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Citations for Eppendorf :
1 - 50 of 1101 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... then 60 °C for 2 hours while shaking at 1400 RPM (Eppendorf, ThermoMix C). To select for ECM proteins ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Microbiology 2020Quote: ... including human and microbial cells (4°C, Eppendorf 5810R centrifuge, 15 min, 3,220 rcf) represented in the “cell pellet” ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 37°C with 220 rpm shaking (Eppendorf, Thermo Mixer C). AcTEV protease AL-PHA assays ...
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bioRxiv - Synthetic Biology 2020Quote: ... These reactions were incubated for 2 h at 30°C with 500 rpm shaking (Eppendorf, Thermo Mixer C). MMP14 AL-PHA assays ...
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bioRxiv - Cancer Biology 2023Quote: ... and then gently shaken at 300 RPM for 2 hr at 37°C using a Thermomixer C (Eppendorf). Sample pH was then adjusted to 4 with 15 μL of 20% formic acid before loading onto conditioned SPE Bond-Elute pH columns (Agilent ...
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bioRxiv - Evolutionary Biology 2019Quote: ... pH 8.5 with 1 µg endoproteinase Lys-C (Wako) for 2 hours with shaking at 600 rpm at 37 °C in a thermomixer (Eppendorf). One µg of trypsin (Pierce ...
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bioRxiv - Biochemistry 2022Quote: ... Annealing was carried out by heating the mixture to 95 °C for 2 minutes followed by gradual cooling to 25 °C over 45 minutes in a thermocycler (Eppendorf).
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated at 37°C at 600 rpm with interval mixing (30 seconds mix, 2 minutes pause) on a Thermomixer C (2231000667, Eppendorf). After the last digestion ...
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bioRxiv - Biochemistry 2024Quote: ... #33045-U) and incubated for 2 h at 60 °C at 400 rpm on a thermomixer (Eppendorf ThermoMixer C, Germany, #EP5382000023). Then ...
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bioRxiv - Microbiology 2020Quote: ... (vi) 98°C for 2 min in a thermo-block (Eppendorf, Hamburg, Germany). Sterile DMEM treated in the similar methods served as negative controls ...
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bioRxiv - Cell Biology 2020Quote: ... The chondrocytes were incubated with the dye at 37°C for 2 hours at 300 rpm in a ThermoMixer C (Eppendorf, Hamburg, Germany). After incubation ...
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bioRxiv - Biochemistry 2022Quote: ... and incubated with Methoxamine hydrochloride (MeOX-HCl, 2%, 40 μl) at 60 °C for 2 hours at 400 rpm in a thermomixer (Eppendorf, USA). After adding N-methyl-N-(trimethylsilyl ...
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bioRxiv - Microbiology 2022Quote: ... followed by proteolytic digestion for 2 hours at 47 °C using a thermomixer (Eppendorf). Peptides were eluted with 50 mM TEAB pH 8.0 and centrifugation at 3,000 xg for 1 minute ...
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bioRxiv - Microbiology 2022Quote: ... followed by proteolytic digestion for 2 hours at 47 °C using a thermomixer (Eppendorf). Peptides were eluted with 50 mM TEAB pH 8.0 and centrifugation at 1,000 xg for 1 minute ...
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bioRxiv - Cell Biology 2022Quote: ... followed by proteolytic digestion for 2 hours at 47 °C using a thermomixer (Eppendorf). Peptides were eluted with 50 mM TEAB pH 8.0 and centrifugation at 1,000 xg for 1 minute ...
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bioRxiv - Biochemistry 2022Quote: ... followed by proteolytic digestion for 2 h at 47 °C using a thermomixer (Eppendorf). Peptides were eluted with 50 mM TEAB pH 8.0 and centrifugation at 1,000 × g for 1 min ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation for 2 hours at 47°C using an unmoving thermomixer (Eppendorf). Peptides were eluted with 40 µl TEAB (50 mM ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μM Al.488-labeled TDP-43-MBP-His6 was set up in low binding tubes (Eppendorf) in aggregation buffer and incubated with or without 100 μg/ml His6-TEV protease ...
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bioRxiv - Biochemistry 2022Quote: ... and RNA digestion carried out for 2 h at 52 °C on a ThermoMixer (Eppendorf). After cooling on ice ...
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bioRxiv - Cell Biology 2019Quote: ... The trypsin digestion proceeded for 2 hours at 37 °C on Thermomixer (750 rpm; Eppendorf). Subsequently ...
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bioRxiv - Cell Biology 2019Quote: ... The trypsin digestion proceeded for 2 hours at 37°C on Thermomixer (750 rpm; Eppendorf). Digested peptides were extracted from gels using 50% ACN solution with 2.5% formic acid (FA ...
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bioRxiv - Microbiology 2022Quote: Cells (2 ml cultures) were spun down (30 s Eppendorf centrifuge, 14,000 rpm, 4 °C), resuspended in 0.4 ml ice-cold growth medium and added to a screw cap Eppendorf tube containing 1.5 g glass beads (0.1 mm) ...
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bioRxiv - Cell Biology 2023Quote: ... and heated up to 37°C for 2 h while shaking (800 rpm, Thermomixer F1.5, Eppendorf). Following this ...
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bioRxiv - Cell Biology 2023Quote: ... The digestion was performed for 2 h at 40 °C on a Thermomixer (750 rpm; Eppendorf). Tryptic peptides were extracted info LC-MS vials by 2.5% formic acid (FA ...
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bioRxiv - Biophysics 2020Quote: ... with three ssDNA overhang strands on a bottom partially complementary to the sequence on the magnetic beads (mag2, Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Developmental Biology 2019Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Genomics 2019Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Genomics 2021Quote: ... the tube was incubated at room temperature for 2 minutes on a thermomixer (Eppendorf Thermomixer C, #5382000023) set to 1250 rpm ...
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bioRxiv - Physiology 2021Quote: ... These were then incubated at 70°C at 1400 rpm for 2 hours on a heat shaker (Eppendorf) and spun at 2500 x g for 5 min ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were thawed shortly before mass spectrometric analysis and shaken for 2 minutes at 2000rpm (thermomixer C, Eppendorf). Peptide concentrations were measured optically at 280nm (Nanodrop 2000 ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was hydrolyzed in 3N HCl for 2 hours at 95 °C on a shaking Thermomixer (Eppendorf). The reaction was quenched with 100 % methanol containing 40 μM L-norvaline (as an internal control) ...
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bioRxiv - Molecular Biology 2024Quote: A total of 350,000 HEK293-T cells were seeded in each well of 6-well plates (#EP0030720113, Eppendorf). Transfection was performed the day after at 40-50% cell confluence ...
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bioRxiv - Genomics 2020Quote: ... cultures were diluted to OD ∼ 0.02 in 1.4 mL of LB and were further grown for 2 h at 37°C on a Thermomixer (Eppendorf) with shaking (700 rpm) ...
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bioRxiv - Immunology 2022Quote: ... incubated with each antigen at certain concentration in PBS/2% FCS and transfer to a 37°C thermomixer (Eppendorf). Cells were collected after each period of incubation and fixed in IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Cell Biology 2020Quote: ... The homogenate was further incubated on ice for 45 min before centrifugation at 21,000 g and 4°C for 2 x 10 min in an Eppendorf 5424 R benchtop centrifuge (Eppendorf). Protein content of the lysate was determined by BCA assay (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was incubated at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany) to reduce viscosity ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were started by addition of 59.0 μM ubiquitin and 2 mM ATP then incubated at 37°C in a Mastercycler nexus (Eppendorf, Germany) thermocycler for 2 hrs unless otherwise stated ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... The obtained pellets were air-dried to rid them of residual acetone and solubilized with 120 μl Tris-SDS (10 mM Tris-HCl pH 7.5 and 2% SDS) at 56° C for 10 min and shaking at 1000 rpm in a table-top thermomixer (Eppendorf). The resulting suspension was centrifuged 20,000×g for 10 minutes at room temperature in a table-top micro-centrifuge (Eppendorf) ...
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bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were grown in the rich medium YEP + 2% glucose (YEPD) at 25°C with a rotation of 2000 rpm (New Brunswic Innova 43R, Eppendorf). To arrest cells in late G1 by overexpressing non-degradable Sic1(9m ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred in a new tube and the cytosolic fraction was dried under vacuum for 2 h at 55°C in a Speedvac (Eppendorf). Pellets were then stored at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... 6 mL of the selection culture in 2 mL centrifuge tubes was pelleted at 5000 rpm for 5 minutes at 4°C in a microcentrifuge (Eppendorf, 5242R). The supernatant was removed except for the last ∼200 µL ...