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Citations for Eppendorf :
201 - 250 of 285 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... samples were sorted into PBS with 0.04% BSA (w/v) in 1.5 mL Protein LoBind tubes (Eppendorf, 0030108442). For ATAC-seq ...
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bioRxiv - Biochemistry 2023Quote: ... 380 μL samples of 8 μM aSyn solutions were incubated in Protein LoBind polypropylene tubes (Eppendorf, ref.0030108.116) at 37 °C under quiescent conditions over 10 days in the absence and presence of a cylindrical 10 mm x 3mm Teflon stirring bar (Merck ...
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bioRxiv - Plant Biology 2022Quote: ... The captured-on beads proteins were digested overnight at 37°C under vigorous shaking (1200 rpm, Eppendorf Thermomixer) with 1 ug Trypsin/LysC (MS grade ...
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bioRxiv - Plant Biology 2023Quote: ... The protein samples were centrifuged at maximum speed for 1 min in an Eppendorf™ 5424 Microcentrifuge (Eppendorf). The proteins were separated on 10% SDS polyacrylamide gels and transferred onto Immuno-Blot PVDF membrane (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... Protein fractions of interest were pooled and concentrated using an amicon of 10 kDa MWCO (Eppendorf 5804R, ∼3000g), before being loaded onto a size exclusion Superdex 75 HiLoad 16/600pg column (GE-Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein fractions of interest were pooled and concentrated using an amicon of 10 kDa MWCO (Eppendorf 5804R, ∼3000g) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and transferred them into 500 μL of the Schneider’s medium in 1.5 mL protein low-binding tubes (Eppendorf) on ice ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were sorted using a 100 um nozzle (∼20 PSI) into 2 ml protein LoBind Eppendorf tubes (Eppendorf 0030108132) placed in a cooling holder ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos at the desired stages were dechorionated in 0.5% sodium hypochlorite solution and collected in microtubes (Protein LoBind tubes, Eppendorf), which were cooled on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... conditioned media was collected and filtered through Spin-X 0.45 µM cellulose acetate centrifuge tube filter (#8163, Costar) and stored at −20°C in protein Lobind tubes (Eppendorf) until further usage ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were eluted in 40 μL of 0.2 M glycine pH 3 for 30 min on a ThermoMixer (Eppendorf) at 900 rpm at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells resuspended in the remaining supernatant were transferred to smaller tubes: first 5ml and then 1.5ml protein low binding microcentrifuge tubes (Eppendorf). After the final centrifugation step ...
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bioRxiv - Neuroscience 2021Quote: ... eggs were immediately collected and injected with a mixture of 6.25ng/ul Tol2 transposase protein and 10ng/ul plasmid DNA using a Femtojet (Eppendorf). Pulled glass capillaries were used for microinjection (WPI ...
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bioRxiv - Neuroscience 2022Quote: 30 asteroids per condition were pooled in a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Biochemistry 2019Quote: The HeLa cell and sorted cell samples were first lysed in 1.5ml protein Lobind tube (Eppendorf; cat. no. 022431081) with lysis buffer consisting of 1% sodium dodecyl sulphate (Fisher BioReagents ...
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bioRxiv - Neuroscience 2020Quote: ... ASyn proteins were incubated with or without shaking at 1000 rpm on a ThermoMixer (Eppendorf 5350, Hauppauge, NY, USA) at 37°C as indicated ...
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bioRxiv - Biophysics 2022Quote: ... The concentrations of the protein were calculated by ultraviolet (UV) absorbance at 280 nm using a UV spectrophotometer (Eppendorf), and the calculated molar extinction coefficients of 70N ...
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bioRxiv - Biophysics 2022Quote: ... The concentrations of the protein were calculated by ultraviolet (UV) absorbance at 280 nm using a UV spectrophotometer (Eppendorf), and the calculated molar extinction coefficients of HHD1 ...
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bioRxiv - Biophysics 2022Quote: ... Gel cubes corresponding to a single gel slice were transferred to a 1.5 mL microcentrifuge tube (Protein Lo-Bind, Eppendorf). 100 mM Ammonium Bicarbonate (ABC ...
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bioRxiv - Biochemistry 2023Quote: ... Protein LoBind® microtubes (2 mL) and epT.I.P.S.® pipette tips (1000 µL) were purchased from Eppendorf (Hamburg, Germany).
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bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
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bioRxiv - Genomics 2023Quote: ... eluted with 2 x 100 μl of 70 % (v/v) acetonitrile in 0.5 % (v/v) trifluoroacetic acid into low-binding microcentrifugation tubes (Protein LoBind, Eppendorf), and vacuum-dried in Vacufuge Concentrator Plus (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
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bioRxiv - Biochemistry 2023Quote: 20 µg of dry protein sample was dissolved in 40 µL 25% TFA in a 1.5mL low-binding polypropylene vial (Eppendorf). The vial was sealed with a micro tube cap lock (Scientific Specialties ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were washed with minimal washing buffer (50 mM Tris, pH 7.5; 150 mM NaCl) for 15 min and transferred to Protein LoBind tubes (Eppendorf). The washing procedure was repeated twice and beads were again transferred to new Protein LoBind tubes ...
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bioRxiv - Molecular Biology 2024Quote: A total of 25 fmol of Absolute Mag streptavidin nanomagnetic beads (CD Bioparticles: WHM-X047) were transferred to a 0.5 mL protein LoBind tube (Eppendorf) and mixed with 200 pmol of inner spacer module protein (biotin-3HB-SPYcatcher003 or biotin-60nm-SAH-SPYcatcher003 ...
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bioRxiv - Biochemistry 2023Quote: ... Polypropylene standard safe-lock microcentrifuge vials and corresponding low protein binding vials of 1.5 mL size from three manufacturers were used: Manufacturer A (Eppendorf Safe-Lock Tubes no ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were separated from the protein extracts on a magnetic rack and transferred to a new 2 ml LoBind tube (Eppendorf). The beads were washed with 1 ml of each following solution ...
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bioRxiv - Immunology 2021Quote: ... Equal volume of Mycobacteria tuberculosis H37RA-CFA was mixed with MOG/PBS in a 5 mL protein Lobind tube (Eppendorf), followed by emulsification in ice water through sonication ...
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bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
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bioRxiv - Biophysics 2021Quote: The gp15-gp16 core complex was obtained in vitro after incubation of the purified proteins for 30 min at room temperature (RT) in a thermo block with shaking (Eppendorf). The proteins were incubated using 180 pmol each at a 1:1 ratio ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Cancer Biology 2021Quote: ... 30-70 kDa lane portions were excised into 2×2 mm cubes and transferred to Protein Lo-Bind tubes (Eppendorf). Excised gels were partitioned into tubes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The fractions with highest concentrations (200–400 μg mL−1) were stored as 5–10 μL aliquots in Protein LoBind tubes (Eppendorf) at –80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Microbiology 2022Quote: ... the digested protein fraction was forced through the filter and collected in a 500 μl LoBind plate (Eppendorf, Hamburg, Germany). The filter was further washed with 100 μl 50 mM ABC followed by 100 μl H2O ...
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bioRxiv - Genomics 2022Quote: ... and the pellet was resuspended in 500 ul Buffer CT and the mixture was transferred to a 2.0 mL Protein LoBind tube (Eppendorf # 022431102). The suspension was spun again at 16,000 x g at 4 C for 2 min and the supernatant discarded ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (∼200 ul) was transferred to a 70uM filter (Fisher # NC1444112) set in a new 1.5 mL Protein LoBind tube (Eppendorf # 022431081). The tube with the 70 uM filter was spun on a mini-centrifuge (Ohaus # FC5306 ...
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bioRxiv - Immunology 2020Quote: ... plates were placed on magnetic plates and digestion reaction containing trypsinized peptides was transferred to a protein LoBind Eppendorf tube (Eppendorf, Cat ...
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bioRxiv - Biochemistry 2021Quote: ... and a concentration of 5 µg / ml of the plasmid with GFP (Green Fluorescent Protein) were transferred to an electroporation cuvette (2 mm wide and 400 µl in volume (Eppendorf)) ...
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bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of 200 µM monomeric αSyn was incubated in fibrillation buffer (Supplementary Table S2) in a 1.5 mL Protein LoBind Eppendorf tube (Eppendorf, Germany) on an orbital shaker (PCMT Thermoshaker Grant-bio ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were pelleted (600 x g, 2 min) and the supernatant was transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 2 × 300 μL HPLC grade water and the washes combined with the original supernatant ...
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bioRxiv - Synthetic Biology 2022Quote: Excitation and emission spectra of the fluorescent proteins were conducted using a BMG Clariostar Plus microplate reader in sealed plates (Corning, 3370; Eppendorf Masterclear real-time PCR film adhesive ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were fixed in 2% paraformaldehyde for 55 minutes at room temperature in 2 mL Protein LoBind tubes (Eppendorf 022431064). Fixative was removed and tissues were washed 4x 10 minutes with 1.5 mL PBS with 0.5% Triton X-100 (PBT) ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...