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Citations for Eppendorf :
501 - 550 of 672 citations for 8 4 Chlorophenylthio guanosine 3' 5' cyclic Monophosphate triethylammonium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
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bioRxiv - Systems Biology 2020Quote: ... A fixed volume of 100 μL cells was used to inoculate 50 mL preculture M9 +40 mM MOPS media with 4 g/L glucose which was grown overnight in a shake flask at 200 rpm in a 37°C (Eppendorf) incubator ...
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bioRxiv - Neuroscience 2019Quote: ... Excised gel slices were incubated with 50% acetonitrile/50mM ammonium bicarbonate for 2 h at room temperature or 4°C overnight with gentle shaking at 600 rpm in a Thermomixer (Eppendorf), before being incubated in 100% acetonitrile for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Bioengineering 2019Quote: ... the crude extracts were transferred into a fresh tube and centrifuged for 30 min at 15000g and 4 °C (Centrifuge 5427R, Eppendorf). The soluble supernatant (blue color ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were then centrifuged at 400xg for 10 min (Eppendorf Centrifuge 5810R, rotor A-4-62) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Peptides were eluted in 20 μL of 80% acetonitrile in 0.1% TFA and were concentrated to 4 μL (Concentrator 5301, Eppendorf AG). The peptides sample was then diluted to 5 μL by 0.1% TFA for LC-MS/MS analysis.
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged at 16,100 g for 20 min at 4 °C with an Eppendorf 5415R centrifuge (Eppendorf, Hamburg, Germany), sonicated for 10 s and protein amounts normalized following a bicinchoninic acid protein assay (ThermoFisher) ...
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bioRxiv - Genomics 2022Quote: ... The beads were then incubated 1 h with the anti-Myc antibody (9E10) at 4°C (Eppendorf ThermoMixer, 1,300 rpm), washed three times in PBS/0.1 % BSA and two times in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
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bioRxiv - Microbiology 2023Quote: ... The sample was vortexed for 30 seconds before being centrifuged for 15 mins at 4°C at 1739 x g (3000 rpm, Eppendorf 5810R benchtop centrifuge ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was then incubated at 37 °C for 4 hrs under gentle orbital agitation (300 rpm on Eppendorf Thermomixer) and centrifuged at 15 000 × g for 5 min prior to SEC analysis ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the samples were 10000xg centrifuged for 10 min in a cooled centrifuge (4°C; Centrifuge 5415 R, Eppendorf, Hamburg, Germany), and the supernatant transferred to new microcentrifuge tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... After centrifugation for 10 min at 15,000 x g and 4 °C with a 5415R benchtop microcentrifuge (Eppendorf, Hamburg, Germany), the supernatants were collected and residual sample pellets were reextracted with a 200 μL aliquot of the previously used extraction solvent mixture ...
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bioRxiv - Developmental Biology 2023Quote: ... About 6 pl dCas9-KRAB mRNA and sgRNA mixes were injected into cytoplasm of zygotes between 2-4 hpi using a Piezo impact-driven micromanipulator (Eppendorf). For all micro-injection experiments ...
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bioRxiv - Cell Biology 2023Quote: ... followed by ϕ29 polymerase heat inactivation at 65°C for 20 min and cooling at 4°C using PCR thermocycler (Eppendorf). Amplified C-circle DNA was diluted with 2X SSC buffer and loaded onto a dot-blot apparatus bedded with Hybond N+ membrane (RPN2020B ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells were sorted using a BD FACS Aria cell sorter at 4°C and collected in 0.4 mg/mL BSA in DNA LowBind tubes (Eppendorf, 0030108523). Cells were pelleted at 300 g for 5 minutes at 4°C and manually counted using a Bürker Chamber ...
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bioRxiv - Evolutionary Biology 2024Quote: 15mL of a Salten solution (1011 PFU/mL) was distributed into 1.5mL polypropylene tubes and centrifuged for 1 hour at 16,000g at 4°C (Eppendorf Centrifuge 5415R). Pellets were resuspended in 100µL of 100 mM of ammonium acetate (NH4-CH3CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM D-L methionine) for 1 h at 4° C with shaking at 1400 rpm in a benchtop thermomixer 22331(Eppendorf). The beads were washed twice with binding buffer without soybean trypsin inhibitor and suspended in 50 % v/v binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min-4 h at 76°C in a heat block (speed of 300 rpm; ThermoMixer F1.5, Eppendorf, Germany) (Wagner et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were then pelleted by centrifugation at 3900 × g for 20 min (5810R centrifuge with S-4-104 rotor, Eppendorf) and stored at −20 °C.
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bioRxiv - Synthetic Biology 2024Quote: ... + 90% air or 10% 12CO2 + 90% air generated by a DASGIP MX4/4 stand-alone gas-mixing module (Eppendorf, Germany). The flasks were placed in a 37°C shaker incubator ...
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bioRxiv - Bioengineering 2020Quote: ... were centrifuged at 16,873 gav for 3 min in the FA-45-18-11 fixed angle (45°) rotor of an Eppendorf model 5418 centrifuge (Eppendorf AG, Hamburg, Germany). The pellets were resuspended in 1 mL of 2.5% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... MPCs were washed twice for 3 min at 400 RCF at room temperature with PBS supplemented with 1% glucose and 1% pen-strep (Eppendorf 5702R, Hamburg, Germany). Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher ...
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bioRxiv - Genetics 2019Quote: ... Dry extracts were dissolved in 2 ml methanol and transferred to 4 ml glass vials prior of being concentrated to dryness in a speed vacuum (Speed Vac-Concentrator, Eppendorf, Hamburg) for 2 h at 28 °C ...
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A DNA-based optical nanosensor for in vivo imaging of acetylcholine in the peripheral nervous systembioRxiv - Bioengineering 2020Quote: The DNA scaffold was self-assembled by incubating 5 DNA oligonucleotides following a temperature gradient from 95 °C to 4 °C for 6 h in a thermocycler (Mastercycler® nexus X2, Eppendorf) in Tris-EDTA buffer (TE buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... To reduce non-specific binding of aggregates to the DNA beads, the HSS was centrifuged (14000 rpm, 10 min., 4°C) in a fixed angle microfuge (Eppendorf 5418). The HSS was then split into two 50 µL samples and the DNA-PKcs inhibitor NU-7441 (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... an aliquot of the culture was removed for CFU determination by plate counting and the remainder was centrifuged at 4°C and 3,100 rpm (Eppendorf Centrifuge 5415R) for 10 min to generate cell-free supernatants ...
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bioRxiv - Neuroscience 2020Quote: ... 200 mM solution in water) was then added to the samples and incubated for 4 hours at 21°C in a ThermoMixer (Eppendorf AG). 4 µL of 1M IAA (iodoacetamide in water ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were incubated on ice for 30 min before the demembraned cilia were centrifuged at 16,000g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415D). The supernatant was discarded ...
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bioRxiv - Microbiology 2021Quote: ... Serum was separated from blood by centrifugation at 8,000 RPM for 30 minutes at 4 °C in an Eppendorf 5424R centrifuge (Eppendorf, Enfield, CT). Serum was heat inactivated by incubating at 56 °C for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with immunized vaccine antigen and incubated for two hours at 25 °C (4 μg/mL, in 1xPBS, 50 μL/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). ACE2-hFc protein coating was used as a control for antigen immobilization ...
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bioRxiv - Biophysics 2020Quote: 96 well plates were coated with vaccine antigen and incubated overnight at 25 °C (4 μg/mL in 1x PBS, 50 μl/well) under constant shaking (300 rpm) on a MixMate thermomixer (Eppendorf, USA). Ovalbumin (4 μg/mL in 1x PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... Solutions were then spun down at 13,000 RPM for 30 minutes at 4°C in a benchtop micro-centrifuge (Eppendorf Centrifuge 5424) to remove cells and other solid debris ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were centrifuged at 14k rpm for 15 minutes at 4°C and the aqueous phase was collected into clean 1.5 mL Eppendorf tubes (Eppendorf, Hauppauge, NY). 500 µL of ice-cold isopropanol was added to each tube ...
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bioRxiv - Microbiology 2020Quote: ... The cultures were grown in BHI broth for 24 h and were centrifuged at 3200 × g for 20 min at 4°C (Eppendorf, 5424R). The cell pellets were transported on ice for proteome analysis at The Mass Spectrometry Centre ...
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bioRxiv - Bioengineering 2020Quote: ... Cotton swabs containing simulated forensic samples were placed in 500 μL of 1X PBS and mixed at 4°C in a Thermomixer (Eppendorf, Germany) set at 1,000 rpm for approximately an hour ...
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bioRxiv - Bioengineering 2020Quote: ... and microbubbles were isolated by 4 centrifugation wash cycles at 40 relative centrifugation force for 1 min (Eppendorf 5804, Hamburg, Germany), where the infranatant was discarded and the microbubble concentrate was saved and resuspended.
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bioRxiv - Neuroscience 2022Quote: ... 3 times with 10” ON and 30” OFF intervals on 20% power on ice and cleared by centrifugation at 21,000 rpm for 20min at 4°C (Eppendorf 5424R Centrifuge). 50µg of supernatant was mixed with NuPAGE LDS Sample Buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Blood samples were collected on ice and centrifuged within 1h at 4°C for 10min at 3000 RPM (Eppendorf, Centrifuge 5804R). Separated plasma was stored at -80°C until the protein assay was initiated.
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bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
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bioRxiv - Developmental Biology 2023Quote: The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Control embryos were injected with Cas9 protein and Lap2b-GFP mRNA only.
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bioRxiv - Biophysics 2023Quote: ... Lysate and cell fragments were separated by centrifugation at 4 °C for 20 min at 12,000 rpm (Centrifuge 5418 R, Eppendorf, Hamburg, Germany). Protein concentrations in the supernatant were determined using the Pierce Micro BCA Protein Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The cell culture supernatant was collected and pre-cleared from cells and larger particles by centrifugation at 400 × g at 4 °C for 10 min (Eppendorf 5810R) and 10,000 × g at 4 °C for 30 min (Eppendorf 5910R) ...
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bioRxiv - Biochemistry 2023Quote: ... in 5% glucose solution v/v prior to adding 2X solubilization buffer and incubation at 37 °C for 4 hrs under gentle orbital agitation (500 rpm on Eppendorf Thermomixer). The product was then analyzed by SEC as described earlier.
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bioRxiv - Developmental Biology 2022Quote: ... The filled needle is positioned on a micromanipulator (Narishige MMO-4) and connected to a positive pressure pump (Eppendorf FemtoJet 4i). Embryos are placed in FHM drops covered with mineral oil under Leica TL Led microscope ...
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bioRxiv - Biophysics 2023Quote: ... 4 µl ATP from the dilution series prepared for the master mix plate (Eppendorf tubes with maximum ATP conc. = 200 µM) were added to row A and row I on reaction stop plate ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for 1 hour at a temperature of 4°C with shaking at 600 rpm in a Thermomixer comfort (Eppendorf, Germany). Stained cells were washed with ice cold FACS buffer twice before resuspension in FACS buffer for analysis ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was used to fractionate the cell lysate (at 4 °C for 30 min at 4,416 g, Eppendorf, Centrifuge 5804 R) and at 4 °C for 1 hour for ultracentrifugation (70,658 g ...