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Citations for Eppendorf :
51 - 100 of 373 citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Unbound lysate was removed and the Dynabeads transferred to 2 ml LoBind protein tubes (Eppendorf). Beads were washed five times with 2% SDS wash buffer (150 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lysates were cleared by a 30 min centrifugation at 3000 rpm (Eppendorf Centrifuge 5810 R) at 21 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Unbound lysate was aspirated and the beads transferred to a 2 ml LoBind protein tube (Eppendorf) using 2% SDS wash buffer (150 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... 200 µl of cleared lysate was transferred to a new 2 ml tube (Eppendorf 0030 120.094) and used for the robot-assisted protocol ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates were centrifuged at 14,000 g for 10 minutes on a 5425R benchtop centrifuge (Eppendorf) to clarify the samples ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted lysates in 60% acetonitrile/0.1% formic acid were dried by vacuum centrifugation (Eppendorf; Concentrator Plus) at 45°C.
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bioRxiv - Systems Biology 2022Quote: ... Lysates were transferred into a reaction tube (Eppendorf low binding tube, 2 mL, Eppendorf, Hamburg, Germany), followed by addition of 500 µL ice-cold chloroform (CHCl3 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, Eppendorf #A-4-81 rotor) to remove intact cells and debris ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were centrifuged at 4 °C for 30 min at max speed (Eppendorf 5910R, 20133 rcf). The supernatants ...
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bioRxiv - Immunology 2021Quote: ... The obtained lysates were centrifuged for 20 min at 21,000 g and 4°C (Centrifuge 5424 R, Eppendorf) to precipitate insoluble fractions ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at 4°C at 3220 g for 15 min (Eppendorf #A-4-81 rotor) to remove intact cells and debris ...
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bioRxiv - Genomics 2020Quote: ... The lysates were then incubated at 37°C for 10 minutes at 1150 rpm shaking on a Thermomixer (Eppendorf). Lysates were cleared by centrifugation at 15,000g for 2 minutes ...
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bioRxiv - Systems Biology 2021Quote: ... In brief: crude cell lysates were transferred from source plates to 384 well v-bottom PCR Plates (Eppendorf #951020702) assay plates ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lysates were transferred to a new tube and were centrifuged for 2 min at full speed (Eppendorf centrifuge) and the supernatant was carefully mixed with 1 volume of 70% HPLC-grade ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Two ml of phage lysate with 109 PFU/ml were centrifuged at 14,000 rpm (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was centrifuged at 4°C (3220 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) to remove intact cells and debris ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
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bioRxiv - Molecular Biology 2022Quote: ... the resulting cell lysates were passed through a 20 G needle 100 times or heated using a Thermomixer (Eppendorf) at 55°C for 10 minutes with 1000 rpm agitation prior to the conventional RNA purification procedure in accordance with the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Genomics 2022Quote: ... possibly remaining parts of the tissue sections were scraped off with the pipette tip and the lysates were collected in nine separate DNA LoBind tubes (Eppendorf). The wells were washed once with 40 μL of lysis buffer and the washing solution was pooled with the lysate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then centrifuged at 14,000xg for 10 minutes to pellet insoluble debris and the resultant clarified lysate was transferred to a fresh protein LoBind tube (Eppendorf).
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation at 20817 × g for 10 minutes to remove all matrix particles the resulting lysate was transferred to a fresh 2.0 mL Lo-Bind tube (Eppendorf, Germany). DNA was isolated from the lysate using two approaches ...
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bioRxiv - Microbiology 2024Quote: ... The crude cell lysate was refined by centrifuging at 18514 g for 40 min (Fixed angle rotor, 5810-R Centrifuge, Eppendorf). The clarified lysate was applied to a 5 mL HisTrap™TALON™ crude cobalt column (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was spun down at 13600 rpm for 10 minutes at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysate was spun down at 13600 rpm for 10 min at 4°C on a table-top microcentrifuge (Eppendorf, Hamburg, Germany). 1 μl was used to determine protein concentration using Bradford dye at 595 nm wavelength ...
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bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
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bioRxiv - Biochemistry 2023Quote: ... Samples were thawed and Urea was added to a final concentration of 8 M urea and the protein lysate was transferred to 2mL snap-cap centrifuge tubes (Eppendorf, Hamburg, Germany) with 0.1 mm zirconia beads and bead beat in a Bullet Blender (Next Advance ...
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bioRxiv - Molecular Biology 2023Quote: ... we favoured magnetic beads to perform DNA extraction and lysates were processed for DNA extraction using the Macherey-Nagel™ NucleoMag® Tissue kit on an epMotion® 5075vt (Eppendorf, Hamburg – Germany). Volumes on the first binding step were adjusted to the starting volume of lysis buffer accordingly ...
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bioRxiv - Biophysics 2021Quote: ... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
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bioRxiv - Immunology 2019Quote: ... and concentrated to ~1 μg μl-1 using a Concentrator 5301 (Eppendorf).
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bioRxiv - Biochemistry 2021Quote: ... diluted 1:1 with F-actin buffer and cleared by centrifugation (Eppendorf centrifuge 5424R ...
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bioRxiv - Biophysics 2021Quote: ... We put 500 mL of log phase density (1×106-1×107 cells/mL) cells into 1 mL disposable cuvettes (UVette, 952010051, Eppendorf AG, Hamburg, Germany). We measured the OD600 every hour for 7 hours in each of two cuvettes for each cell line ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × Denhardt’s solution (Eppendorf, 0032007.155). Hybridization was performed with a double DIG-labeled locked nucleic acid (LNA ...
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bioRxiv - Microbiology 2022Quote: ... 1% oxygen (hypoxic; Eppendorf CellXpert incubator), or 0% oxygen (anoxic ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The sequencing failed for 1 sample and 1 sample was damaged during the travel (Eppendorf tube burst), decreasing our total sample size to 41 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...
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bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Developmental Biology 2021Quote: ... A Femtotip microcapillary (1 μm tip diameter, Eppendorf, Germany) was mounted onto a Femtojet pump and micromanipulator (Eppendorf) ...
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bioRxiv - Biochemistry 2019Quote: ... Dilutions (1:2) were performed using EP Motion (Eppendorf) and transferred to cells using the Tecan Freedom Evo ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were washed twice for 3 min at 400 RCF at room temperature with PBS supplemented with 1% glucose and 1% pen-strep (Eppendorf 5702R, Hamburg, Germany). Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... and agitated (1,300rpm, 30 min, 1°C; Thermomixer Comfort, Eppendorf). Solubilized membrane proteins were obtained in the supernatant from this agitated suspension by centrifugation (21,000g ...
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bioRxiv - Immunology 2020Quote: ... The pH of the eluate (1 mL per Eppendorf tube) was neutralized (125 µL of Tris 1M ...