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Citations for Eppendorf :
51 - 100 of 131 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were allowed to proceed for 20 min (unless indicated otherwise) at 30°C in a ThermoMixer (Eppendorf) shaking at 550 rpm and were stopped by addition of 1xSDS loading buffer and boiling for 5 min at 95°C.
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bioRxiv - Immunology 2021Quote: ... The obtained lysates were centrifuged for 20 min at 21,000 g and 4°C (Centrifuge 5424 R, Eppendorf) to precipitate insoluble fractions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Extracts were incubated at −20°C for 1 hour and then centrifuged at 13,000 rpm (Eppendorf, Hauppauge, NY) and 4°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... and subjected to low-speed centrifugation at 20 × g for 5 min (Centrifuge 5810 R, Eppendorf, Hamburg, Germany) to eliminate gross particulate material ...
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bioRxiv - Biochemistry 2022Quote: ... 15 L of T-20052 (53) medium was prepared in a 20-liter Bioflow IV bioreactor (Eppendorf/NBS). The seed culture was collected and centrifuged at 3,000 x g for 10 minutes at 25¼;C ...
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bioRxiv - Molecular Biology 2023Quote: ... The final concentrate was supplemented with 0.1% Tween-20 and stored in a DNA LoBind microfuge tube (Eppendorf) in the fridge ...
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bioRxiv - Cancer Biology 2023Quote: ... Crude extracts were then clarified at 20,000*g for 20’ at 4C using a benchtop refrigerated microcentrifuge (Eppendorf). SDS-PAGE samples were prepared in NuPAGE LDS sample buffer and reducing agent ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were sorted using a 100 um nozzle (∼20 PSI) into 2 ml protein LoBind Eppendorf tubes (Eppendorf 0030108132) placed in a cooling holder ...
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bioRxiv - Microbiology 2019Quote: ... The bacteria were pelleted down by centrifugation at 3220 g and 20°C (in an Eppendorf Centrifuge 5810 R) before being resuspended in 10 times the original volume of MEM-Glutamax ...
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bioRxiv - Neuroscience 2020Quote: ... conditioned media was collected and filtered through Spin-X 0.45 µM cellulose acetate centrifuge tube filter (#8163, Costar) and stored at −20°C in protein Lobind tubes (Eppendorf) until further usage ...
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bioRxiv - Genetics 2020Quote: ... The slides were sealed and incubated for 20 min at 37 °C in a Thermoblock (ThermoMixer with Thermoblock, Eppendorf) with heated lid (ThermoTop ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture was incubated at 72 °C for 20 minutes in Mastercycler Pro 6321 (Eppendorf AG, Hamburg, Germany). End-modified PCR products were ligated into pGEM-T Easy vector (Promega ...
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bioRxiv - Genetics 2019Quote: ... and incubated for 6 days at 20°C while shaking at 200 rpm (New Brunswick I26/I26R shaker, Eppendorf). A stereomicroscope was used to assess developmental stage after 6 days incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... Elution buffer incubation time was extended to ~20 minutes and DNA was eluted into LoBind tubes (Eppendorf, Hamburg, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Samples were then centrifuged at 13,000×g for 20 min at 4 °C using an F2402 rotor in a GS15R table-top centrifuge (Eppendorf). Pellets were air dried ...
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bioRxiv - Molecular Biology 2022Quote: ... the resulting cell lysates were passed through a 20 G needle 100 times or heated using a Thermomixer (Eppendorf) at 55°C for 10 minutes with 1000 rpm agitation prior to the conventional RNA purification procedure in accordance with the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
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bioRxiv - Microbiology 2020Quote: Cells were concentrated from 10 ml of the enrichment cultures by centrifugation (4500×g, 20 min, 28°C) using a swing-bucket rotor (Eppendorf) and resuspended in approximately 200 µl of the supernatant ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Microbiology 2020Quote: ... 190 μl of the lysate was combined with 10 μl of 20 mg/ml proteinase K in a PCR plate and incubated in a MasterCycler ProS (Eppendorf) for 60 min at 60°C for digestion ...
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bioRxiv - Immunology 2022Quote: ... were exposed to transient thermal stress by incubating at a range of temperatures from 20 °C to 80 °C in a thermal cycler (Eppendorf) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... The 20 mL cultures used for all other analyses were incubated at 37°C in an Innova 40R shaking incubator (Eppendorf) at 230 rpm until they reached an OD600 of 0.7.
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bioRxiv - Genetics 2022Quote: ... The samples were then incubated at room temperature (about 25°C) at 450 rpm for 20 mins on Thermomixer (Eppendorf). Putting the samples on magnetic stand to facilitate removing supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... 100 U/ml DNase I in HBSS−/−) at 37°C for 20 minutes with constant agitation (300 RPM) in a ThermoMixer (Eppendorf). All subsequent steps were performed as previously described9,14 ...
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bioRxiv - Biochemistry 2020Quote: ... chloroform (4:2:1, v: v: v, containing 20 mM ammonium formate. The solutions were then placed into the wells of an Eppendorf twin-tec 96-well PCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Immunology 2020Quote: ... Peptides were eluted in 20 μL of 80% acetonitrile in 0.1% TFA and were concentrated to 4 μL (Concentrator 5301, Eppendorf AG). The peptides sample was then diluted to 5 μL by 0.1% TFA for LC-MS/MS analysis.
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bioRxiv - Molecular Biology 2022Quote: The 2 types of ascospores were cleaned by 2 washes with 10% and 20% sucrose solutions and 10-min centrifugation at 1,000 rpm (desktop centrifuge, Eppendorf, Germany); the supernatant was discarded after each centrifugation ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 0.1% Tween 20 in water) and centrifuged for 5 min at 500 g in a swinging bucket rotor centrifuge (5920R, Eppendorf). Nuclei pellets were resuspended in 1X Tagmentation Buffer (10 mM Tris pH 7.4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μL of paraffin oil was added in each reaction well with an automatic multichannel pipette (Eppendorf Xplorer, ref 4861000120).
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bioRxiv - Neuroscience 2023Quote: ... To make 10 μl of 20 μM primer pairs per each gene in V-shaped 96 well plate (Eppendorf, #951020401), 4 μl of forward and reverse primers were added to 2 μl H2O.
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bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...
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bioRxiv - Molecular Biology 2023Quote: The fully and semi-ejected ascospores were cleaned separately by 2 washes with 10% and 20% sucrose solutions and 10-min centrifugation at 1,000 rpm (desktop centrifuge, Eppendorf, Germany); the supernatant was discarded after each centrifugation ...
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bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Cancer Biology 2023Quote: 20-30 mg sections of snap frozen zebrafish liver tissue were transferred to pre-chilled Safe-Lock tubes (Eppendorf, 022363352) containing a cold 5/16 in ...
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bioRxiv - Neuroscience 2024Quote: ... samples were layered on top of the 105 µl cushion and spun at 10,000 g for 20 min at 4°C in a swing out rotor (A-8-11 swing bucket rotor, Eppendorf) to isolate CpxII bound to trans SNARE complexes (SNAREpins ...
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bioRxiv - Cell Biology 2024Quote: ... 40% glycerol) and boiled at 100°C on a heat block for 20 minutes and then centrifuged at 16,000g (Eppendorf 5415) for 3 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The cell suspension was centrifuged at full speed (7,197 × g) at 20 °C for 10 minutes in a refrigerated centrifuge (cat. 5430 R, Eppendorf, Hauppauge, NY). The supernatant was discarded ...
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bioRxiv - Systems Biology 2020Quote: ... then PBS-RI was added to bring the final concentration of Tween-20 to 0.01% (i.e. add 2508 μL to 836 μL sample, splitting the samples into multiple Eppendorf tubes for centrifugation). Cells were then centrifuged at 10,000xg for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The cultures were inoculated at an initial OD660nm of 0.1 and incubated under an air atmosphere and shaken at 200 rpm and at 20 °C in a New Brunswick™ Innova44 incubator (Eppendorf Nederland B.V ...
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bioRxiv - Microbiology 2021Quote: ... The cells were incubated with the dye and shaken for 20 minutes at room temperature in the dark, then washed by centrifugation (3 min, 8000 RPM, 28 °C (Eppendorf 5430R)) and resuspension in M2G three times ...
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bioRxiv - Synthetic Biology 2021Quote: ... and incubated at 30 °C for 20 hours (lid temperature 37 °C) in an Mastercycler pro384 vapo.protect thermocycler (Eppendorf, Hamberg, Germany). Scaled-up (15 μL ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Biochemistry 2019Quote: ... for 25 min at room temperature followed by centrifugation at 13 000 rpm for 10 min at 20 oC (Centrifuge 5415R; Eppendorf, Germany). Then the supernatant was dried in SpeedVac at 45 oC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were dislodged from a T75 flask and pelleted via centrifugation at 1300 rpm for 5 minutes at 20 °C (Eppendorf 5804R). The supernatant was aspirated ...
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bioRxiv - Microbiology 2022Quote: ... Twenty μL of supernatants were used as input controls and stored at −20°C in low-bind tubes (Eppendorf, Enfield, CT). For each sample ...
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bioRxiv - Neuroscience 2022Quote: ... 3 times with 10” ON and 30” OFF intervals on 20% power on ice and cleared by centrifugation at 21,000 rpm for 20min at 4°C (Eppendorf 5424R Centrifuge). 50µg of supernatant was mixed with NuPAGE LDS Sample Buffer (Thermo Scientific ...