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Citations for Eppendorf :
651 - 700 of 849 citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Sorted nuclei were then centrifuged at 1000 g for 15 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3), and supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and Sample Reducing Agent (10x) and incubated for 5 min at 70°C and 1100 rpm using a ThermoMixer comfort (Eppendorf).
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... and streptomycin (10 µg ml−1) at 37° C for 40 min with shaking at 800 rpm in a thermomixer (Eppendorf). Digested medullae were resuspended and the enzymatic reaction quenched by addition of 10 volumes cold CMF-HBSS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... in 200 µl volumes grown at 37 °C with 200 rpm shaking in an Innova 42R Incubator (Eppendorf, United Kingdom) for 22 h growth periods (‘days’) ...
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bioRxiv - Systems Biology 2023Quote: ... Homogenized islets were filtered with 30 µm filter (CellTrics, Sysmex) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei were resuspended in 400 µL of sort buffer (1% fatty acid-free BSA ...
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bioRxiv - Systems Biology 2023Quote: ... were added without disturbing the pellet and nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed without disturbing the pellet and leaving ∼2-3 µl behind ...
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bioRxiv - Systems Biology 2023Quote: ... and incubated on ice for 1 min before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for thirty minutes with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... The kinase reaction was carried out at 30 °C for two hours with shaking (300 rpm) in a Thermomixer (Eppendorf). The reaction was stopped by addition of 5 μL of 4X SDS-PAGE loading buffer [250 mM Tris ...
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was diluted with RNAse free water to a concentration of 500ng/20ul and denatured at 70°C for 15min in a Mastercycler (Eppendorf). Mastermix was prepared by mixing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was performed in a final volume of 60 μL for 60 min at 30°C under stirring (750 RPM, Thermomixer, Eppendorf). γS-ATP was removed by dialyzing the reaction mix against kinase buffer ...
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bioRxiv - Biophysics 2023Quote: ... monomer exchanges were performed by incubating a mixture of both variants for 40 minutes at 43 °C and 300 rpm in a shaking incubator (Thermomixer 5436, Eppendorf). This method was also employed to obtain Cdc371-Hsp902-fusion heterodimers (one Cdc37 per Hsp90 dimer) ...
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bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Genetics 2023Quote: ... This mix was incubated for 30 minutes at 37°C to form ribonucleoprotein complexes and injected into hermaphrodite adult gonads using a Transjector 5246 (Eppendorf). F1 progeny were singled out from plates displaying a high proportion of the dumpy (dpy-10 ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at 37 °C for 4 hrs under gentle orbital agitation (300 rpm on Eppendorf Thermomixer) and centrifuged at 15 000 × g for 5 min prior to SEC analysis ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the samples were 10000xg centrifuged for 10 min in a cooled centrifuge (4°C; Centrifuge 5415 R, Eppendorf, Hamburg, Germany), and the supernatant transferred to new microcentrifuge tubes ...
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bioRxiv - Biochemistry 2022Quote: ... yeast cells were grown in the rich medium YEP + 2% glucose (YEPD) at 25°C with a rotation of 2000 rpm (New Brunswic Innova 43R, Eppendorf). To arrest cells in late G1 by overexpressing non-degradable Sic1(9m ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred in a new tube and the cytosolic fraction was dried under vacuum for 2 h at 55°C in a Speedvac (Eppendorf). Pellets were then stored at 4°C ...
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bioRxiv - Pathology 2023Quote: ... The samples were extracted overnight at 4℃ and then centrifuged at 4,000 rpm for 5 min at 4°C using a 5810 R fixed-angle rotor centrifuge (Eppendorf, Germany). 1 ml of the upper layer of petroleum ether was dried under vacuum in a new 2-ml tube ...
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf, Hamburg, Germany), the supernatants were collected and residual sample pellets were reextracted with a 200 μL aliquot of the previously used extraction solvent mixture ...
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bioRxiv - Neuroscience 2023Quote: ... After centrifuging the samples for 15 minutes at 5°C at 4000 rpm on bench top centrifuge 5810R (Eppendorf, USA), 600 μL of dichloromethane (bottom layer ...
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bioRxiv - Molecular Biology 2023Quote: ... the rehydrated gel pieces were overlaid with 30 μL of 0.01% ProteaseMAX in 50 mM ammonium bicarbonate and incubated at 37°C for 3 hours with shaking at 800 rpm (Thermomixer, Eppendorf). The digestion reaction was transferred to a fresh tube ...
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bioRxiv - Microbiology 2023Quote: ... cell nuclei were resuspended in 500 μl of 1.2X restriction enzyme buffer and 0.3% SDS (final concentration) and incubated at 37°C with shaking at 300 rpm (Eppendorf Thermomixer). After 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... PFF oligomers were incubated with or without SVD-1a in PBS pH 7.4 for 3 d at 37 °C under quiescent condition in low retention Eppendorf tubes (Eppendorf, GE). Prior to injection ...
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Neuroscience 2023Quote: ... The beads-captured proteins were digested overnight at 37 °C with 0.5 μg trypsin/LysC mix in 25 mM ammonium bicarbonate under vigorous shaking (1200 rpm, Eppendorf Thermomixer). The supernatants were collected ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were initially digested with the addition of 0.1 μg of Lys-C followed by incubation in a ThermoMixer® (Eppendorf) for 6 hours at 37°C with shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... was added and incubated for 30 min at room temperature in the dark while shaking at 1500 rpm (Thermomixer C, Eppendorf). The IAM was quenched with 11 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min-4 h at 76°C in a heat block (speed of 300 rpm; ThermoMixer F1.5, Eppendorf, Germany) (Wagner et al. ...
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bioRxiv - Neuroscience 2024Quote: ... samples were layered on top of the 105 µl cushion and spun at 10,000 g for 20 min at 4°C in a swing out rotor (A-8-11 swing bucket rotor, Eppendorf) to isolate CpxII bound to trans SNARE complexes (SNAREpins ...
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bioRxiv - Neuroscience 2024Quote: ... Neuroepithelial tissue from multiple animals was transferred to this well containing the final dissociation buffer and was cut into small pieces and the suspension was transferred to 14 mL tube and was incubated with gentle shaking at 37°C for 30 minutes on a thermomixer (Eppendorf) by passing 95% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM D-L methionine) for 1 h at 4° C with shaking at 1400 rpm in a benchtop thermomixer 22331(Eppendorf). The beads were washed twice with binding buffer without soybean trypsin inhibitor and suspended in 50 % v/v binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... at a final concentration of 5 mM at 37 °C for 40 minutes with shaking at 800 rpm in a thermomixer (Eppendorf). Alkylation was performed with iodoacetamide (IAA ...
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bioRxiv - Cell Biology 2024Quote: ... 40% glycerol) and boiled at 100°C on a heat block for 20 minutes and then centrifuged at 16,000g (Eppendorf 5415) for 3 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Spheroids were incubated with primary antibodies for 24 hours at 37°C and 300 rpm shaking in a Thermomix (Eppendorf). Spheroids were rinsed with PBS ...
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bioRxiv - Evolutionary Biology 2024Quote: 15mL of a Salten solution (1011 PFU/mL) was distributed into 1.5mL polypropylene tubes and centrifuged for 1 hour at 16,000g at 4°C (Eppendorf Centrifuge 5415R). Pellets were resuspended in 100µL of 100 mM of ammonium acetate (NH4-CH3CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were sorted using a BD FACS Aria cell sorter at 4°C and collected in 0.4 mg/mL BSA in DNA LowBind tubes (Eppendorf, 0030108523). Cells were pelleted at 300 g for 5 minutes at 4°C and manually counted using a Bürker Chamber ...
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bioRxiv - Immunology 2024Quote: ... This slurry was incubated for 1.0 h at 21 °C while shaking at 1,200 rpm on an Eppendorf shaker (Eppendorf, Hamburg, Germany). Excess of CCP4 peptide was removed by washing the beads four times with 1.0 ml PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The cell suspension was centrifuged at full speed (7,197 × g) at 20 °C for 10 minutes in a refrigerated centrifuge (cat. 5430 R, Eppendorf, Hauppauge, NY). The supernatant was discarded ...
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bioRxiv - Biophysics 2019Quote: ... 6 mL of the selection culture in 2 mL centrifuge tubes was pelleted at 5000 rpm for 5 minutes at 4°C in a microcentrifuge (Eppendorf, 5242R). The supernatant was removed except for the last ∼200 µL ...
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bioRxiv - Microbiology 2019Quote: ... Bottles were incubated at 12 °C and shaken at 200 rpm in a New Brunswick Innova43/43R shaker (Eppendorf Nederland B.V.). At regular intervals ...
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bioRxiv - Microbiology 2019Quote: ... The cultures were inoculated at an initial OD660nm of 0.1 and incubated under an air atmosphere and shaken at 200 rpm and at 20 °C in a New Brunswick™ Innova44 incubator (Eppendorf Nederland B.V ...
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bioRxiv - Molecular Biology 2019Quote: ... To reduce non-specific binding of aggregates to the DNA beads, the HSS was centrifuged (14000 rpm, 10 min., 4°C) in a fixed angle microfuge (Eppendorf 5418). The HSS was then split into two 50 µL samples and the DNA-PKcs inhibitor NU-7441 (R&D Systems ...