Labshake search
Citations for Eppendorf :
1 - 50 of 51 citations for Human Cholecystokinin 12 CCK12 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... or 12-well plates (Eppendorf) using standard protocols(Debnath et al ...
-
bioRxiv - Systems Biology 2023Quote: ... 12°C in a ThermoMixer (Eppendorf), and centrifuging for 5 minutes at 12000 rpm ...
-
bioRxiv - Microbiology 2023Quote: ... We typically used a 12 channel P200 (Eppendorf explorer, 4861000724). The following items were gathered before pouring the liquid agarose into a reservoir ...
-
bioRxiv - Molecular Biology 2022Quote: ... and incubated at 37LJC overnight (12 hours) with shaking (Eppendorf, Thermomixer C). An additional 5 µL of same concentration Tryp/LysC was added and samples were incubated for another 4 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... Debris was removed by centrifugation (Eppendorf Centrifuge 5427 R, Rotor FA-45-12-17) 13k rpm ...
-
bioRxiv - Cancer Biology 2020Quote: ... 12 individual colonies per sample were transferred to a 96-well PCR plate (Eppendorf), washed with PBS and stored at −80°C ...
-
bioRxiv - Microbiology 2021Quote: ... and centrifuged at 12 000 rpm for 15 min at 4°C (5804 Eppendorf) to pellet tissue.
-
bioRxiv - Biochemistry 2021Quote: ... cells were seeded into 6-well or 12-well polystyrene coated plates (Eppendorf; EP0030720130, EP0030721012) at a density of 0.3 x 106 cells mL−1 or 0.1 x 106 cells mL−1 ...
-
bioRxiv - Neuroscience 2022Quote: ... was added per well using electronic 12-channel pipettes in speed 3 (e12c-pip; Eppendorf). The plates were temporality incubated at 37°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... were distributed into each well of 12 96-well plates – 4 per background (LoBind Eppendorf). Then 1 μl of uniquely indexed oligo-dT (25 μM ...
-
bioRxiv - Biophysics 2021Quote: ... in particular to adjust reactions volumes to the 8- or 12-channel pipettes (Eppendorf, Hamburg, Germany), repetitive pipette HandyStep (Brand ...
-
Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... The 5 ml overnight culture was centrifuged at 8,000 rpm (Eppendorf MiniSpin F-45-12-11) for three minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... The 24-well plate was shaken for 12 h in an orbital shaker (Eppendorf, New Brunswick, USA) at 30°C and at 200 rpm agitation ...
-
bioRxiv - Microbiology 2020Quote: ... filtered through a 0.45μm filter and then centrifuged for 12 hours at 12,000rpm in a bench-top centrifuge (Eppendorf). Virus was placed on formvar coated copper 400 electron microscopy grids for 5 minutes ...
-
Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Transconjugant cultures were then centrifuged for five minutes at 10,000 rpm (Eppendorf MiniSpin F-45-12-11), resuspended in 1 ml PBS ...
-
bioRxiv - Biophysics 2021Quote: ... The bicelle mixture (~1 mL) was centrifuged (13,400 rpm, Eppendorf F45-12-11 rotor, 4°C, 30 sec) to remove insoluble debris and the supernatant concentrated to ~200 μL using a 0.5 mL 10 kDa MWCO centrifugal filter (Amicon ...
-
bioRxiv - Microbiology 2023Quote: ... A 1 mL sample was centrifuged for five minutes at 10,000 rpm (Eppendorf MiniSpin F-45-12-11), resuspended in 1 mL phosphate buffered saline (PBS ...
-
Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1.5 ml sample was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), resuspended in 1 ml phosphate buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2021Quote: Parasites were cultured in 5% 0+ human erythrocytes (Blood bank, Universitätklinikum Hamburg Eppendorf) in RPMI medium supplemented with 0.5% Albumax at 37°C in an atmosphere of 1% O2 ...
-
bioRxiv - Cell Biology 2023Quote: ... human lung primary cells were processed in 1.5 ml DNA LoBind tubes (Eppendorf), washed in PBS via centrifugation at 400g for 5 min at 4 °C and lysed for 3 min on ice before washing via centrifugation at 500g for 5 min at 4 °C ...
-
bioRxiv - Plant Biology 2023Quote: ... samples (stored for 4-12 weeks) were thawed at room temperature for 15 mins and centrifuged (Eppendorf Centrifuge 5417C) at 11,000 rpm for 14 mins to extract bulk leaf sap ...
-
bioRxiv - Genomics 2023Quote: ... the sample in each 96-well plate was pooled into 12 DNA low-binding 1.5 ml tubes (Eppendorf, 022431021). Genomic DNA (gDNA ...
-
bioRxiv - Microbiology 2020Quote: ... including human and microbial cells (4°C, Eppendorf 5810R centrifuge, 15 min, 3,220 rcf) represented in the “cell pellet” ...
-
bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
-
bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... The samples were immediately cooled on ice and centrifuged for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The cell pellets were stored at −20°C until RNA extraction.
-
bioRxiv - Microbiology 2021Quote: ... The cells were transferred into anaerobic 50 mL reaction tubes inside the anaerobic chamber and harvested outside of the anaerobic chamber for 12 min at 4°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf). The supernatant was discarded inside the anaerobic chamber and the pellet was resuspended in fresh RCM medium to an OD600 of 5-10 ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and then normalized using a 12-channel electronic pipette with a volume range of 10 to 100µL (Eppendorf, Hamburg, Germany) to obtain the concentration required for genotyping.
-
bioRxiv - Microbiology 2019Quote: ... Bottles were incubated at 12 °C and shaken at 200 rpm in a New Brunswick Innova43/43R shaker (Eppendorf Nederland B.V.). At regular intervals ...
-
bioRxiv - Genomics 2022Quote: ... they froze and crushed a single, adult female spider with a pellet pestle (Fisher, #12-141-364) in a Protein LoBind tube (Eppendorf, #022431081) containing 200 uL of Buffer CT ...
-
bioRxiv - Genomics 2022Quote: ... We flash froze adult spiders in liquid N2 and crushed them with a pellet pestle (Fisher, 12-141-364) in a Protein LoBind tube (Eppendorf, 022431081) containing 220 uL of Buffer ATL ...
-
Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... A 1 ml sample of overnight suspension was centrifuged for five minutes at 8,000 rpm (Eppendorf MiniSpin F-45-12-11), the pellet resuspended in 1 ml PBS ...
-
bioRxiv - Genetics 2024Quote: ... The samples were sonicated on ice for 12 to 15 minutes and then centrifuged for 10 minutes at 13200 rpm and 4 °C (Eppendorf centrifuge 5415R) to remove the cell debris ...
-
bioRxiv - Cell Biology 2020Quote: ... falciparum parasites (strain 3D7) were cultured in human RBCs (O+) (transfusion blood, Universitätsklinikum Hamburg Eppendorf, Hamburg). Cultures were maintained at 37° C in an atmosphere of 1% O2 ...
-
bioRxiv - Microbiology 2022Quote: ... falciparum parasites were cultured in human red blood cells (O+ or B+, Universitätsklinikum Eppendorf, Hamburg, Germany) at 5 % haematocrit in an atmosphere of 1% O2 ...
-
bioRxiv - Cell Biology 2023Quote: Human erythrocytes (blood group 0+ donated from the blood bank of the University Medical Center Hamburg-Eppendorf) and trophozoites to be examined were washed twice with incomplete TY-I-S-33 medium (200 x g ...
-
bioRxiv - Cell Biology 2023Quote: ... falciparum 3D7 [129] were cultured in human red blood cells (O+; University Medical Center Hamburg, Eppendorf (UKE)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 (68) were cultured in human red blood cells (O+ or B+, Blood bank, Universitätsklinikum Hamburg-Eppendorf). Cultures were maintained at 37°C in an atmosphere of 1% O2 ...
-
bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein amounts were assessed using a Pierce BCA protein assay kit and spectrophotometer (Eppendorf BioPhotometer). Equal amounts of protein (15 μg ...
-
bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
-
bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
-
bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
-
bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 µl of the samples or blanks were pipetted on the 96 well-plate-based kit containing calibrators and internal standards using an automated liquid handling station (epMotion 5075, Eppendorf) and subsequently dried under a nitrogen stream using a positive pressure manifold (Waters) ...
-
bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
-
bioRxiv - Developmental Biology 2019Quote: ... Plasmids used in initial identification screen for cis-regulatory elements used miniprep scale DNA purification (Zymo Research, D4020 or 5 prime/Eppendorf, FastPlasmid kit) and all subsequent experiments used midiprep scale DNA purification (Qiagen ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...