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Citations for Eppendorf :
1 - 50 of 390 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... (vi) 98°C for 2 min in a thermo-block (Eppendorf, Hamburg, Germany). Sterile DMEM treated in the similar methods served as negative controls ...
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bioRxiv - Microbiology 2020Quote: ... 10 g NaCl) in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf) were used to express the I53-50A or I53-50B.4PT1 proteins grown ...
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bioRxiv - Biochemistry 2022Quote: ... 15 L of T-20052 (53) medium was prepared in a 20-liter Bioflow IV bioreactor (Eppendorf/NBS). The seed culture was collected and centrifuged at 3,000 x g for 10 minutes at 25¼;C ...
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bioRxiv - Immunology 2022Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼ 0.8 ...
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bioRxiv - Immunology 2020Quote: ... 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8 ...
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bioRxiv - Microbiology 2022Quote: ... viennensis was grown as a continuous culture in 2 L bioreactors (Eppendorf) filled with 1.5 L of fresh water medium (FWM ...
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bioRxiv - Cancer Biology 2023Quote: SK-N-BE(2) cells (6000-8000 cells/well) were seeded in 24-well plates (Eppendorf) overnight and prior to treatment with retinoic acid (RA)(10 μM ...
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bioRxiv - Physiology 2023Quote: ... for EVs isolation on cell culture flask T-75 (1.5 − 2 × 106/ per flask; Eppendorf, Hamburg, Germany) and for immunostaining on Cell Imaging Cover Glasses (0.1 × 106 / per chamber ...
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bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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bioRxiv - Physiology 2024Quote: ... N = number of sample replicates (groups of n=∼20 tardigrades in a single Eppendorf tube). The majority of samples at -10°C remained unfrozen ...
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bioRxiv - Cell Biology 2022Quote: ... bound proteins were eluted from the streptavidin beads with 50 µl of Laemmli-DTT sample buffer containing 500 nM D-Biotin at 98°C by shaking at 1,000 rpm in a ThermoMixer (Eppendorf™). For mass spectrometry analysis ...
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bioRxiv - Bioengineering 2023Quote: ... coli JM108 were grown in a 1.8 L (1.0 L net volume, 0.5 L batch volume) computercontrolled bioreactor (DASGIP parallel bioreactor system, Eppendorf AG, Germany). The bioreactor was equipped with a pH probe and an optical dissolved oxygen probe (Hamilton Bonaduz AG ...
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bioRxiv - Cell Biology 2020Quote: ... The whole lane was excised from the gel and future cut into approximate 3 x 1 x 2 mm3 slices (L x W x H) and each slice were placed into a separate LoBind tubes (Eppendorf) for destaining ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 2 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Bioengineering 2024Quote: ... 500 µl of the bacterial samples were then kept at 98°C for 15 min to undergo lysis in a static thermomixer (Eppendorf GmbH, Germany) according to the reported protocol (Škulj et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples of N-NTD and N-NTD-SR at 20 µM were prepared in low-binding microtubes (Eppendorf® LoBind) containing 20 mM Tris-HCl buffer (pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... nitric acid and incubating at 98°C for 2 h with shaking at 300 rpm in an Eppendorf® ThermoMixer® Comfort 5355 dry block shaker (Eppendorf UK Ltd, Stevenage, Herts, UK).
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bioRxiv - Biophysics 2022Quote: ... Cells were plated in a T-25 cell culture flask (Eppendorf, 0030710126) coated with 0.1% gelatin solution (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Dissociated GBM cells were cultured in cell-culture flasks (from Eppendorf, T-75) filled with Neurobasal A (NBA ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Microbiology 2021Quote: ... The n-heptane layer was spectrophotometrically scanned between 230 and 300 nm (BioSpectrometer, Eppendorf). The presence of ergosterol (As281.5 peak ...
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bioRxiv - Microbiology 2023Quote: Bioreactor cultivations were performed using 1.0 L DASGIP reactors (Eppendorf). Fermentations were performed using YNB with 10 g L−1 (NH4)2SO4 as the nitrogen source ...
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bioRxiv - Synthetic Biology 2020Quote: Bioreactor cultivations were performed in 1.3-L fermenters (BioFlow120, Eppendorf, Germany) with a working volume of 0.5 L mineral salt medium (0.4 L mineral salt medium in fed-batch) ...
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bioRxiv - Synthetic Biology 2020Quote: Batch fermentations were conducted in 1 L DASGIP bioreactors (Eppendorf, Germany) with an initial volume of 550 mL ...
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bioRxiv - Molecular Biology 2021Quote: Protein expression was scaled-up in a 1.3 L bioreactor (Eppendorf, USA) with 0.5 L initial volume ...
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bioRxiv - Molecular Biology 2024Quote: A total of 350,000 HEK293-T cells were seeded in each well of 6-well plates (#EP0030720113, Eppendorf). Transfection was performed the day after at 40-50% cell confluence ...
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bioRxiv - Synthetic Biology 2019Quote: The bioreactor cultivations were carried out in 1.4 L DASGIP reactors (Eppendorf, Germany). Cultivation temperature was controlled at 28 °C ...
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bioRxiv - Synthetic Biology 2023Quote: Fed-batch fermentations were conducted using a 6.6 L bioreactor (BioFlo 320, Eppendorf) containing 1.7 L R/2 medium (pH 6.8 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and InjectMan NI 2 (Eppendorf). Cas9 protein (500 ng/μL) ...
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bioRxiv - Molecular Biology 2019Quote: ... The beads were then resuspended in 100 µl PBS-T and transferred to a fresh 1.5 ml DNA LoBind Tube (Eppendorf #0030108051). After removal of the PBS-T ...
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bioRxiv - Biochemistry 2019Quote: ... Varying volumes of viral supernatant were mixed with 5 × 106 to 1 × 107 log phase Jurkat cells in a final volume of 10 ml within a T-25 flask (Eppendorf) and subsequently cultured for 72 hours ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... swabs were introduced into a DNA/DNase/RNase free 1.5 ml Eppendorf Biopur tube (Cat. N° 0030 121.589, Eppendorf, Germany) containing 500 μl of nuclease free water (Cat ...
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bioRxiv - Synthetic Biology 2020Quote: ... We then set up a BioFlo120 system with a 5 L bioreactor (Eppendorf, B120110002) and added 3 L of SC-His medium supplemented with 15% glucose after autoclaving ...
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bioRxiv - Systems Biology 2023Quote: ... Cultivations were carried out in DasGip 1-L stirrer-pro vessels (Eppendorf, Jülich, Germany). The working volume was 500 mL ...
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bioRxiv - Molecular Biology 2019Quote: ... using a Realplex 2 thermocycler (Eppendorf). The PCR conditions were 95°C for 3 min ...
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bioRxiv - Immunology 2022Quote: ... The micromanipulator (Eppendorf InjectMan NI 2) was mounted on an Andor Spinning Disc Microscope and microinjection was performed using the microinjector FemtoJet with a 100X oil immersion objective to facilitate immediate visualization and image acquisition after microinjection ...
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bioRxiv - Cell Biology 2023Quote: ... The micromanipulator (Eppendorf InjectMan NI 2) was positioned and microinjection was performed using microinjector (Eppendorf FemtoJet ...
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bioRxiv - Cancer Biology 2024Quote: ... on a RealPlex 2 Thermocycler (Eppendorf).
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bioRxiv - Microbiology 2024Quote: ... and Mastercycler ep Realplex 2 (Eppendorf). Data was normalized by GAPDH or sigA expression level and all primers were designed using GenScript primer design software ...
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bioRxiv - Immunology 2021Quote: Xela DS2 (passages 120 – 130) and Xela VS2 (passages 125 – 135) (n = 4 independent trials) were seeded in a 6-well plate (Eppendorf) at a cell density of 625,000 cells/well in 1 mL of Xela complete media and allowed to adhere overnight at 26 °C ...
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bioRxiv - Immunology 2019Quote: ... 1*106 T cells/condition were harvested 72h after α-CD3/α-CD28 activation and transferred to DNA Lo-binding Eppendorf tubes (Eppendorf). Cells were washed once with PBS and supernatant was completely removed ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... using a RealPlex 2 thermocycler (Eppendorf, 2894). All primers were designed and purchased from Integrated DNA Technologies and their sequences are listed in Supplementary file 1.
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bioRxiv - Systems Biology 2021Quote: ... Cells were seeded on poly-L-lysine pretreated (0.001%, 1h) 24-well imaging plates (Eppendorf, Germany) at a density of 1e05 cells/well ...
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bioRxiv - Plant Biology 2022Quote: ... Fermentation was conducted in approximately 3 L of culture in a bioreactor BioFlo120 (Eppendorf, Hamburg, Germany) at 30°C for 5 days ...
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bioRxiv - Microbiology 2023Quote: ... intermedia CBS 141442 was grown in controlled stirred 1-L bioreactor vessels (DASGIP, Eppendorf, Hamburg, Germany) containing 500 mL synthetic defined minimal Verduyn media with 2% Glucose ...