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Citations for Eppendorf :
251 - 300 of 311 citations for KHK IN 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and snap-frozen by placing the tissue in pre-chilled low bind 2 mL microfuge tubes on dry ice (#022431048 Eppendorf). FC sections (male n = 27 ...
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bioRxiv - Bioengineering 2024Quote: ... the scaffolds were immersed in 1.5 mL BMP-2 (100 µg/mL) diluted in PBS in a low protein-binding falcon tube (Eppendorf, UK). The bottom of the tube was gently tapped on a surface to remove any bubbles between the pores of the scaffold to allow the solution to completely contact the scaffold ...
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bioRxiv - Microbiology 2023Quote: Overnight cultures of the investigated strains were prepared in 1 mL aliquots of TSB in 2 mL microcentrifuge tubes (Eppendorf) which were incubated horizontally with shaking (120 rpm ...
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bioRxiv - Developmental Biology 2024Quote: ... One nL morpholino (dissolved in 0.2 M KCl with 0.05% phenol red at 3 mg/mL) was microinjected into haploid embryos at the 1 or 2-cell stage using FemtoJet and InjectMan NI2 (Eppendorf).
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bioRxiv - Molecular Biology 2019Quote: ... 100 µl of sample was saved as input and the rest was split into 2 ml DNA LoBind tubes (Eppendorf, D1579630) and 10 ml of anti-Flag antibody was added (F1804 ...
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bioRxiv - Biophysics 2019Quote: ... 6 mL of the selection culture in 2 mL centrifuge tubes was pelleted at 5000 rpm for 5 minutes at 4°C in a microcentrifuge (Eppendorf, 5242R). The supernatant was removed except for the last ∼200 µL ...
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bioRxiv - Cancer Biology 2021Quote: Plasmids were injected into the ventricle of isoflurane-immobilized pups at postnatal day 2 using an Eppendorf Femtojet microinjector (Eppendorf, 5247000030), followed by electroporation (5 square pulses ...
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bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
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bioRxiv - Systems Biology 2021Quote: ... DW determination was performed by centrifuging 2 ml of the fresh sample placed in a pre-weighed Eppendorf tube in a MiniSpin centrifuge (Eppendorf, Spain) at maximum speed (13.200 rpm ...
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bioRxiv - Genetics 2022Quote: ... Yeast strains were manually inoculated into 400 µL of liquid SC -lys medium with G418 and grown overnight in 2 mL 96 well plates at 30.0 °C with 1000 rpm mixing using a MixMate (Eppendorf, Hamburg, Germany). The following morning ...
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bioRxiv - Microbiology 2022Quote: ... and 100 mg of freeze-dried tissue was transferred to 2-ml microcentrifuge tubes (Eppendorf Safe-Lock tubes; Eppendorf, Hamburg, Germany) containing a single 4-mm stainless-steel grinding ball (SPEX SamplePrep ...
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bioRxiv - Systems Biology 2021Quote: ... DW determination was performed by centrifuging 2 ml of the fresh sample placed in a pre-weighed Eppendorf tube in a MiniSpin centrifuge (Eppendorf, Spain) at maximum speed (13.200 rpm ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... was incubated with 700 μl hexafluoro-2-propanol (HFIP) overnight and divided into 108 μg doses in LoBind reaction tubes (Eppendorf AG). Samples were lyophilized in a rotational vacuum concentrator system connected to a cold trap (both Martin Christ Gefriertrocknungsanlagen GmbH) ...
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bioRxiv - Neuroscience 2021Quote: ... A plasmid DNA solution of 1.4 µg/uL was injected into embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C scope (Leica Microscopes ...
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bioRxiv - Immunology 2022Quote: ... bacterial stocks were grown at 30°C for 2 days to an OD600 of 0.4 approximately 6.3×106CFU/mL (Eppendorf, BioPhotometer; Hamburg, Germany). Bacteria were washed with sterile PBS and aspirated through a 27G needle against the side of the tube multiple times to break up bacterial clumps ...
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bioRxiv - Genomics 2023Quote: ... The nuclei were diluted to ∼300 nuclei per uL across 2 x twin.tec™ 96 Well LoBind PCR Plates (Eppendorf, 0030129512). The reverse transcription reaction was set up as in Cao et al. ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl of the sample was mixed with an equal volume of buffered phenol and 20 μl of 20% SDS in a 2 mL centrifuge tube (Eppendorf, Germany). After adding 0.5g of 2 mm zirconia beads (BioSpec Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted samples were aliquoted in 2 mL tubes and vaporized under vacuum at 40°C using SpeedVac (Eppendorf, Hamburg, Germany). The samples were resuspended and further diluted in sterile water before applying to MBA ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then exposed to phages at indicated MOIs or incubated untreated for 30 min in a 2 mL Eppendorf tube under shaking conditions (<650 rpm in an Eppendorf ThermoMixer). For TIRF microscopy ...
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bioRxiv - Bioengineering 2023Quote: ... Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf, Germany). Each prepared sample was separated on Hi-pH column (Thermo ...
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bioRxiv - Genetics 2024Quote: ... the samples were washed 3 times with 500 μl Perm wash (centrifugation for 2 minutes at 1600 rpm (Eppendorf centrifuge 5415R)) ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was filtered through a 50 µm filter (Sysmex; 04-004-2327) into a 2 mL microcentrifuge tube (Eppendorf; 022431048). An additional 0.5 mL of homogenization buffer was used to wash the Dounce homogenizer and filter ...
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bioRxiv - Biochemistry 2024Quote: ... #33045-U) and incubated for 2 h at 60 °C at 400 rpm on a thermomixer (Eppendorf ThermoMixer C, Germany, #EP5382000023). Then ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Cell Biology 2020Quote: ... The chondrocytes were incubated with the dye at 37°C for 2 hours at 300 rpm in a ThermoMixer C (Eppendorf, Hamburg, Germany). After incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the medium exchange was conducted under semi-sterile conditions directly at the microscope with a 10 μl microloader tip (Microloader Tip 0,5-10 μl / 2-20 μl, Eppendorf AG, Hamburg, Germany) every two days.
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bioRxiv - Biochemistry 2021Quote: ... Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf; EP0030700112-300EA) upon reaching high density ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
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bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
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bioRxiv - Biophysics 2022Quote: ... Ltd., Kanagawa, Japan) controlled by a pneumatic microinjector (IM-11-2, Narishige, Tokyo, Japan) and a micromanipulator (Transferman NK2, Eppendorf, Hamburg, Germany) and transferred into 4 ml RNase-free water (06442-95 ...
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bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
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bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
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bioRxiv - Neuroscience 2021Quote: ... a whole eye from wild type and mutant adult zebrafish or 100 zebrafish mutant larvae were transferred to 2 ml Eppendorf microcentrifuge tubes (Eppendorf, Hamburg, Germany). 200 μl of 2 M hydroxylamine (pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... were arranged on double-sided sticky tape using a paintbrush and microinjected at the posterior pole of each egg with wAlbB (2×108 bacteria/ml) using a FemtoJet microinjector system (Eppendorf, NSW, Australia). The microinjected eggs were then placed on tissue paper on the surface of artificial manure pats to hatch ...
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bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1800 µl of solution from both tubes (batch 1) were transferred to a 2 ml Eppendorf and vortexed (Eppendorf Thermomixer C) for 15 minutes at 2000 rpm (4 °C) ...
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bioRxiv - Genetics 2024Quote: ... placed in an injection arena (9 cm-diameter Petri dish containing a layer of 1% agarose) and injected with ∼60 ng of Pmtra-2 or EGFP dsRNA (negative control) using a FemtoJet microinjector (Eppendorf, Hamburg, Germany). Injectees were maintained in a two-ounce plastic cup with corn leaves until they became adults ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Bioengineering 2020Quote: ... Single colonies were grown overnight in 1 mL of SC or SC-ura medium supplemented with 2% glucose in individual wells of the 24-well microtiter plates under constant blue light in an orbital shaker (Eppendorf, New Brunswick, USA) at 30°C and at 200 rpm agitation ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were resuspended and 50 μl of each pre-culture was used to inoculate 1.5 mL of fresh YPD media (corresponding to an optical density of 0.5 at 600 nm) in 96-deep-well plates (LoBind®, 2 mL, cat no. 0030504305, Eppendorf AG, Hamburg, Germany). Plates were covered with an air permeable membrane and incubated while shaking at 300 rpm and 30 °C for 6 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... The embryos were deyolked in ice cold ringer’s solution using micropipette tip and spun at 100g for 2 minutes at cooled table top centrifuge at 40C (Eppendorf® centrifuge 5418R). The supernatant was discarded and the embryo bodies were trypsinized using TrypLE Express (Thermoscientific® ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
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bioRxiv - Microbiology 2023Quote: ... algal cells with the attached bacteria in the treatment with the species consortium were also collected by a pipette from the formaldehyde-fixed suspended sample and transferred into 2 ml safe-lock tubes (Eppendorf, Wesseling-Berzdorf, Germany). To avoid an excessive fixation ...
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bioRxiv - Developmental Biology 2024Quote: ... One- or 2-cell stage embryos were injected with approximately 20 nl of the injection mixture (manually or using an Eppendorf FemtoJet 4x microinjector) and maintained at 20°C until the 64-cell stage ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).