraction protocol with proteinase K and RNase A and quantified using a BioSpectrometer (Eppendorf). All other samples were extracted using the BioSprint (QIAGEN ...
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bioRxiv - Bioengineering 2019Quote: ... EBs were harvest according to the manufacturer protocol and transferred to a 10 cm tissue culture dish (Eppendorf, catalog 0030702018). On Day 5 ...
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bioRxiv - Biochemistry 2021Quote: ... 33 mg of MFC tissue stored at −80°C was transferred to 1.5 mL LoBind Eppendorf tubes (Eppendorf, Cat# 022431081) and combined with 0.5 mL homogenization buffer consisting of 8 M urea ...
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bioRxiv - Neuroscience 2020Quote: ... Live tissue slices were mounted onto the coverglass bottom surface of 35mm cell imaging dishes (Eppendorf, 145 μm thickness, 0030740009) coated with Cell-Tak Cell and Tissue Adhesive (Corning™ ...
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bioRxiv - Immunology 2021Quote: ... at increasing concentrations of 3.2 pg/mL–50 ng/mL per well in a 96-well tissue culture plate (Eppendorf, #30730127). LPS is a potent Toll-like receptor (TLR ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Immunology 2022Quote: 500 μL of TRISURE reagent was added to each frozen tissue and these were dissociated in an RNAse free microcentrifuge tube (Eppendorf) using a homogenisation pestle ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were fixed in 2% paraformaldehyde for 55 minutes at room temperature in 2 mL Protein LoBind tubes (Eppendorf 022431064). Fixative was removed and tissues were washed 4x 10 minutes with 1.5 mL PBS with 0.5% Triton X-100 (PBT) ...
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bioRxiv - Cell Biology 2023Quote: Approximately 0.04 g of fresh or frozen tissue was minced using a blade and collected into a 1.5-mL DNA LoBind® Tube (Eppendorf), to which 1 mL of PBS was added.
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bioRxiv - Cancer Biology 2023Quote: 20-30 mg sections of snap frozen zebrafish liver tissue were transferred to pre-chilled Safe-Lock tubes (Eppendorf, 022363352) containing a cold 5/16 in ...
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bioRxiv - Neuroscience 2024Quote: ... and snap-frozen by placing the tissue in pre-chilled low bind 2 mL microfuge tubes on dry ice (#022431048 Eppendorf). FC sections (male n = 27 ...
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bioRxiv - Systems Biology 2021Quote: Cell culture (nuclei or whole cells) and tissue samples were collected by automated laser microdissection into 384-well plates (Eppendorf 0030129547). For the collection of different U2OS nuclei classes (Fig ...
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of freeze-dried tissue was transferred to 2-ml microcentrifuge tubes (Eppendorf Safe-Lock tubes; Eppendorf, Hamburg, Germany) containing a single 4-mm stainless-steel grinding ball (SPEX SamplePrep ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred all the tissue fragments from the Petri dish into a clean 5 mL low-binding tube (Eppendorf, cat. no. 0030108.310), and rotated the tube at 20 rpm at room temperature for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... The cells stably expressing H-Luc-NLS were plated on a black flat glass bottom 96-well plates (Eppendorf; tissue culture treated). They were incubated with 1 μM MaPCahigh AM dyes and 0.04% Pluronic-F127 for at least 2 h in 100 μL at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Microbiology 2019Quote: ... Pupae were aligned on double-sided sticky tape and injected in the third last segment at the posterior end close to germinal tissue using a FemtoJet microinjector system (Eppendorf, NSW, Australia). The microinjected pupae were then placed on moist Whatman filter paper and incubated at 27°C until flies emerged ...
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bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
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bioRxiv - Bioengineering 2023Quote: ... Transfected cells were transferred into 8.0 mL of MA2 media in 20 mL culture tubes and allowed to recover while rotating (∼80 rpm) in the outer rim of a tissue culture roller drum (New Brunswick; model TC-7; Eppendorf, USA) housed in an Algatron® incubator (Photon Systems Instruments ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of brain tissue or BDEV lipid extracts were aliquoted in triplicate to individual wells of a twin-tec® 96-well plate (Eppendorf, Hamburg, Germany). The brain tissue lipid extracts and BDEV lipid extract were dried and then resuspended in 50 μL (brain tissue ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) and in situ fixed and washed as described above ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...