Labshake search
Citations for Eppendorf :
651 - 700 of 701 citations for Glucose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in 400 μl 1XHB buffer and transferred to a 2 ml Eppendorf Protein Lo-Bind tube (Eppendorf, UK). 400 μl of 50% Iodixanol solution (Opti-Prep Density Gradient Medium ...
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bioRxiv - Molecular Biology 2020Quote: ... was incubated with 700 μl hexafluoro-2-propanol (HFIP) overnight and divided into 108 μg doses in LoBind reaction tubes (Eppendorf AG). Samples were lyophilized in a rotational vacuum concentrator system connected to a cold trap (both Martin Christ Gefriertrocknungsanlagen GmbH) ...
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bioRxiv - Neuroscience 2021Quote: ... A plasmid DNA solution of 1.4 µg/uL was injected into embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C scope (Leica Microscopes ...
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bioRxiv - Immunology 2022Quote: ... bacterial stocks were grown at 30°C for 2 days to an OD600 of 0.4 approximately 6.3×106CFU/mL (Eppendorf, BioPhotometer; Hamburg, Germany). Bacteria were washed with sterile PBS and aspirated through a 27G needle against the side of the tube multiple times to break up bacterial clumps ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl of the sample was mixed with an equal volume of buffered phenol and 20 μl of 20% SDS in a 2 mL centrifuge tube (Eppendorf, Germany). After adding 0.5g of 2 mm zirconia beads (BioSpec Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted samples were aliquoted in 2 mL tubes and vaporized under vacuum at 40°C using SpeedVac (Eppendorf, Hamburg, Germany). The samples were resuspended and further diluted in sterile water before applying to MBA ...
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bioRxiv - Biophysics 2023Quote: ... were coated with Expi293 cell produced RS2 at 4 µg/mL concentration in 1x PBS (60 µl/well) and incubated for 2 h at 25 °C under gentle shaking condition (300 rpm) on a thermomixer (Eppendorf, USA) and then plate was transferred to 4 °C cold room for overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then exposed to phages at indicated MOIs or incubated untreated for 30 min in a 2 mL Eppendorf tube under shaking conditions (<650 rpm in an Eppendorf ThermoMixer). For TIRF microscopy ...
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bioRxiv - Bioengineering 2023Quote: ... Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf, Germany). Each prepared sample was separated on Hi-pH column (Thermo ...
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bioRxiv - Genetics 2024Quote: ... the samples were washed 3 times with 500 μl Perm wash (centrifugation for 2 minutes at 1600 rpm (Eppendorf centrifuge 5415R)) ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was filtered through a 50 µm filter (Sysmex; 04-004-2327) into a 2 mL microcentrifuge tube (Eppendorf; 022431048). An additional 0.5 mL of homogenization buffer was used to wash the Dounce homogenizer and filter ...
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bioRxiv - Biochemistry 2024Quote: ... #33045-U) and incubated for 2 h at 60 °C at 400 rpm on a thermomixer (Eppendorf ThermoMixer C, Germany, #EP5382000023). Then ...
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bioRxiv - Bioengineering 2024Quote: ... BMP-2 solution was added to FN coated polymers and after 2 h incubation solution was aspirated and collected in Protein LoBind Tubes (Eppendorf™). Enzyme-linked immunosorbent assays (ELISA ...
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bioRxiv - Cell Biology 2020Quote: ... The chondrocytes were incubated with the dye at 37°C for 2 hours at 300 rpm in a ThermoMixer C (Eppendorf, Hamburg, Germany). After incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the medium exchange was conducted under semi-sterile conditions directly at the microscope with a 10 μl microloader tip (Microloader Tip 0,5-10 μl / 2-20 μl, Eppendorf AG, Hamburg, Germany) every two days.
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bioRxiv - Microbiology 2021Quote: ... Bacillus cereus group isolates’ inocula were prepared from overnight cultures (see “Bacterial cultures” section) by adjusting their concentration to 1-2 × 108 CFU/ml using a spectrophotometer (Eppendorf BioPhotometer 6131). A previously established OD-CFU standard curve was used to estimate the CFU/ml based on the OD reading ...
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bioRxiv - Biophysics 2022Quote: ... Ltd., Kanagawa, Japan) controlled by a pneumatic microinjector (IM-11-2, Narishige, Tokyo, Japan) and a micromanipulator (Transferman NK2, Eppendorf, Hamburg, Germany) and transferred into 4 ml RNase-free water (06442-95 ...
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bioRxiv - Biochemistry 2022Quote: ... and samples were vortexed at 1200 rpm for 2-3 hours at room temperature (MixMate ®, Eppendorf South Pacific, Sydney, NSW Australia). Following this ...
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bioRxiv - Neuroscience 2021Quote: ... a whole eye from wild type and mutant adult zebrafish or 100 zebrafish mutant larvae were transferred to 2 ml Eppendorf microcentrifuge tubes (Eppendorf, Hamburg, Germany). 200 μl of 2 M hydroxylamine (pH 8 ...
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bioRxiv - Microbiology 2019Quote: ... were arranged on double-sided sticky tape using a paintbrush and microinjected at the posterior pole of each egg with wAlbB (2×108 bacteria/ml) using a FemtoJet microinjector system (Eppendorf, NSW, Australia). The microinjected eggs were then placed on tissue paper on the surface of artificial manure pats to hatch ...
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bioRxiv - Immunology 2022Quote: ... Purified IgG was digested into F(ab’)2 with 200 μg of IdeS per 10 mg of IgG for 6 hr on a thermal mixer (Eppendorf ThermoMixer C) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... samples were prepared in 96 well microtiter plates by adding 12.5 μL reaction supernatant to 50 μL acetonitrile with 1% v/v trifluoroacetic acid (TFA) followed by centrifugation (2,204 g, 30 min; A-2-DWP rotor, Eppendorf AG, Hamburg, Germany) and transferring of 50 μL centrifugation supernatant into 150 μL MilliQ H2O ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1800 µl of solution from both tubes (batch 1) were transferred to a 2 ml Eppendorf and vortexed (Eppendorf Thermomixer C) for 15 minutes at 2000 rpm (4 °C) ...
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bioRxiv - Genetics 2024Quote: ... placed in an injection arena (9 cm-diameter Petri dish containing a layer of 1% agarose) and injected with ∼60 ng of Pmtra-2 or EGFP dsRNA (negative control) using a FemtoJet microinjector (Eppendorf, Hamburg, Germany). Injectees were maintained in a two-ounce plastic cup with corn leaves until they became adults ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Developmental Biology 2019Quote: ... The embryos were deyolked in ice cold ringer’s solution using micropipette tip and spun at 100g for 2 minutes at cooled table top centrifuge at 40C (Eppendorf® centrifuge 5418R). The supernatant was discarded and the embryo bodies were trypsinized using TrypLE Express (Thermoscientific® ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... of the same volume as the RIPA buffer at 25 °C for 2 h with intermittent mixing (Thermomixer comfort, Eppendorf, Wesseling-Berzdorf, Germany). The digested sample was labelled as the RIPA-insoluble fraction (Ins) ...
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bioRxiv - Microbiology 2023Quote: ... algal cells with the attached bacteria in the treatment with the species consortium were also collected by a pipette from the formaldehyde-fixed suspended sample and transferred into 2 ml safe-lock tubes (Eppendorf, Wesseling-Berzdorf, Germany). To avoid an excessive fixation ...
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bioRxiv - Developmental Biology 2024Quote: ... One- or 2-cell stage embryos were injected with approximately 20 nl of the injection mixture (manually or using an Eppendorf FemtoJet 4x microinjector) and maintained at 20°C until the 64-cell stage ...
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bioRxiv - Systems Biology 2024Quote: ... placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415, Eppendorf, Hamburg, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were spun down (5 min, 800 g•, 2 °C, minimal acceleration and break, Eppendorf 5810 R with swing-×bucket rotor A-4-44). The medium was discarded and the cells were dissociated in 15 mL of ice cold ACK solution (0.15 M NH4Cl ...
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bioRxiv - Cell Biology 2020Quote: ... This solution was centrifuged at 3000g for 2 min and the supernatant was collected and concentrated in a vacuum concentrator in vacuum-alcoholic (V-AL) mode (Eppendorf plus, Eppendorf, Hamburg, Germany). The concentrated crystals were resuspended in 100μl of 1 X PBS and Nuclease Free Water (NFW) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were loaded into a microinjection needle and 50 nL of the ZMEL cell suspension was transplanted into the hindbrain ventricle of anesthetized 2 dpf zebrafish larvae by using an oil-controlled microinjection rig (Eppendorf CellTram 4r Oil, #5196000030) with a Narishige arm ...
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bioRxiv - Bioengineering 2023Quote: ... Transwells® were then incubated at 37 °C and 25 RPM for 2 hrs in a New Brunswich™ Innova® 40 shaker (Eppendorf, Enfield, CT). Samples were collected every hour from the basolateral side ...
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bioRxiv - Microbiology 2023Quote: ... Each flask was aeriated with 2% v/v CO2 enriched air which was controlled by a DASGIP® MX module (Eppendorf AG, Hamburg, Germany). The microalgae starting with an OD750 of 0.1 were cultivated in modified Johnson medium at a pH of 7.5 [49] with 1 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat. No. 022431048) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Flowrate was adjusted at 2 ml/min to collect peptides in 1 ml fractions in low-protein binding Eppendorf tubes (Eppendorf LoBind tubes, cat. no. EPPE0030108.116). The bound complexes were separated from β2 microglobulin and heavy chain using increasing concentration of buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... polar phase were transferred into a new 2 mL reaction tube and dried in a vacuum concentrator (Eppendorf, Concentrator plus, mode: V-AQ, 30 °C) for approximately 4.5 h ...
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bioRxiv - Neuroscience 2020Quote: ... Protein amounts were assessed using a Pierce BCA protein assay kit and spectrophotometer (Eppendorf BioPhotometer). Equal amounts of protein (15 μg ...
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bioRxiv - Biophysics 2020Quote: ... with three ssDNA overhang strands on a bottom partially complementary to the sequence on the magnetic beads (mag2, Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... following the standard protocol in this kit and liquid handling in Eppendorf epMotion 5075 (Eppendorf North America). The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Genomics 2020Quote: ... samples were processed according to the Qiagen PowerMag Microbiome DNA/RNA Isolation Kit protocols on an epMotion 5073 liquid handling robot (Eppendorf). Extracted DNA was quantified using the QIAxpert A260 dsDNA system (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... The reagents were mixed as per the QuikChange II XL Site-Directed Mutagenesis Kit instructions and loaded into a Mastercycler pro S (Eppendorf) with the settings recommended by the manual ...
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bioRxiv - Microbiology 2024Quote: Sample were retrotranscribed in cDNA using 1 µL of RNA with 1 µL of Reverse Transcription Master Mix and 3 µL of RNase-free ultrapure water provided with the kit (Standard Biotools, USA) using a thermal cycler (Eppendorf, Germany) with the following cycles ...
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bioRxiv - Developmental Biology 2019Quote: ... Plasmids used in initial identification screen for cis-regulatory elements used miniprep scale DNA purification (Zymo Research, D4020 or 5 prime/Eppendorf, FastPlasmid kit) and all subsequent experiments used midiprep scale DNA purification (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GoScript Reverse Transcriptase) provided by the kit was added to start the reverse transcription reaction in the thermocycler (Eppendorf Flexide Mastercycler Nexus) for 5 min at 25°C ...