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Citations for Eppendorf :
51 - 100 of 131 citations for L Cysteine S Methyl S Methyl D3 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was then centrifuged at 1000 rpm for 30 s (5804/ 5804 R – Benchtop Centrifuge, Eppendorf North America, CT, USA), mixed on a plate shaker (Siemens DPC MicroMix 5 ...
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bioRxiv - Microbiology 2024Quote: ... The traps were washed three times with 150 µL S-Trap buffer and placed in clean 1.5 mL microcentrifuge tubes (Eppendorf Protein LoBind). Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade ...
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bioRxiv - Evolutionary Biology 2019Quote: ... pre-loaded with 70µl/well of bacterized CM and incubated at 43°C for 1.5 min in a thermocycler (Eppendorf Mastercycler® pro S). Cells were randomly assigned to plate wells for both control (no heat shock ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Cell Biology 2022Quote: ... the gel was then flushed twice with 100 µL of warm medium at 187.5 µL/s flow (Eppendorf Xplorer automatic pipet; Eppendorf). This generated a 3D-ECM layer on the membrane with a thickness of 20-85 µm ...
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bioRxiv - Neuroscience 2023Quote: ... S-opsin-positive cells (S-cones) and negative cells with cone morphology (M-cones) were separately collected using a micromanipulator (Eppendorf TransferMan 4r) and transferred into 8 µL lysis buffer (Clontech SMARTer Ultra Low Input RNA Kit for Sequencing - v3) ...
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bioRxiv - Developmental Biology 2019Quote: ... The embryos were deyolked in ice cold ringer’s solution using micropipette tip and spun at 100g for 2 minutes at cooled table top centrifuge at 40C (Eppendorf® centrifuge 5418R). The supernatant was discarded and the embryo bodies were trypsinized using TrypLE Express (Thermoscientific® ...
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bioRxiv - Microbiology 2020Quote: ... (vi) 98°C for 2 min in a thermo-block (Eppendorf, Hamburg, Germany). Sterile DMEM treated in the similar methods served as negative controls ...
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bioRxiv - Genetics 2021Quote: ... 72°C 45 s and a final extension at 72°C for 5 min was run on an Eppendorf Mastercycler® (Eppendorf AG, Hamburg Germany). Amplicons were observed on a 1.5% agarose gel (Bioline (Aust ...
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bioRxiv - Microbiology 2019Quote: ... four 50 mL aliquots from each trap were centrifuged at 3214 x g and 4°C for 30 minutes (Eppendorf 5810 R, S-4-104 rotor). The supernatant was decanted after centrifugation and cell pellets were stored at −80°C until ready for freeze-drying and lipid extraction ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
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bioRxiv - Bioengineering 2023Quote: ... coli JM108 were grown in a 1.8 L (1.0 L net volume, 0.5 L batch volume) computercontrolled bioreactor (DASGIP parallel bioreactor system, Eppendorf AG, Germany). The bioreactor was equipped with a pH probe and an optical dissolved oxygen probe (Hamilton Bonaduz AG ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µl of the bacterial samples were then kept at 98°C for 15 min to undergo lysis in a static thermomixer (Eppendorf GmbH, Germany) according to the reported protocol (Škulj et al. ...
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bioRxiv - Bioengineering 2020Quote: ... nitric acid and incubating at 98°C for 2 h with shaking at 300 rpm in an Eppendorf® ThermoMixer® Comfort 5355 dry block shaker (Eppendorf UK Ltd, Stevenage, Herts, UK).
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Immunology 2022Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼ 0.8 ...
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bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Microbiology 2023Quote: Bioreactor cultivations were performed using 1.0 L DASGIP reactors (Eppendorf). Fermentations were performed using YNB with 10 g L−1 (NH4)2SO4 as the nitrogen source ...
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bioRxiv - Synthetic Biology 2020Quote: Bioreactor cultivations were performed in 1.3-L fermenters (BioFlow120, Eppendorf, Germany) with a working volume of 0.5 L mineral salt medium (0.4 L mineral salt medium in fed-batch) ...
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bioRxiv - Synthetic Biology 2020Quote: Batch fermentations were conducted in 1 L DASGIP bioreactors (Eppendorf, Germany) with an initial volume of 550 mL ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was scaled-up in a 1.3 L bioreactor (Eppendorf, USA) with 0.5 L initial volume ...
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bioRxiv - Microbiology 2022Quote: ... viennensis was grown as a continuous culture in 2 L bioreactors (Eppendorf) filled with 1.5 L of fresh water medium (FWM ...
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bioRxiv - Synthetic Biology 2019Quote: The bioreactor cultivations were carried out in 1.4 L DASGIP reactors (Eppendorf, Germany). Cultivation temperature was controlled at 28 °C ...
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bioRxiv - Synthetic Biology 2023Quote: Fed-batch fermentations were conducted using a 6.6 L bioreactor (BioFlo 320, Eppendorf) containing 1.7 L R/2 medium (pH 6.8 ...
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bioRxiv - Synthetic Biology 2020Quote: ... We then set up a BioFlo120 system with a 5 L bioreactor (Eppendorf, B120110002) and added 3 L of SC-His medium supplemented with 15% glucose after autoclaving ...
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bioRxiv - Systems Biology 2023Quote: ... Cultivations were carried out in DasGip 1-L stirrer-pro vessels (Eppendorf, Jülich, Germany). The working volume was 500 mL ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were seeded on poly-L-lysine pretreated (0.001%, 1h) 24-well imaging plates (Eppendorf, Germany) at a density of 1e05 cells/well ...
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bioRxiv - Plant Biology 2022Quote: ... Fermentation was conducted in approximately 3 L of culture in a bioreactor BioFlo120 (Eppendorf, Hamburg, Germany) at 30°C for 5 days ...
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bioRxiv - Microbiology 2023Quote: ... intermedia CBS 141442 was grown in controlled stirred 1-L bioreactor vessels (DASGIP, Eppendorf, Hamburg, Germany) containing 500 mL synthetic defined minimal Verduyn media with 2% Glucose ...
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bioRxiv - Bioengineering 2023Quote: Chemostat cultivations were performed at 28°C in DasGip systems with 1 L stirrer -pro vessels (Eppendorf) as previously reported63 ...
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bioRxiv - Immunology 2020Quote: ... and fermentation was conducted in a CelliGen 310 Bioreactor with a 7.5 L vessel (Eppendorf, New York, USA), controlled by the Eppendorf Bio Command software ...
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bioRxiv - Biochemistry 2022Quote: ... 15 L of T-20052 (53) medium was prepared in a 20-liter Bioflow IV bioreactor (Eppendorf/NBS). The seed culture was collected and centrifuged at 3,000 x g for 10 minutes at 25¼;C ...
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bioRxiv - Bioengineering 2023Quote: Bioreactor fermentations at bench-top level were performed in DasGip 1-L stirrer-pro vessels (Eppendorf, Jülich, Germany) at 30 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Synthetic Biology 2021Quote: ... 40-120 μl of culture supernatant was dried in 1.5 ml microcentrifuge tubes with a rotational vacuum concentrator (Concentrator Plus, Eppendorf) and derivatised in 20 μl methoxyamine chloride (30 mg/ml in pyridine ...
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bioRxiv - Plant Biology 2021Quote: Seeds of tobacco (Nicotiana tabacum L. cv. SR1) or Arabidopsis Colombia were placed in a 1.7 mL micro-tube (Eppendorf) filled with sodium hypochlorite (0.5% active material ...
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bioRxiv - Neuroscience 2020Quote: ... washed twice with 160 µL PBS and nearly 40,000 cells per well transferred onto the membrane and dried with a plate thermomixer (Eppendorf) at 50 °C ...
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bioRxiv - Immunology 2020Quote: ... Process development was performed in a scale-down models in a 10 L glass-vessel bioreactor (BioFlo 320, Eppendorf). Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf) ...
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bioRxiv - Immunology 2020Quote: ... Established process parameters were then scaled up to 120 L in a BioFlo Pro 150 stainless steel fermenter (Eppendorf). Phosphate depletion during the growth phase induced UF6b expression into inclusion bodies ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were eluted in 40μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5μl by 0.1% TFA.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted with freshly prepared 60 μl of 1.25 % v/v ammonium hydroxide (NH4OH)/80 % v/v ACN and dried using a SpeedVac centrifuge (Eppendorf). Dried peptides were resuspended in 6 μL buffer A (2 % v/v ACN/0.1 % v/v TFA) ...
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bioRxiv - Molecular Biology 2023Quote: ... 66 Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). They were then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA.
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Samples were then prepared for LC-MS/MS analysis by diluting them to 5 μl with 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 5 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...