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Citations for Eppendorf :
201 - 250 of 360 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: The homogenates were pelleted at 400xg for 6 minutes at 4°C in a swing-bucket rotor centrifuge (Eppendorf). The supernatants were removed and 1 ml ice-cold DPBS (pH 7.3-7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were put on ice for 30 min and precipitates were spun down for 5 min at 20,000 g at 4°C in a tabletop centrifuge (Eppendorf). After one wash with ice-cold acetone ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was subsequently centrifuged for 1 h at 11,000 x g at 4°C in an Eppendorf tabletop centrifuge 5810R (Eppendorf). The phage pellet was resuspended in 400 μl DNAseI buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then centrifuged at 13,000×g for 20 min at 4 °C using an F2402 rotor in a GS15R table-top centrifuge (Eppendorf). Pellets were air dried ...
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bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors and PMSF (10 minutes on ice) and centrifuged at 13,300 rpm for 5 min at 4OC (Centrifuge 5810 R; rotor A-4-81; Eppendorf). The supernatant (whole cell lysates ...
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bioRxiv - Cell Biology 2022Quote: ... 4 mM MgCl2) was carefully injected into an individual cardiomoycyte by a glass pipette via the CellTram injector (Eppendorf). After 5 minutes dye spreading to neighboring cardiomyocytes was visualized by Zeiss Axio Examiner A1 using a 40× objective and images were taken using TILL Photonics LA software (version 2.3.0.18 ...
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bioRxiv - Plant Biology 2023Quote: ... samples (stored for 4-12 weeks) were thawed at room temperature for 15 mins and centrifuged (Eppendorf Centrifuge 5417C) at 11,000 rpm for 14 mins to extract bulk leaf sap ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were centrifuged at 4°C at 15,000 RPM for 15 minutes in a table top centrifuge (Eppendorf 5424R). The supernatant’s protein concentration was measured using Bradford’s assay (Bio-Rad #5000202) ...
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bioRxiv - Bioengineering 2023Quote: All centrifugation experiments were conducted in an Eppendorf 5702R centrifuge with a swing bucket rotor (Eppendorf, A-4-38) at 20°C ...
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bioRxiv - Microbiology 2023Quote: ... harvested by centrifugation (3,500 × g for 20 min at 4°C, Centrifuge 5702 R, Eppendorf North America, Framingham, MA), and suspended in an equivalent amount of 1 × PBS ...
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bioRxiv - Microbiology 2023Quote: ... OD660 of 0.3-0.5 mid-log) were harvested by centrifugation (5,000 x g, 5 min at 4°C) (Eppendorf centrifuge 5430R). The supernatant was immediately discarded ...
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bioRxiv - Microbiology 2023Quote: ... The precipitated gDNA was pelleted by centrifugation (21,000 x g, 10 min at 4°C) in a tabletop centrifuge (Model 5424, Eppendorf). The supernatant was discarded by decantation ...
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bioRxiv - Neuroscience 2023Quote: ... and tubes centrifuged at 4 °C for 2 min at 12000 x g in a refrigerated microfuge (Eppendorf 5415R). The upper phase was discarded ...
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bioRxiv - Microbiology 2024Quote: ... the homogenate was centrifuged at 2500 rpm for 10 minutes at 4°C using a benchtop centrifuge (Eppendorf, USA), and the supernatant was collected.
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bioRxiv - Microbiology 2023Quote: ... The parasite solution was pelleted at 1,200 g at 4°C for 10 min using a chilled large tabletop centrifuge (Eppendorf). The resulting pellet underwent 3 washes with intracellular buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed in 20 mL of chilled phosphate-buffered saline (1X PBS, pH 7.4), and centrifuged (1500 ×g, 4 °C, 15 min) using the Centrifuge 5810R (Eppendorf). The cell pellet was subsequently resuspended in 30 mL of ice-cold RSB hypo buffer (10 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were centrifuged at 10,000 rpm at 4°C for 10 min and supernatants were dried under vacuum centrifugation (Eppendorf) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The incubated sample was centrifuged at 20,800 g for 15 min at 4 °C (Rotor F45-30-11, Eppendorf), and the resulting supernatant was analyzed by immunoblotting using various antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... fractions numbered from 9 to 22 were processed by centrifugation (20,800 g for 30 min at 4 °C, Rotor F45-30-11, Eppendorf), and the resulting pellet was further resuspended in 160 μL of alkaline carbonate buffer (100 mM Na2CO3 ...
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bioRxiv - Cell Biology 2020Quote: ... we centrifuged the crude lysate at 500 g for 5 min at 4°C in a microfuge (Eppendorf, model 5424R) to remove cell debris ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM label was subsequently added and samples were incubated over night at 4°C and 350 rpm in a Thermomixer (Eppendorf). Excess label was removed by using a Sephadex G25 column (GE Healthcare Bio-Science AB ...
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bioRxiv - Bioengineering 2022Quote: ... The lysates were clarified by centrifugation at 18,500g at 4 °C for 30 minutes using tabletop centrifuge (Eppendorf Centrifuge 5810R). Soluble protein was separated from cell debris and the supernatant was passed through 5 mL HisTrap columns (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... Single EGFP+ cells were sorted on a BD Influx equipped with a 140 µm nozzle and a cooling unit with a sample temperature of 4°C and collected into a DNA LoBind tube (Eppendorf) containing aCSF with 0.5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Absorbance was measured at 600 nm after every 12 h intervals till 4 to 5 days using a UV-visible spectrophotometer (Eppendorf).
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bioRxiv - Biophysics 2020Quote: ... The needle was then glued to a 6×4×2 mm Neodyn magnet (QM-06×04×02-N, Magnets4you) and attached to a motorized micromanipulator (PatchMan, Eppendorf). The magnetic needle was lowered until it touched the bottom of a dummy sample dish and raised again so that it was placed above the bottom of the sample with the very tip in focus around 100 μm above the focus of the glass surface.
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bioRxiv - Microbiology 2019Quote: ... the vials were removed from the incubator and beads were spun down at 1200 rpm using a swinging-bucket A-4-81 rotor (Eppendorf). An aliquot of 10 µl of bead suspension was carefully sampled from the bottom of the vials ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Genetics 2020Quote: ... The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042, Eppendorf, Inc.) and the resultant supernatant fractions were stored at −80°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were rotated at 4 °C for 5 minutes before being pelleted again with a swinging bucket centrifuge (500 x g, 5 min, 4°C; 5920R, Eppendorf). After centrifugation ...
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bioRxiv - Immunology 2021Quote: Xela DS2 (passages 120 – 130) and Xela VS2 (passages 125 – 135) (n = 4 independent trials) were seeded in a 6-well plate (Eppendorf) at a cell density of 625,000 cells/well in 1 mL of Xela complete media and allowed to adhere overnight at 26 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 180 μL aliquots of solubilized GlyT1 cell membrane were dispensed in a 4°C-cooled 96-well PCR plate (Eppendorf) in triplicates ...
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bioRxiv - Microbiology 2021Quote: ... Cell harvest was performed outside the anaerobic chamber at 3700 rpm for 12 min (Centrifuge 5920R, S-4×1000, Eppendorf) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transferred into the anaerobic chamber and harvested for 12 min at 25°C and 3700 rpm (Centrifuge 5920 R, S-4×1000, Eppendorf) outside of the anaerobic chamber ...
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bioRxiv - Neuroscience 2019Quote: ... The lysate was cleared from the insoluble fraction by centrifugation for 15 min at 10,000 rpm (11,292 x g) at 4 °C (Eppendorf 5427 R). The supernatant was retrieved and the procedure repeated until no pellet was visible after centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... The reactions were stopped by the addition of phosphoric acid and 4 µl of each reaction were spotted on P81 phosphocellulose papers (Whatman) using the epMotion 5070 (Eppendorf) workstation ...
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bioRxiv - Cell Biology 2021Quote: ... was harvested by centrifugation at 300 × g for 5 min in a swing out 5804 S-4-72 rotor (Eppendorf). Baculovirus infected insect cell (BIIC ...
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bioRxiv - Microbiology 2019Quote: ... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... were vortexed 3 min at room temperature followed by centrifugation at 4,500 x g for 5 min at 4°C (Eppendorf #5010R). The supernatant fluid was poured into a chilled 2 ml screw-capped tube ...
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bioRxiv - Cell Biology 2021Quote: ... The resuspended cilia sample was centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Synthetic Biology 2022Quote: The concentration and quality of the plasmid solutions were determined by diluting 1 ul of plasmid solution in 4 ul of Tris EDTA buffer and loading 3 ul of the diluted solution on a μCuvette G1 (Eppendorf). Optical densities were measured at 260 nm and 280 nm using a BioSpectrophotometer and Fluorimeter (Eppendorf) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasma was defrosted and then centrifuged at 2000 rpm (805 × g) for 4 min in a 5810 R centrifuge (Eppendorf) to remove possible particulate matter ...
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bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Homogenates were centrifuged at 1000 rpm for 5 min at 4°C in a table centrifuge (5424 R, Eppendorf, Germany). The supernatant was transferred to a new tube and again centrifuged at 4°C for 5 min at 1400 rpm ...
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bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Bioengineering 2019Quote: ... the crude extracts were transferred into a fresh tube and centrifuged for 30 min at 15000g and 4 °C (Centrifuge 5427R, Eppendorf). The soluble supernatant (blue color ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Peptides were eluted in 20 μL of 80% acetonitrile in 0.1% TFA and were concentrated to 4 μL (Concentrator 5301, Eppendorf AG). The peptides sample was then diluted to 5 μL by 0.1% TFA for LC-MS/MS analysis.
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bioRxiv - Cell Biology 2022Quote: ... Sorted nuclei were then centrifuged at 1000 g for 15 min (Eppendorf, 5920R; 4°C, ramp speed of 3/3), and supernatant was removed ...