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Citations for Eppendorf :
251 - 296 of 296 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Microbiology 2022Quote: ... the digested protein fraction was forced through the filter and collected in a 500 μl LoBind plate (Eppendorf, Hamburg, Germany). The filter was further washed with 100 μl 50 mM ABC followed by 100 μl H2O ...
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bioRxiv - Genomics 2022Quote: ... and the pellet was resuspended in 500 ul Buffer CT and the mixture was transferred to a 2.0 mL Protein LoBind tube (Eppendorf # 022431102). The suspension was spun again at 16,000 x g at 4 C for 2 min and the supernatant discarded ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (∼200 ul) was transferred to a 70uM filter (Fisher # NC1444112) set in a new 1.5 mL Protein LoBind tube (Eppendorf # 022431081). The tube with the 70 uM filter was spun on a mini-centrifuge (Ohaus # FC5306 ...
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bioRxiv - Immunology 2020Quote: ... plates were placed on magnetic plates and digestion reaction containing trypsinized peptides was transferred to a protein LoBind Eppendorf tube (Eppendorf, Cat ...
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bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of 200 µM monomeric αSyn was incubated in fibrillation buffer (Supplementary Table S2) in a 1.5 mL Protein LoBind Eppendorf tube (Eppendorf, Germany) on an orbital shaker (PCMT Thermoshaker Grant-bio ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were pelleted (600 x g, 2 min) and the supernatant was transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 2 × 300 μL HPLC grade water and the washes combined with the original supernatant ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were fixed in 2% paraformaldehyde for 55 minutes at room temperature in 2 mL Protein LoBind tubes (Eppendorf 022431064). Fixative was removed and tissues were washed 4x 10 minutes with 1.5 mL PBS with 0.5% Triton X-100 (PBT) ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Protein concentration in the supernatant was determined according to the Bradford Ultra method (Bradford, 1976) on a Bio-Spectrophotometer (Eppendorf) at 595 nm ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
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bioRxiv - Microbiology 2023Quote: ... The beads were pelleted (600 x g, 3 min) and the supernatant transferred to a fresh low protein binding tube (Eppendorf). The beads were washed with 1 x 200 µL HPLC-grade water and the washes combined with the original supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... The flow rate was 0.2 ml/min and 48 × 100 μl fractions were collected in a low-protein binding 96-deep-well plate (Eppendorf) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... though the maximum volume of beads + gDNA + binding buffer should not exceed 1.4 ml for a single 1.5 ml Protein LoBind tube (Eppendorf, Cat. 30108442). Multiple tubes can be used and pooled at the restriction enzyme digest step ...
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bioRxiv - Bioengineering 2024Quote: ... the scaffolds were immersed in 1.5 mL BMP-2 (100 µg/mL) diluted in PBS in a low protein-binding falcon tube (Eppendorf, UK). The bottom of the tube was gently tapped on a surface to remove any bubbles between the pores of the scaffold to allow the solution to completely contact the scaffold ...
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bioRxiv - Biochemistry 2020Quote: ... The protein solution was then kept on ice whilst the absorbance at 280nm was measured using a BioPhotometer® D30 (Eppendorf) spectrophotometer ...
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bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
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bioRxiv - Genomics 2022Quote: ... they froze and crushed a single, adult female spider with a pellet pestle (Fisher, #12-141-364) in a Protein LoBind tube (Eppendorf, #022431081) containing 200 uL of Buffer CT ...
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bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
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bioRxiv - Neuroscience 2021Quote: ... CSF samples were collected by pre-pulled glass capillary tube from the cisterna magna and then transferred to 0.5mL Protein LoBind Eppendorf tubes (Eppendorf Cat #022431064) for centrifugation at 12,700 rpm for 7 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Biophysics 2021Quote: ... Experiments on the seconds to minutes time scale were performed at room temperature in 96-well Protein LoBind plates (Eppendorf, Hamburg, Germany).
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bioRxiv - Genomics 2020Quote: ... 3X in Ribohyb buffer and resuspended in a final volume of 10 ul Ribohyb buffer in a non-stick tube (Eppendorf Protein LoBind). The 10 µl hybridization reactions were added to 10 µl of resuspended beads for a binding reaction volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
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bioRxiv - Neuroscience 2020Quote: Aggregate enrichment was performed by centrifugation of a total of 150 μg protein at 20’800 g in a tabletop centrifuge (Eppendorf 5417r, Hamburg, Germany) for 1 hour at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... each SCN was cut into 3 pieces with a scalpel on a glass slide and transferred to a protein LoBind tube (Eppendorf, Hamburg, Germany) prefilled with 250 µL lysis buffer (100 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
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bioRxiv - Genomics 2023Quote: ... approximately 1.0-2.0 x 105 GFP+ neurons per pup were collected by FACS into a 1.5 mL Protein LoBind Tube (Eppendorf, cat. no. #Z666505).
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
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bioRxiv - Biochemistry 2021Quote: ... The molecular weight cut-off columns were rinsed by addition of equal volumes of Milli-Q water and the proteins were dried with a speed-vac (Eppendorf, Vacufuge Concentrator, Stevenage, UK).
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bioRxiv - Biochemistry 2022Quote: ... the supernatant was removed and cells were resuspended in cold PBS at 20 million cells/mL and then distributed into 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind tubes (Z666505-100EA) at 1 mL each ...
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bioRxiv - Biochemistry 2023Quote: ... Polypropylene standard safe-lock microcentrifuge vials and corresponding low protein binding vials of 1.5 mL size from three manufacturers were used: Manufacturer A (Eppendorf Safe-Lock Tubes no. 0030120086 and Protein LoBind® Tubes no. 0030108116, Eppendorf SE, Hamburg, Germany), Manufacturer B (Pierce™ Microcentrifuge Tubes no ...
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bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...