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Citations for Eppendorf :
451 - 500 of 565 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... with a titer of 1×109 TU/ml were injected into the sub-germinal cavity using an oil hydraulic injection system (CellTram oil, Eppendorf). Before the injection ...
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bioRxiv - Biochemistry 2022Quote: ... at a final concentration of 1 mM for 30 min at 30 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). The reaction was quenched with ammonium bicarbonate at a final concentration of 50 mM for 10 min at 30 °C and 650 rpm ...
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bioRxiv - Immunology 2019Quote: ... six cycles 94°C for 20 sec and a touchdown program of 15 cycles with successive annealing temperature decrement from 65°C to 60°C for 30 sec with 1°C decrement with a final extension at 72°C 10 min performed in a Mastercycler Gradient (Eppendorf). The products were monitored using 1.5 % agarose gel electrophoresis stained with ethidium bromide ...
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in SK buffer (LS buffer, 1% sarkosyl, 500mM NaCl) and solubilized at 22°C for 60 minutes using a ThermoMixer 5350 (Eppendorf) at 500rpm ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... frozen leaves were extracted in 50 mM P buffer (pH 7.5) at a ratio of 1:8 (w/v) and centrifuged (Eppendorf 5417R) twice at 18,000g for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were digested with trypsin (1:50 trypsin:protein ratio) overnight at 37°C with light agitation (450 rpm on a Eppendorf thermomixer). Digestion was stopped by the addition of trifluoracetic acid (TFA ...
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bioRxiv - Biochemistry 2019Quote: ... Varying volumes of viral supernatant were mixed with 5 × 106 to 1 × 107 log phase Jurkat cells in a final volume of 10 ml within a T-25 flask (Eppendorf) and subsequently cultured for 72 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... albeit the injected droplets were collected in fractions of ∼1000 cells (∼27 µl of loaded droplets) in 1 ml LoBind tubes (Eppendorf) pre-filled with 200 µl of mineral oil ...
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bioRxiv - Developmental Biology 2020Quote: ... Pairs of PPFs were isolated manually with forceps from the lateral body wall and from the underlying septum transversum and liver (Video 1) and then each pair placed in a single well in a 96-well plate (Eppendorf) containing 100 μL of media ...
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bioRxiv - Biochemistry 2020Quote: ... chloroform (4:2:1, v: v: v, containing 20 mM ammonium formate. The solutions were then placed into the wells of an Eppendorf twin-tec 96-well PCR plate ...
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bioRxiv - Genomics 2019Quote: ... Deep red-) Single cells were sorted into 5 ul of RLT 1%β-Mercaptoethanol in a 96 well plate (Eppendorf) and frozen at −80°C.
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bioRxiv - Biochemistry 2019Quote: ... and plasma and bone marrow interstitial fluid samples from three pediatric B-ALL patients (B-ALL-1, −2, −3) were processed on an epMotion M5073 automated liquid handling system (Eppendorf) controlled by an EasyCon tablet (Eppendorf) ...
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bioRxiv - Bioengineering 2019Quote: For extract preparation each 4 mL of cell cultures from tp 48h of HDC run 1 were spun down for 10 min at 4000 g and 4 °C (Centrifuge 5804R, Rotor A-4-44, Eppendorf). Pellets were resuspended in fresh 1 mL ‘thylakoid buffer’ (50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: ... 20 g/L tetracycline hydrochloride) was grown in batch mode in a 1 L fermenter (DASGIP 1L Bioreactor, Vaudaux Eppendorf) at pH 7.0 (+/-0.2 ...
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bioRxiv - Microbiology 2020Quote: ... This mixture was centrifuged at maximum speed for 1 min and the supernatant was transferred to a tube containing 300 μL chloroform and Heavy Phase Lock Gel (Eppendorf), inverted for 2 min and centrifuged at maximum speed for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... CD34-) (Supplementary Fig. 1) were sorted at single-cell resolution into a 384-well Eppendorf LoBind PCR plate (Eppendorf AG) containing 1 μl of lysis buffer (50 mM Triethylammonium bicarbonate (TEAB ...
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bioRxiv - Developmental Biology 2021Quote: ... were diluted in water (1:10) and injected into the periplasm of the embryos during cellularization using a FemtoJet express microinjector (Eppendorf). The embryos used in this experiment expressed Utr-Venus and Sqh-mCherry which allowed us to monitor cell shape and actomyosin dynamics ...
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bioRxiv - Biophysics 2020Quote: ... the DNA-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). Droplet formation was induced by manual shaking for about 4 s as described earlier.[26] For the oil-phase ...
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bioRxiv - Molecular Biology 2022Quote: ... Destained gel bands were first cut into small cubes (1-2 mm in each dimension) using a clean scalpel and transferred to new LoBind tubes (Eppendorf). Gel cubes were dehydrated by incubating in 500 μL acetonitrile (ACN ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Genetics 2022Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passes (1 + 17, 2 + 18 … etc.) and dried in a Speed-Vac (Eppendorf).
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bioRxiv - Microbiology 2022Quote: Single cell-stage fertilized wildtype embryos of AB line were injected with a mix of 150 pg Cas9 mRNA and 100 pg stat1a or stat1b sgRNA and (in 1 nL volume) using a Femtojet 4i pressure microinjector (Eppendorf) and a M3301R Manual Micromanipulator (WPI) ...
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bioRxiv - Synthetic Biology 2022Quote: ... as glucose acceptor and 0.1 mM UDP-Glucose as glucose donor in a final reaction volume of 1 mL for 20 min at 30 °C and 800 rpm using a Thermoshaker (Eppendorf). Crude extracts used to test individual assemblies were diluted to an exact protein concentration of 50 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... and streptomycin (10 µg ml−1) at 37° C for 40 min with shaking at 800 rpm in a thermomixer (Eppendorf). Digested medullae were resuspended and the enzymatic reaction quenched by addition of 10 volumes cold CMF-HBSS ...
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bioRxiv - Systems Biology 2023Quote: ... Saponified non-polar metabolites were obtained by modifying the protocol with the addition of 60% KOH (10X) in 50% methanol followed by an incubation at 42°C for 1 h in a thermomixer (300 rpm Eppendorf), instead of the 1 h rotation at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL with 0.1% TFA.
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bioRxiv - Systems Biology 2023Quote: ... and incubated on ice for 1 min before pelleting with a swinging-bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Supernatant was gently removed and ∼50 µl were left behind to increase nuclei recovery ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Freshly laid zebrafish eggs were collected from breeding tanks and 1 nL solution mix was injected using a FemtoJet 4i (Eppendorf) into the yolk before the first cellular division.
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bioRxiv - Microbiology 2023Quote: ... the mucin beads sampled from different time points were first washed with 1 ml filter-sterilized PBS in 2-ml tubes (Eppendorf). After carefully removing the supernatant ...
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bioRxiv - Genetics 2023Quote: ... Samples were mixed at 850 rpm for 1 hour at room temperature on a 96 well MixMate plate mixer (Eppendorf). The beads were concentrated using a magnet and the supernatants removed ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions were collected every three minutes, pooled in two passes (fraction 1 + 17, fraction 2 + 18,…, etc.) and dried in a vacuum centrifuge (Eppendorf).
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bioRxiv - Cell Biology 2022Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passed (1 + 17, 2 + 18 … etc.) and dried in a vacuum centrifuge (Eppendorf). Dried fractions were resuspended in 0.1% formic acid and analyzed on a Orbitrap Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Plant Biology 2023Quote: ... benthamiana leaf tissue 5-days post Agrobacterium infiltration was collected using a leaf disc cutter 1 cm in diameter and placed inside a 2 mL safe-lock tube (Eppendorf). Each biological replicate consisted of 4 leaf discs from the same leaf (approximately 40 mg fresh weight) ...
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bioRxiv - Cell Biology 2023Quote: ... Fractions were collected every three minutes, and fractions were pooled in two passes (1 + 17, 2 + 18 … etc.) and dried in a vacuum centrifuge (Eppendorf). Dried fractions were re-suspended in 0.1% formic acid (FA ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). The peptide sample was then prepared for LC-MS/MS analysis by diluting it to 5 μL by 0.1% TFA.
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bioRxiv - Developmental Biology 2023Quote: ... combined with 3 μg/μL NeuroD:Cre-IRES-GFP construct and 1 μg/μL NeuroD:IRES-GFP vector using a micro injector (Eppendorf Femto Jet). Plasmids were further electroporated into the neuronal progenitors adjacent to the ventricle by discharging five electric pulses (35 V ...
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bioRxiv - Cell Biology 2023Quote: ... and eluted in 40 μL of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μL by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). They were then prepared for LC-MS3 analysis by diluting it to 5 μL by 0.1% TFA ...
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...