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Citations for Eppendorf :
151 - 200 of 297 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and re-dissolved in lysis buffer supplemented with 1% v/v SDS at RT in a Thermomixer R (Eppendorf) at 1200 rpm for 1-2 hours ...
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bioRxiv - Biophysics 2020Quote: ... or 1 mM LY294,002 and 10 μg/ml Alexa594 were prepared in DB and loaded into Femtotips II (Eppendorf). The tip mounted on the micromanipulator (TransferMan 4r or TransferMan NK2 ...
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bioRxiv - Biochemistry 2022Quote: ... The rest of the solution was incubated at 4°C for 1 hour on a thermomixer (Eppendorf, Hamburg, Germany) set to 1000 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Organ samples were weighed and triturated in 1 ml sterile tissue culture media using a tissue homogenizer (Eppendorf, Germany). Two hundred microliters of the tissue homogenate/ swab specimens were used for RNA extraction using MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: 30 asteroids per condition were pooled in a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Microbiology 2021Quote: ... cells equivalent to 1 OD600 were pelleted by centrifugation at 2,808 x g for 10 min (Eppendorf 5810 R) and the pellets were flash frozen ...
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bioRxiv - Genomics 2019Quote: ... Beads were then resuspended in 200 uL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65°C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies from chromatin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The sample was subsequently centrifuged for 1 h at 11,000 x g at 4°C in an Eppendorf tabletop centrifuge 5810R (Eppendorf). The phage pellet was resuspended in 400 μl DNAseI buffer (10 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2022Quote: Oocyte and 1-cell embryo microinjections were performed using a Femtojet injector and TransferMan 4r micromanipulators (Eppendorf, Enfield, CT) on a Leica DMI 6000B inverted microscope ...
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bioRxiv - Genetics 2022Quote: ... StageTips were washed twice with 0.1% FA and peptides were eluted with 40% ACN/0.1% FA and then dried in a Speed-Vac (Eppendorf) at 45°C for 45 min.
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bioRxiv - Molecular Biology 2022Quote: ... aprotinin1µg/mL and pepstatin 1 µg/mL) and twice with RIPA containing NaCl 350 mM in LowBind tubes (Eppendorf). Elution of SUMOylated proteins was performed twice with peptides bearing either the 21C7 SUMO-1- (VPMNSLRFLFE ...
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bioRxiv - Genomics 2022Quote: ... and the cell pellet was washed with 1 ml of DPBS and 0.01% BSA and then transferred to a 1.5 ml DNA LoBind tube (Eppendorf, #022431021). Cells were pelleted again and resuspended in DPBS and 0.01% BSA ...
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bioRxiv - Systems Biology 2023Quote: ... washed five times with 1 mL 0.1% TFA in water and peptides were eluted with 70% ACN/0.1% TFA (1 mL) and dried in vacuo in a Concentrator plus (Eppendorf).
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bioRxiv - Cell Biology 2023Quote: ... Triton ×100 was added (final concentration 1%) to the sonicated chromatin and moved to a low-retention tube (Eppendorf) before centrifugation at 14,000 rpm for 15min at 4C and pellets were discarded ...
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bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 200 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and incubated at 65 °C in a Thermomixer (Eppendorf) with interval mixing to dissociate antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was sonicated 3 times for 1 minute in a waterbath sonicator and incubated in a ThermoMixer (Eppendorf) for 30 minutes at 37°C and 500 rpm ...
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bioRxiv - Microbiology 2023Quote: ... or in a hypoxic environment in the presence of 1% O2 in humidified CO2 chamber (New Brunswick Scientific/Eppendorf) or in the presence of 300 mM CoCl2 (Sigma-Aldrich/Merck) ...
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bioRxiv - Genomics 2024Quote: ... the 1*105 cells were taken off for ATAC sequencing and placed into DNA LoBind 1.5mL tubes (Eppendorf, 2231000945). The cells were washed 3x in ice cold buffered natrium chloride (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were run in simplex at 1 and 10-fold dilutions on a Mastercycler® Nexus Gradient (Eppendorf, Germany). Optimised PCR Ta were compared to Ta=50°C as used by Ollivier et al ...
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bioRxiv - Molecular Biology 2023Quote: ... 66 Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf). They were then prepared for LC-MS/MS analysis by diluting it to 5 μl by 0.1% TFA.
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bioRxiv - Cell Biology 2020Quote: ... Peptides were eluted in 40 μl of 80% acetonitrile in 0.1% TFA and concentrated down to 1 μl by vacuum centrifugation (Concentrator 5301, Eppendorf, UK). Samples were then prepared for LC-MS/MS analysis by diluting them to 5 μl with 0.1% TFA ...
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bioRxiv - Cell Biology 2019Quote: ... 1 nl of morpholino or mRNA diluted in nuclease-free water at desired concentrations were microinjected into the yolk along with 1% phenol red dye using FemtoJet microinjector (Eppendorf). Microinjections for rescue experiments were performed as two separate sets with initial mRNA injections followed by morpholino injections in the same embryo ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa SS6 or 1.E cells were seeded into 35 mm tissue culture dishes or 6 well tissue culture plates (Eppendorf) with or without pre-cleaned ...
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bioRxiv - Developmental Biology 2021Quote: ... Japan) followed by a 1-hr incubation at 25 °C with intermittent vortexing using a thermomixer (Eppendorf, Wesseling-Berzdorf, Germany). Aliquots were designated as the RIPA-insoluble fraction and subsequently mixed with LDS sample buffer and boiled at 95 °C for 3 min before being stocked frozen at -30 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... diluted 1:250 in 500ul PBSFBT either ON or for 2h at 32°C in a heating block (ThermoMixer C, Eppendorf) with integrated shaking (350rpm) ...
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bioRxiv - Developmental Biology 2021Quote: ... with a titer of 1×109 TU/ml were injected into the sub-germinal cavity using an oil hydraulic injection system (CellTram oil, Eppendorf). Before the injection ...
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bioRxiv - Biochemistry 2022Quote: ... at a final concentration of 1 mM for 30 min at 30 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). The reaction was quenched with ammonium bicarbonate at a final concentration of 50 mM for 10 min at 30 °C and 650 rpm ...
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bioRxiv - Immunology 2019Quote: ... six cycles 94°C for 20 sec and a touchdown program of 15 cycles with successive annealing temperature decrement from 65°C to 60°C for 30 sec with 1°C decrement with a final extension at 72°C 10 min performed in a Mastercycler Gradient (Eppendorf). The products were monitored using 1.5 % agarose gel electrophoresis stained with ethidium bromide ...
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bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... cerevisiae CEN.PK-113-7D were performed in a 1 l working volume of a stirred fermenter (DASGIP parallel bioreactor system, Eppendorf). Throughout the fermentations the pH was kept at 5.1 and the temperature was kept at 30 degrees Celsius ...
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bioRxiv - Neuroscience 2020Quote: ... the pellet was resuspended in SK buffer (LS buffer, 1% sarkosyl, 500mM NaCl) and solubilized at 22°C for 60 minutes using a ThermoMixer 5350 (Eppendorf) at 500rpm ...
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bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
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bioRxiv - Plant Biology 2020Quote: ... Each insoluble fraction was digested by 0.5 μg chymotrypsin and 1 μg trypsin at 37°C for 20 h shaking at 600 rpm (Eppendorf tabletop). Digestion reactions were stopped using TFA to a final concentration of 0.5% ...
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bioRxiv - Synthetic Biology 2021Quote: ... The fractions with highest concentrations (200–400 μg mL−1) were stored as 5–10 μL aliquots in Protein LoBind tubes (Eppendorf) at –80 °C.
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bioRxiv - Cancer Biology 2020Quote: ... at a concentration of 100μg/ml for 1 hr at 37°C in a heat block (Thermo mixer, Eppendorf, Germany). After incubation 10μg/ml of propidium iodide was added in dark for 15 min ...
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bioRxiv - Biophysics 2022Quote: ... the DNA droplet-containing aqueous phase was layered on top of the oil phase in a volumetric ratio of 1:3 within a microtube (Eppendorf). For the segregation of DNA droplets using enzymatic activity ...
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bioRxiv - Neuroscience 2022Quote: ... Lysate was spun at 800 g for 10 min (spin 1) to remove cell debris (Eppendorf Centrifuges 5430 with Eppendorf FA-45-48-11 30-spot 45-degree fixed angel rotor) ...
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bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
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bioRxiv - Biophysics 2022Quote: ... The aqueous solution was layered on top of the oil-surfactant mix in a volumetric ratio of 1:3 inside a microtube (Eppendorf). The tube was manually shaken for about 30 s until water-in-oil droplets formed ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were cleaned up with 2x 100 µl 99% ddH2O 1% TFA into 2x 100 µl 99% Isopropanol 1% TFA at 1200 xg at RT each using a table-top centrifuge (Eppendorf). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... The fully dried gel slices were incubated with 1 µg trypsin per tube in 50 mM ammonium bicarbonate buffer at 37°C overnight on a ThermoMixer (Eppendorf). Digested peptides were extracted twice with 150 µl of 30% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... frozen leaves were extracted in 50 mM P buffer (pH 7.5) at a ratio of 1:8 (w/v) and centrifuged (Eppendorf 5417R) twice at 18,000g for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were digested with trypsin (1:50 trypsin:protein ratio) overnight at 37°C with light agitation (450 rpm on a Eppendorf thermomixer). Digestion was stopped by the addition of trifluoracetic acid (TFA ...
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bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
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bioRxiv - Biochemistry 2019Quote: ... Varying volumes of viral supernatant were mixed with 5 × 106 to 1 × 107 log phase Jurkat cells in a final volume of 10 ml within a T-25 flask (Eppendorf) and subsequently cultured for 72 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... albeit the injected droplets were collected in fractions of ∼1000 cells (∼27 µl of loaded droplets) in 1 ml LoBind tubes (Eppendorf) pre-filled with 200 µl of mineral oil ...
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bioRxiv - Developmental Biology 2020Quote: ... Pairs of PPFs were isolated manually with forceps from the lateral body wall and from the underlying septum transversum and liver (Video 1) and then each pair placed in a single well in a 96-well plate (Eppendorf) containing 100 μL of media ...
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bioRxiv - Biochemistry 2020Quote: ... chloroform (4:2:1, v: v: v, containing 20 mM ammonium formate. The solutions were then placed into the wells of an Eppendorf twin-tec 96-well PCR plate ...
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bioRxiv - Genomics 2019Quote: ... Deep red-) Single cells were sorted into 5 ul of RLT 1%β-Mercaptoethanol in a 96 well plate (Eppendorf) and frozen at −80°C.
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bioRxiv - Biochemistry 2019Quote: ... and plasma and bone marrow interstitial fluid samples from three pediatric B-ALL patients (B-ALL-1, −2, −3) were processed on an epMotion M5073 automated liquid handling system (Eppendorf) controlled by an EasyCon tablet (Eppendorf) ...