Labshake search
Citations for Eppendorf :
501 - 550 of 659 citations for Estradiol ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... 5 μL nuclease free water) and incubated for 45 min at 37°C at 1000 rpm (Eppendorf Thermomixer) for fragmentation ...
-
bioRxiv - Genetics 2021Quote: ... Filtered nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf) and resuspended in 1 mL Wash buffer (10mM Tris-HCL (pH 7.5) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were pelleted by centrifugation (3220 x g 5 min, Eppendorf 5810R fitted with rotor A-4-81), pellets were recombined with 5 mL pre-chilled MP medium (no carbon) ...
-
bioRxiv - Microbiology 2021Quote: ... we transferred the filter and its contents into a 5-mL polypropylene snap-cap tube (Eppendorf, Hamburg, Germany) because some filter units contained sample water and could not fit the lysis reagents ...
-
bioRxiv - Cell Biology 2022Quote: ... About 5×107 cells as 325 µl suspensions in 1.5 ml safe-lock tubes (Eppendorf AG, Hamburg, Germany) were ruptured by brief pulsed sonication at RT using the ultrasonic homogeniser SONOPULS mini20 ...
-
bioRxiv - Microbiology 2020Quote: ... and subjected to low-speed centrifugation at 20 × g for 5 min (Centrifuge 5810 R, Eppendorf, Hamburg, Germany) to eliminate gross particulate material ...
-
bioRxiv - Microbiology 2022Quote: ... cells were pelleted by centrifugation for 5 min at 15,700 x g using 5415R Microcentrifuge (Eppendorf, Hamburg, Germany) and the supernatant was frozen at −20° C until further use ...
-
bioRxiv - Molecular Biology 2022Quote: ... pH 8.5) with boiling at 95°C for 5 mins with mixing at 1,000 rpm on a ThermoMixer (Eppendorf), cooled on ice ...
-
bioRxiv - Genomics 2023Quote: ... The DNA solution was pelleted by centrifugation at 6,000xg for 5 minutes at 4°C (Eppendorf Centrifuge 5425R). After removing the supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... we centrifuged 1 mL overnight-cell culture in a 1.5 mL Eppendorf tube at 7’500 rcf for 5 min (Eppendorf, tabletop centrifuge MiniSpin plus with rotor F-45-12-11 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were centrifuged at 4000 rpm × 5 min at 4°C in a pre-cooled microcentrifuge (Eppendorf 5415R) and the supernatant was collected ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were centrifugated at 10.000xg at room temperature for 5 min (centrifuge MiniSpin® Eppendorf, ClearLine, Venice, Italy) and stored at −80°C until further use.
-
bioRxiv - Microbiology 2023Quote: ... and the cells were incubated at 37°C/ 5% CO2/ 1% O2 (Galaxy 170 R/ New Brunswick/ Eppendorf). Cells were cultured for up to 14 days with bi-weekly media change ...
-
bioRxiv - Bioengineering 2023Quote: ... the nanoparticles were collected via centrifugation at 12000 rpm (13523 x g) for 5 min (Centrifuge 5420, Eppendorf), and then washed with acetone and distilled water 3 times ...
-
bioRxiv - Microbiology 2024Quote: ... Each 9 mL suspension was centrifuged at 9,000 × g for 5 min (Centrifuge 5810 R, Eppendorf, Hamburg, Germany). DNA extraction was performed from the pellet using the GeneJET Genomic DNA Purification Kit (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... Experiments on the seconds to minutes time scale were performed at room temperature in 96-well Protein LoBind plates (Eppendorf, Hamburg, Germany).
-
bioRxiv - Microbiology 2021Quote: ... 10 µL was transferred to a PCR-clean polypropylene V bottom 96-well plate and covered with a silicone seal (Eppendorf, Mississauga, ON). Reactions were incubated at 29°C for 16 hours and final reactions stored at -20°C ...
-
bioRxiv - Neuroscience 2021Quote: ... The cells stably expressing H-Luc-NLS were plated on a black flat glass bottom 96-well plates (Eppendorf; tissue culture treated). They were incubated with 1 μM MaPCahigh AM dyes and 0.04% Pluronic-F127 for at least 2 h in 100 μL at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... We included an overnight digestion step in which tissues were lysed in a 96-well PCR plate at a constant temperature of 55° C on an Eppendorf Mastercycler thermal cycler (Eppendorf, Hamburg, Germany), and we included 10 uL Proteinase K solution ...
-
bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
-
bioRxiv - Biochemistry 2021Quote: ... Resulting peptides were desalted by reverse-phase purification using a SepPak 96-well plate and dried by vacuum centrifugation (Concentrator plus, Eppendorf, Hamburg, Germany).
-
bioRxiv - Microbiology 2021Quote: ... the plate was returned to the magnet and for 2 minutes and 48 μl of purified DNA was transferred to a fresh 96-well PCR plate (Eppendorf 0030 128.648).
-
bioRxiv - Microbiology 2021Quote: ... The qPCR experiment was performed in 96-well PCR plate using Eppendorf Mastercycler® RealPlex with SYBR Green PCR Master Mix (Eppendorf, USA) as per the universal SYBR Green qPCR protocol where fluorescent product is detected during the last step of each cycle ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.1% formic and mixed with the now washed Oligo R3 Resin and allowed to shake on a plate shaker (Eppendorf, Thermomixer Comfort) for 5 minutes at 800 RPM ...
-
bioRxiv - Neuroscience 2019Quote: ... Reverse transcription of GEMs was performed in a Bio-Rad PTC-200 thermal cycler with a semi-skirted 96-well plate (Eppendorf, #0030 128.605): 53°C for 45 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5.5 × 106 cells were suspended in 5.5 ml mTeSR1 per well of 6-well ultra-low attachment plates (Coring Costar) and cultured overnight in an orbital shaking CO2 incubator (Eppendorf New Brunswick) at 95 rpm ...
-
bioRxiv - Immunology 2020Quote: ... cells were sorted from cryopreserved PBMC and T cell lines as positive controls using CD1b tetramers loaded with GMM or purified SGL into single wells of a 96-well PCR plate (Eppendorf, Hamburg, Germany), which contained 5 μl of sort buffer consisting of (1X Qiagen One-step RT PCR Buffer (Qiagen ...
-
bioRxiv - Cell Biology 2021Quote: ... the Matrigel coat was broken by vigorously pipetting up and down and the healthy organoids were transferred to a 60mm low attachment culture plate (Eppendorf, cat # 003070119). The plates were then moved to a 37°C incubator and to a Celltron benchtop shaker for CO2 incubators (Infors USA ...
-
bioRxiv - Neuroscience 2023Quote: ... The suspension was centrifuged at 1500 rpm for 5 minutes at 4°C and the pellet was resuspended in cold growth factor reduced Matrigel and aliquoted (15 μL per well) in a 48 well plate (Eppendorf, Cat#0030723113). Matrigel was let to polymerize for 30 minutes at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... we removed the 96-microwell plate from the anaerobic chamber and incubated the cultures aerobically with agitation at 900 rpm (Eppendorf ThermoMixer FP) at 4 °C for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... the Matrigel coat was broken by pipetting up and down and the healthy organoids were transferred to a 60mm low attachment culture plate (Eppendorf, cat # 003070119). The plates were then moved to a 37°C incubator and to a Celltron benchtop shaker for CO2 incubators (Infors USA ...
-
bioRxiv - Cell Biology 2023Quote: ... GEMRT was performed in a Bio-Rad PTC-200 Thermal Cycler with semi- skirted 96-Well Plate (Eppendorf P/N 0030 128.605): 53 °C for 45 min ...
-
bioRxiv - Microbiology 2019Quote: ... 100 mM NaCl for 5 days at 37°C with constant shaking at 500 rpm in a thermomixer (Eppendorf). The formation of amyloid filaments was followed by Thioflavin T (ThT ...
-
bioRxiv - Immunology 2021Quote: ... The culture supernatant containing the virus was centrifuged at 4,500 g for 5 min at 4°C (Eppendorf 5810R) and stored at -80°C ...
-
bioRxiv - Genomics 2021Quote: ... 0.6 mM DTT) and pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Pulverized frozen tissue and pelleted nuclei from gentleMACS M-tubes were each split into two further aliquots ...
-
bioRxiv - Neuroscience 2020Quote: ... Isolated brain nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA ...
-
bioRxiv - Neuroscience 2019Quote: ... before gently spinning down the PR droplets at 4,000 rpm (3220 x g) for 5 min (Eppendorf 5810 R). Eppendorf tubes with phase separated samples were dispensed in 50 ml Falcon tubes and spun down in a swinging-bucket centrifuge ...
-
bioRxiv - Systems Biology 2021Quote: ... 5 ml of working volume was used in the batch culture at 37°C on a rotary shaker (Eppendorf). The pH of the growth medium was set to 7 ± 0.05.
-
bioRxiv - Genomics 2022Quote: ... Multiple 20µm tissue curls were cut per tissue and collected in pre-cooled 5 ml tubes (Eppendorf, Hamburg, Germany) ensuring no thawing while transferring and stored on dry ice until processing ...
-
bioRxiv - Genomics 2019Quote: ... Cells were thawed and pelleted in a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Cell pellets were resuspended in 250 μl nuclei permeabilization buffer (10 mM Tris-HCl pH 7.4 (Sigma) ...
-
bioRxiv - Cell Biology 2019Quote: ... Approximately 5– 10 nl of cell suspension was microinjected into the pericardial cavity of the embryo using CellTramVario (Eppendorf), Injectman Ni2 (Eppendorf ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). All used cell lines were confirmed to be mycoplasma free by using MycoAlert Mycoplasma Detection Kit (Lonza).
-
bioRxiv - Genetics 2021Quote: ... A study pool quality control (SPQC) sample was prepared by combining 5 µL of supernatant from each sample into a 1.5 mL tube (Eppendorf). Stable Isotope Labeled (SIL ...
-
bioRxiv - Genomics 2019Quote: ... Isolated brain nuclei were pelleted with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). Nuclei pellets were resuspended in 1 ml nuclei permeabilization buffer (5 % BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were put on ice for 30 min and precipitates were spun down for 5 min at 20,000 g at 4°C in a tabletop centrifuge (Eppendorf). After one wash with ice-cold acetone ...
-
bioRxiv - Cell Biology 2021Quote: ... spun at 1,000 x g at −9°C for 5 minutes then aliquoted into lo-bind 2ml centrifuge tubes (Eppendorf) and snap frozen in liquid nitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) to remove intact cells and debris ...
-
bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors and PMSF (10 minutes on ice) and centrifuged at 13,300 rpm for 5 min at 4OC (Centrifuge 5810 R; rotor A-4-81; Eppendorf). The supernatant (whole cell lysates ...
-
bioRxiv - Microbiology 2022Quote: Sheep or horse erythrocytes were washed three times with sterile PBS (pH=7.4) at 300⍰×⍰g for 5⍰min in a refrigerated centrifuge (Eppendorf). These washed erythrocytes suspension was lysed with 0.1% saponin and the concentration of oxy-hemoglobin in this preparation was determined using the QuantiChrom™ Hemoglobin Assay Kit (BioAssay Systems) ...
-
bioRxiv - Microbiology 2022Quote: ... supernatants containing planktonic bacteria were removed from cultures and bacteria were pelleted down by centrifugation at 13,000 rpm x 5 min in a refrigerated micro-centrifuge (Eppendorf). In another set of experiments ...