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Citations for Eppendorf :
451 - 500 of 522 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: All cells were kept at 37°C and 5% CO2 in a Galaxy 170 R humidified incubator (Eppendorf, Hamburg, Germany). They have also been regularly tested for mycoplasma contamination by examining the samples for extracellular DNA staining with SiR-DNA (100 nM ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Microbiology 2022Quote: ... Neutrophils were then co-incubated with bacterial strains at an MOI of 1:1 ratio in RPMI 1640 cell culture medium (HIMEDIA) and incubated at 37⁰C in an animal tissue culture incubator (Eppendorf). The same method was performed for macrophage RAW 264.7 cell line in the DMEM medium (HIMEDIA) ...
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bioRxiv - Microbiology 2023Quote: ... 2ml of cells were collected at different time points and was centrifuged at 2,348g (FA-45-24-11, Eppendorf rotor) for 5 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the growth medium was aspirated and Cultrex droplets were gently dislodged into the cell recovery solution (Cat. No. 354270, Corning and transferred to Eppendorf tubes using wide-bore 1 ml pipette tips ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: ... gating was established and single cells were sorted (130 micron nozzle assembly) into low-bind 96 well PCR plates (Eppendorf twin.tec LoBind ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 × 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Genetics 2023Quote: Zebrafish embryos were collected and injected as previously described (Rosen et al., 2009) at one-cell stage using a FemtoJet Injector (Eppendorf) or PV820 injector (WPI ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Cell Biology 2023Quote: ... guided through a microinjection needle holder was placed just above the cells of interest by means of a microinjection micromanipulator InjectMan NI2 (Eppendorf). LED590 (ThorLabs M590F2 ...
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bioRxiv - Molecular Biology 2024Quote: ... was injected into the cytoplasm of 1-cell embryo in a droplet of M2 medium using a FemtoJet microinjector (Eppendorf) with constant flow settings ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with both TAP amplifications in a final volume of 1 ml into 96 deep well plate (Eppendorf), and after 7 days of expression mAb-containing supernatants were harvested by centrifugation.
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bioRxiv - Microbiology 2024Quote: ... A volume of 1 nL containing 500 ng/μL mRNA coding for each of these proteins was injected into one-cell stage embryos using the FemtoJet 4i microinjector (Eppendorf). H2O was used as reference control.
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bioRxiv - Developmental Biology 2024Quote: ... One nL morpholino (dissolved in 0.2 M KCl with 0.05% phenol red at 3 mg/mL) was microinjected into haploid embryos at the 1 or 2-cell stage using FemtoJet and InjectMan NI2 (Eppendorf).
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bioRxiv - Developmental Biology 2024Quote: ... and cells were sorted using a BD FACS Aria cell sorter at 4°C and collected in 0.4 mg/mL BSA in DNA LowBind tubes (Eppendorf, 0030108523). Cells were pelleted at 300 g for 5 minutes at 4°C and manually counted using a Bürker Chamber ...
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bioRxiv - Genomics 2024Quote: ... and washed in nuclease-free H2O before proceeding to MNase digestion with 1-2U of MNase for 3×106 cells that went on for 1-hour at 37C while shaking in a thermomixer (Eppendorf) at 550rcf ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Biophysics 2021Quote: ... The monolayer was formed by seeding the suspended cells in the centre of a 35 mm diameter glass-bottom Petri dish (Eppendorf®). After the formation of the confluent monolayer ...
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bioRxiv - Cell Biology 2020Quote: ... The micromanipulator was installed on the microscope allowing for immediate cell microscopy after the injection and was connected to the microinjector FemtoJet (Eppendorf, Germany). The microinjection was performed at 20-30 hPa injection pressure ...
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bioRxiv - Zoology 2019Quote: ... Testicular and ovarian cell suspension was loaded into the glass capillary attached to MN-153 micromanipulator (Narishige) and FemtoJet® 4x injector (Eppendorf). Triploid recipients were injected by approximately 3000-5000 of testicular cells (TC group ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Escherichia coli TG1 cells were electroporated (2.5 kV, 25 μF, 200 Ω) with the linked products using an Eppendorf Eporator® instrument (Eppendorf). Colony PCR and sequence analysis (GENEWIZ ...
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bioRxiv - Cancer Biology 2020Quote: ... Single LRCs and nLRCs were isolated as single PKH-positive/DAPI-negative and PKH-negative/DAPI-negative cells using a micromanipulator (Eppendorf PatchMan NP2, Eppendorf, Germany) or flow cytometric activated cell sorting ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Genetics 2022Quote: ... solution containing capped mRNAs (green fluorescence protein [GFP]mRNA or mutant pls1 mRNA or wildtype pls1 mRNA) was injected into the zebrafish embryos (one-cell stage) using FemtoJet 4i (Eppendorf, Germany). Embryos at different developmental stages were preserved and collected for subsequent experiments
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bioRxiv - Systems Biology 2019Quote: ... and the bacterial cells were harvested by centrifugation (swing-out rotor A-4-44, Eppendorf; 3220 g, 8 min, 30°C). The cell pellet was resuspended in the mineral medium and centrifuged again ...
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bioRxiv - Microbiology 2022Quote: ... Targeted cells were collected using capillary-based micro-manipulation with glass capillaries containing an inner diameter of 8µm (Eppendorf, Hamburg, Germany). Capillaries were pre-coated with a sterile 2% BSA solution to prevent the gametes from sticking to the glass capillary surface ...
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bioRxiv - Synthetic Biology 2023Quote: ... then 1 mL aliquots were placed in triplicate in plastic-bottomed black 24-well plates (Eppendorf Cell Imaging Plates ref. 0030741005), and the plates were placed onto a custom made light plate apparatus (LPA)27 to control blue light illumination in each well (λ = 460 nm ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Following that, the cells were washed thrice with 1mL Dulbecco’s DPBS and centrifugation (600 ⍰ g, 5 min, room temperature)(Eppendorf, cat # 5424R). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... Cell injection was performed by using a micromanipulator (Inject Man) and an oil-driven microinjector (CellTram vario) (both Eppendorf, Wesseling, Germany).
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bioRxiv - Plant Biology 2023Quote: ... was added to frozen cell pellets and incubated for 10 min at 65°C and 1000 rpm in a Thermomixer (Eppendorf, Germany). The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...
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bioRxiv - Bioengineering 2024Quote: ... the EV-conditioned medium was collected in polypropylene tubes and centrifuged at 300 ×g for 11 min followed by 2000 ×g for 22 min to pellet down apoptotic bodies and cell debris using an Eppendorf 5810 R 15 amp centrifuge (Eppendorf, Germany). The supernatant was transferred to polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet MVs using a Sorvall MX 120+ micro-ultracentrifuge (Thermo Scientific ...
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bioRxiv - Genomics 2024Quote: ... The grown bacterial cells were subsequently centrifuged at 5000 G for 10 min using a 5920 R centrifuge (Eppendorf, Hamburg, Germany) washed in 1 mL 0.9% NaCl and subsequently centrifuged again at 12000 rpm for 3 min ...
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bioRxiv - Immunology 2021Quote: ... cell populations were distinguished by WGA and DRAQ5 signals and with individual cells sorted into each well of a 384-well plate (twintec™ PCR plates, Eppendorf, Germany) containing 2.3 µl lysis buffer (Picelli et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Neuroscience 2021Quote: ... The cells stably expressing H-Luc-NLS were plated on a black flat glass bottom 96-well plates (Eppendorf; tissue culture treated). They were incubated with 1 μM MaPCahigh AM dyes and 0.04% Pluronic-F127 for at least 2 h in 100 μL at 37°C ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 30 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
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bioRxiv - Immunology 2022Quote: ... was added to the cells and they were incubated for 45 min at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). The liquid in the plate was removed using a vacuum manifold ...
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bioRxiv - Immunology 2019Quote: ... 1*106 T cells/condition were harvested 72h after α-CD3/α-CD28 activation and transferred to DNA Lo-binding Eppendorf tubes (Eppendorf). Cells were washed once with PBS and supernatant was completely removed ...
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bioRxiv - Biophysics 2020Quote: ... The cell suspension was subsequently centrifuged for 30 min at 4°C and 3250 × g (Eppendorf Swing-bucket rotor A-4-62). The supernatant was discarded ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were recovered from each well by centrifugation at 6000 g for 15 minutes using an Eppendorf 5424 Microcentrifuge (Eppendorf, Hamburg, Germany). The cell pellet was dissolved in 250 µL of DMSO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 100 ng/μL of Cas9 mRNA and 25 ng/μL of sgRNA was injected into Japanese medaka 39 embryos at the one-cell stage using Femto Jet (Eppendorf, Hamburg, Germany) and GD-1 needles (Narishige ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Genetics 2020Quote: ... Approximately 3 nl of RNP complex were injected into the animal pole of one-cell stage embryos (50-250 embryos/experiment) using a Femtojet 5247 microinjector (Eppendorf, Hamburg, Germany) under a Nikon DS-Ri2 stereomicroscope ...