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Citations for Eppendorf :
601 - 650 of 659 citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was then transferred to a pre-chilled electroporation cuvette and after approximately 5 – 10 min electroporated in the eporator electroporator (Eppendorf) set to 1850 V ...
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bioRxiv - Cell Biology 2023Quote: ... After a 5-minute incubation the protein concentration was determined through measurement of the OD595 with a photometer (BioPhotometer plus, Eppendorf). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We collected these bacteria into KK2 with a sterile loop and diluted them to 1.5 OD600 in KK2 (∼5 x 108 cells, measured with an Eppendorf BioPhotometer). We used these bacteria as food for amoebas in our experiment and repeated this process anew for each of the three replicate experiments.
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bioRxiv - Neuroscience 2023Quote: Neurons remained in medium and were incubated in ∼1% O2 (5% CO2, 37°C) for 360 min in a triple-gas incubator (Eppendorf) and then returned to normoxic conditions (21% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... is harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... at a final concentration of 5 mM at 37 °C for 40 minutes with shaking at 800 rpm in a thermomixer (Eppendorf). Alkylation was performed with iodoacetamide (IAA ...
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bioRxiv - Cell Biology 2024Quote: ... The culture was maintained in a humidified atmosphere containing 5% CO2 at 37 L in a cell incubator (Eppendorf, Germany). The medium was changed daily ...
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bioRxiv - Genomics 2021Quote: ... Reactions were prepared in triplicate in a 96-well optical plate using the epMOTION® M5073 automated liquid handling system (Eppendorf, Cat. no. 5073000205D). qPCR conditions were as follow ...
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bioRxiv - Cell Biology 2021Quote: ... as previously stated) they were transferred (three-quarters of a T25 flask/well) to non-adherent 24-well plates (Eppendorf, Hamburg, Germany, Cat #0030 722.019). Vero cells were plated in 24-well plates and then exposed to ZIKV at an MOI of 1 ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Biophysics 2019Quote: ... 6 mL of the selection culture in 2 mL centrifuge tubes was pelleted at 5000 rpm for 5 minutes at 4°C in a microcentrifuge (Eppendorf, 5242R). The supernatant was removed except for the last ∼200 µL ...
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bioRxiv - Biochemistry 2021Quote: ... After suspension it was subjected to centrifugation for 5 minutes at 5000 rpm in an Eppendorf 5804R centrifuge (Eppendorf, Hamburg, Germany) after which the supernatant was decanted into the waste ...
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bioRxiv - Bioengineering 2021Quote: ... CsupADH_17286 and HzeaADH7 were introduced into Agrobacterium tumefaciens GV3101 strain (MP90RK) by electroporation (1700 V mm-1, 5 ms, Eppendorf 2510). A viral silencing suppressor protein P19 was introduced into GV3101 strain as well in order to inhibit the host cells’ transgene silencing apparatus and extend transgene expression over a longer period of time with a higher degree of expression (Canto et al ...
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bioRxiv - Biochemistry 2022Quote: ... After 5 min incubation at room temperature (RT) the sample was centrifuged (8 min, RT, 180 x g, Eppendorf centrifuge 5810R) to separate proteoliposomes from the non-incorporated protein and detergent ...
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bioRxiv - Microbiology 2020Quote: ... and 100 U/mL penicillin/streptomycin (Himedia, Mumbai India) under conditions of 5% CO2 and humidified air at 37°C (Eppendorf India).
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bioRxiv - Cell Biology 2019Quote: ... The resin was pelleted down by centrifugation at 4°C at 1258 g for 5 min (Eppendorf #A-4-81 rotor) and washed with 25 ml ice-cold binding buffer [10 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... overnight cultures in LB were spun down via centrifugation for 5 minutes at 4000 rpm at room temperature (Eppendorf 5810 R). The supernatant was filtered twice (Medical Millex-GS Filter ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were dislodged from a T75 flask and pelleted via centrifugation at 1300 rpm for 5 minutes at 20 °C (Eppendorf 5804R). The supernatant was aspirated ...
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bioRxiv - Neuroscience 2023Quote: ... Following that, the cells were washed thrice with 1mL Dulbecco’s DPBS and centrifugation (600 ⍰ g, 5 min, room temperature)(Eppendorf, cat # 5424R). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was then concentrated on a 1.2 µm size meshed mixed cellulose ester membrane (Merk) and pelleted using centrifugation for 5 minutes at 1000G and 20°C with a swinging bucket centrifuge (Eppendorf 5427R).
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bioRxiv - Cell Biology 2023Quote: ... or at 37°C/5% CO2/1% O2 in a nitrogen-controlled hypoxic incubator (New Brunswick Galaxy 170R, Eppendorf, Hamburg, Germany). Prior to media changes ...
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bioRxiv - Cancer Biology 2023Quote: ... transferred all the tissue fragments from the Petri dish into a clean 5 mL low-binding tube (Eppendorf, cat. no. 0030108.310), and rotated the tube at 20 rpm at room temperature for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were kept at 37 °C and 5% CO2 in a humidified incubator (Galaxy 170 S CO2, Eppendorf, Hamburg, Germany) and regularly passaged at the confluence of 70-80% ...
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bioRxiv - Microbiology 2024Quote: ... 50 mL culture was centrifuged for 5 min at 18 °C and 3,220 g (5810 R swing-out centrifuge; Eppendorf, Hamburg, Germany) and the supernatant was removed by vacuum suction ...
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bioRxiv - Microbiology 2024Quote: ... Pellets were washed with cold 70% ethanol and centrifuged at 19,000 × g for 5 min at 4°C and air dried for 15 min in the Centrifugal Vacuum Concentrator 5301 (Eppendorf, USA). The quality of RNA was assessed using a NanoDrop (ND-1000 ...
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bioRxiv - Microbiology 2024Quote: ... The stationary pre-cultures were then pooled and washed by centrifugation (5 min, 3,220 g and 4 °C) using a 5810 R swing-out centrifuge (Eppendorf, Hamburg, Germany). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of the lipid-containing lower organic phase was spotted on a MALDI target plate (Prespotted AnchorChip 96 Set for Proteomics II; Bruker Daltonics/Eppendorf; washed peptide-free with 2-propanol) pre-spotted with 1 µl of 9-aminoacridine (10 mg/ml dissolved in acetone:water 9:1 (v/v) ...
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bioRxiv - Microbiology 2019Quote: ... and extension at 72°C for 1 min followed by final extension was conducted at 72°C for 5 min using a PCR minicycler (Eppendorf Ltd., Germany). After amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... the heads were cleaned with alcohol and a 1μl single cell suspension (5 x 106 cells/ml) was manually injected directly into the left cerebellar hemisphere using a glass microcapillary (Eppendorf, 5195 000.079) controlled with an Eppendorf CellTram Vario manual microinjector ...
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bioRxiv - Microbiology 2020Quote: ... The overnight culture was diluted in Mueller Hinton Broth (MHB) based on the OD600 to obtain 5 × 105 CFU/mL using a BioPhotometer D30 (Eppendorf, Hauppauge, NY). An OD600 of 1 was assumed to be equal to 8 × 108 CFU/mL.
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bioRxiv - Cancer Biology 2020Quote: ... sonicated for 15 min and vigorously mixed at 5°C for 30 min with 2000 rpm on a ThermoMixer (Eppendorf, Hamburg, Germany). Samples were centrifuged for 5 min at 18000 rpm ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We separated the tissue from the buffer and reproductive fluid by centrifuging at 4ºC for 5 min at 3500 rpm (Eppendorf Centrifuge 5702RH, USA), pipetted the supernatant from each tract region into microplate wells (CellStar 96 Well Cell Culture Plate ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Microbiology 2020Quote: ... we centrifuged a 0.5-mL sample of culture in the exponential phase at 7000 rpm for 5 min in a Benchtop centrifuge (5424 Eppendorf, Hamburg, Germany). We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR ...
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bioRxiv - Genomics 2021Quote: ... then harvested with a sterile cell scraper while at -20°C and transferred to −20 °C cold 5 mL centrifuge tubes (Eppendorf Lo-Bind). After centrifuging the cell-extracts in a 4 °C centrifuge for 5 min at 2000 x g ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was pelleted down by centrifugation at 4°C (1258 g for 5 min, A-4-81 rotor, Eppendorf, Enfield, CT) and washed with ice-cold 50 mL Binding buffer composed of 10mM imidazole (pH 7.4) ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial mixtures were diluted 1:1000 into LB with or without 1% DMSO (v/v) and incubated standing at 37°C in 5% CO2 at atmospheric oxygen (normoxic; Eppendorf CellXpert incubator), 1% oxygen (hypoxic ...
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bioRxiv - Cancer Biology 2023Quote: For automated dual luciferase reporter assay 2-2.5 x 104 HEK 293 T cells were seeded per 96-well by the liquid handling system epMotion 5075 (Eppendorf, Hamburg, Germany). HEK 293 T cells were transfected with 50 ng/well reporter vector with or without 3′UTR and 200 ng/well pSG5 empty vector or pSG5-miR-142-5p expression plasmid ...
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bioRxiv - Genomics 2023Quote: ... The bone pieces in 5 ml tube of DNA Lobind were treated pre-digestion for 15 minutes at 900 rpm in a Thermomixer (Eppendorf, Hamburg, Germany) with the lysis buffer (2 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... the gRNA:Cas9 ribonucleoprotein complex solution was incubated at 37°C for 5 min and then backfilled into 3 microinjection needles using an Eppendorf GELoader tip (Eppendorf, Cat# 022351656). After loading embryos into the embryo holder which covered with 12.5 ppt of salinity water with 0.0001% of methylene blue ...
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bioRxiv - Microbiology 2023Quote: ... CNW Technologies) using 80% ACN/5% NH4OH and dried at 45°C using a vacuum centrifuge (cat. no. 5305, Eppendorf, Hamburg, Germany). The peptide samples were resolved in 2% ACN/0.1FA for LC-MS analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were maintained between 10% and 80% confluence and kept at 37 °C with 5% CO2 (8% CO2 for VPC cells) in a humidified CO2 incubator (Eppendorf, Enfield, CT). The viable cell density (VCD ...
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bioRxiv - Microbiology 2024Quote: ... one with the blood mixed to RPMI and the second with the gametocyte suspension were spun down at 2000rpm for 5 minutes in a temperature-controlled centrifuge at 37°C (Eppendorf 5702 R). RPMI supernatant was removed from both tubes with 3ml disposable Pasteur pipettes ...
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bioRxiv - Biochemistry 2019Quote: Vero cells were harvested by trypsinization when it reached 80-90% confluence and centrifuged at 1000 × g for 5 mins (Eppendorf Centrifuge 5804R, Germany). The clean Vero cell pellet was resuspended thoroughly with growth medium (GM ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of the enrichment was transferred to 5 L NMS medium in a 6-L fermenter controlled with a BioFlo® 120 Bioprocess Control Station (Eppendorf, Hamburg, Germany). Gas stream carrying a relatively low CH4 concentration (0.5% v/v in air ...
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bioRxiv - Biophysics 2020Quote: ... Supplementary Table 5) were incubated together for 2 h at 37 °C under gentle shaking (450 rpm, Eppendorf ThermoMixer® C, Eppendorf AG, Germany). Unbound DNA origami was removed by placing the tube on a magnet and discarding the supernatant ...
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bioRxiv - Microbiology 2024Quote: ... and 40 mL of the supernatant was added as the inoculum to 5 L medium prepared in a BioFlo® 120 bioreactor (Eppendorf, Hamburg, Germany). Air containing 0.5% or 10% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were spun down (5 min, 800 g•, 2 °C, minimal acceleration and break, Eppendorf 5810 R with swing-×bucket rotor A-4-44). The medium was discarded and the cells were dissociated in 15 mL of ice cold ACK solution (0.15 M NH4Cl ...
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bioRxiv - Genetics 2021Quote: ... 72°C 45 s and a final extension at 72°C for 5 min was run on an Eppendorf Mastercycler® (Eppendorf AG, Hamburg Germany). Amplicons were observed on a 1.5% agarose gel (Bioline (Aust ...